Figure 3. Direct Hairy target genes exhibit broad domains of chromatin effects.
Distribution of genome-averaged ChIP-seq signals before (straight line) and after (dashed line) Hairy induction, showing 4 kb window around affected regions. (A) Distributions of histone H4Ac and H3K27Ac marks of direct Hairy targets were significantly broader than for regions (B) not bound by Hairy (p = 2.55e-92 and p = 5.63e-70 respectively; KM test).
Figure 3—figure supplement 1. Distinct chromatin profiles associated with direct and indirect Hairy targeted loci.
Histograms show the distribution of averaged ChIP-seq signals in a window of 4 kb centered on differentially changed regions associated with Hairy bound (A) and unbound (B) genes in the wild-type (wt, solid lines) and Hairy induced (H, dashed lines) embryos for H4Ac, H3K27Ac, H3K4me1, H3K4me3, H3K36me3 and H3K9me3.
Figure 3—figure supplement 2. Little correlation between height of Hairy peaks or width of Hairy-bound region and extent of H4 deacetylation blocks and width (A) or height (B) of Hairy peaks.
Other marks also exhibited little correlation between Hairy peak width and height and range of chromatin alterations (not shown).