There is an error in Fig 3. Fig. 2A has been incorrectly repeated. Please view the complete, correct Fig 3 here.
Fig 3. Stimulation of TNF-α production in RAW 264.7 mouse macrophages by lipoprotein-enriched cell membrane fractions of E. faecalis wild type and ΔbgsA.

RAW 264.7 cells were incubated with lipoprotein-enriched Triton X-114 extracts from total membrane protein fractions derived from the indicated E. faecalis strains. The concentration of lipoprotein extracts was measured photometrically and normalized to a bacterial cfu:RAW 264.7 cell ratio of 10,000:1. At 16 h, supernatants were collected and TNF-α concentrations were quantified by ELISA. LPS at a concentration of 100 ng/ml was used as positive control. Data represent mean ± SEM of triplicates. * p < 0.001 12030 ΔbgsA versus 12030 WT.
Reference
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