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. 1988 Nov;7(11):3309–3314. doi: 10.1002/j.1460-2075.1988.tb03201.x

A new locus (NIA 1) in Arabidopsis thaliana encoding nitrate reductase.

C L Cheng 1, J Dewdney 1, H G Nam 1, B G den Boer 1, H M Goodman 1
PMCID: PMC454825  PMID: 2905260

Abstract

We have isolated two nitrate reductase genes and their corresponding cDNAs from Arabidopsis thaliana. Sequences of the two cDNAs, when compared to a sequence of a barley cDNA clone, confirm their identity as nitrate reductase clones and show that they are closely related. The two genes have been mapped using restriction fragment length polymorphisms; gNR2 is close to the previously identified chl-3 locus and is probably identical to it, while gNR1 maps to a new locus (NIA1) on chromosome 1, near gl-2.

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Selected References

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  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calza R, Huttner E, Vincentz M, Rouzé P, Galangau F, Vaucheret H, Chérel I, Meyer C, Kronenberger J, Caboche M. Cloning of DNA fragments complementary to tobacco nitrate reductase mRNA and encoding epitopes common to the nitrate reductases from higher plants. Mol Gen Genet. 1987 Oct;209(3):552–562. doi: 10.1007/BF00331162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cashmore A. R. Reiteration frequency of the gene coding for the small subunit of ribulose--1,5--bisphosphate carboxylase. Cell. 1979 Jun;17(2):383–388. doi: 10.1016/0092-8674(79)90164-8. [DOI] [PubMed] [Google Scholar]
  4. Cheng C. L., Dewdney J., Kleinhofs A., Goodman H. M. Cloning and nitrate induction of nitrate reductase mRNA. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6825–6828. doi: 10.1073/pnas.83.18.6825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crawford N. M., Campbell W. H., Davis R. W. Nitrate reductase from squash: cDNA cloning and nitrate regulation. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8073–8076. doi: 10.1073/pnas.83.21.8073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dailey F. A., Kuo T., Warner R. L. Pyridine nucleotide specificity of barley nitrate reductase. Plant Physiol. 1982 May;69(5):1196–1199. doi: 10.1104/pp.69.5.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  9. Feramisco J. R., Smart J. E., Burridge K., Helfman D. M., Thomas G. P. Co-existence of vinculin and a vinculin-like protein of higher molecular weight in smooth muscle. J Biol Chem. 1982 Sep 25;257(18):11024–11031. [PubMed] [Google Scholar]
  10. Gabard J, Marion-Poll A, Chérel I, Meyer C, Müller A, Caboche M. Isolation and characterization of Nicotiana plumbaginifolia nitrate reductase-deficient mutants: genetic and biochemical analysis of the NIA complementation group. Mol Gen Genet. 1987 Oct;209(3):596–606. doi: 10.1007/BF00331169. [DOI] [PubMed] [Google Scholar]
  11. Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
  12. Hagberg L., Hull R., Hull S., Falkow S., Freter R., Svanborg Edén C. Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983 Apr;40(1):265–272. doi: 10.1128/iai.40.1.265-272.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jolly S. O., Campbell W., Tolbert N. E. NADPH- and NADH-nitrate reductases from soybean leaves. Arch Biochem Biophys. 1976 Jun;174(2):431–439. doi: 10.1016/0003-9861(76)90371-4. [DOI] [PubMed] [Google Scholar]
  14. Maas R. An improved colony hybridization method with significantly increased sensitivity for detection of single genes. Plasmid. 1983 Nov;10(3):296–298. doi: 10.1016/0147-619x(83)90045-8. [DOI] [PubMed] [Google Scholar]
  15. Nakagawa H., Yonemura Y., Yamamoto H., Sato T., Ogura N., Sato R. Spinach nitrate reductase: purification, molecular weight, and subunit composition. Plant Physiol. 1985 Jan;77(1):124–128. doi: 10.1104/pp.77.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ross J., Aviv H., Scolnick E., Leder P. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer). Proc Natl Acad Sci U S A. 1972 Jan;69(1):264–268. doi: 10.1073/pnas.69.1.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shen T. C., Funkhouser E. A., Guerrero M. G. NADH- and NAD(P)H-Nitrate Reductases in Rice Seedlings. Plant Physiol. 1976 Sep;58(3):292–294. doi: 10.1104/pp.58.3.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Solomonson L. P. Purification of NADH-Nitrate Reductase by Affinity Chromatography. Plant Physiol. 1975 Dec;56(6):853–855. doi: 10.1104/pp.56.6.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Somers D. A., Kuo T. M., Kleinhofs A., Warner R. L., Oaks A. Synthesis and degradation of barley nitrate reductase. Plant Physiol. 1983 Aug;72(4):949–952. doi: 10.1104/pp.72.4.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Streit L., Martin B. A., Harper J. E. A method for the separation and partial purification of the three forms of nitrate reductase present in wild-type soybean leaves. Plant Physiol. 1987 Jul;84(3):654–657. doi: 10.1104/pp.84.3.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Streit L., Nelson R. S., Harper J. E. Nitrate Reductases from Wild-Type and nr(1)-Mutant Soybean (Glycine max [L.] Merr.) Leaves : I. Purification, Kinetics, and Physical Properties. Plant Physiol. 1985 May;78(1):80–84. doi: 10.1104/pp.78.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]

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