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. Author manuscript; available in PMC: 2015 Aug 25.
Published in final edited form as: Oncogene. 2009 Aug 17;28(44):3880–3891. doi: 10.1038/onc.2009.242

Figure 4.

Figure 4

Influence of MFN1/2,OPA1 and DRP1 on DXR induced nucleoid remodelling. (A left) Anti-DNA IgM1 labelling of DXR/vehicle treated wild-type, MFN1 null and MFN2 null MEFs (7.2 μM DXR 24 hrs). (A right) PicoGreen labelling of fibroblasts transfected with either OPA1 targeting siRNA (48 hours) or a scramble siRNA control, after treatment with DXR (7.2 μM 24 hours). (A graph) Quantification of % cells with DXR induced remodelled nucleoids (n = 200). (B) Representative brdU labelling (20μM 24 hours) of wild-type, MFN1 and MFN2 null cells, treated with vehicle/DXR (3.4 μM 24 hours). (C) PicoGreen/TMRM labelling of DRP1/Scramble siRNA treated cells (6 days). Bars 20 μm. Results + S.D. Results are representative of three independent experiments.