FIG 1 .

Biofilm formation by Pseudomonas aeruginosa PA14 exposed to C-type natriuretic peptide (CNP) or brain natriuretic peptide (BNP) in dynamic condition. (A) COMSTAT analyses of biofilms of P. aeruginosa PA14 control (PA14) or PA14 exposed to CNP (10⁻⁶ M) or BNP (10⁻⁶ M). Data are the means for twelve samples from six independent experiments for control bacteria, ten samples from six independent experiments for CNP-exposed bacteria, and six samples from two independent experiments for BNP-exposed bacteria. ***, P < 0.001. (B) 3D shadow representations of the biofilm structures developed under dynamic condition by P. aeruginosa PA14 control or PA14 exposed to CNP or exposed to BNP at 37°C for 24 h in LB broth. Biofilms were stained with Syto 61 red dye or SytoX dye and observed by confocal laser scanning microscopy.