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. 1994 Dec 20;91(26):12745–12749. doi: 10.1073/pnas.91.26.12745

Pax-3 contains domains for transcription activation and transcription inhibition.

G Chalepakis 1, F S Jones 1, G M Edelman 1, P Gruss 1
PMCID: PMC45516  PMID: 7809114

Abstract

Pax-3 is a member of the Pax family of transcription factors involved in transcriptional control events during embryonic development. Here we report a functional dissection of the Pax-3 protein and describe the protein domains which are responsible for different activities. A transcription inhibition activity is located in the first 90 N-terminal amino acids and includes part of the paired domain. Furthermore, the C terminus of Pax-3 is able to confer transcriptional activation of basal promoters. Pax-3 can utilize both transcription modulating functions and activates transcription over a narrow range of protein concentration in the presence of promoter elements containing functional binding sites.

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