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Phase-contrast micrographs of ΔccrM strains with single point mutations 7 generations after transfer from minimal to rich medium. Cells from strains JC1323, JC1325, JC1327, JC1328, JC1336, JC1338, and JC1343 are shown. Compare these images with the micrographs of a ΔccrM population after ~6 generations in rich medium (PYE) (Fig. 1B). The morphology of the same mutants in minimal medium (M2G) and at later generations in rich medium is presented in Fig. S2 in the supplemental material.
