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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Theriogenology. 2015 Jun 12;84(6):1024–1032. doi: 10.1016/j.theriogenology.2015.05.036

Table 2.

Incubation of TPEN can increase development potential of activated oocytes. Incubating activated porcine oocytes with lower concentrations of TPEN (5-10 μM) increased formation of blastocysts. Different letters indicate statistical difference (p<0.05). Frequency of blastocysts was recorded on Day 6.

Treatment Total number of embryos % cleaved % Blastocysts Total cell number in blastocysts
THI/DTT 113 59.3a 10.6a 28.2 ± 2.1a
THI/DTT + 5 μM TPEN, 30 min 114 74.6b 27.2b 33.1 ± 2.6b
THI/DTT + 10 μM TPEN, 10 min 83 81.9b 21.7b 31.2 ± 2.6b