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. 2012 May 7;3(2):473–491. doi: 10.3390/insects3020473

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Phylogenetic position of the bacteria analyzed in the present study (horizontal arrows), based on partial 16S rRNA sequences (dataset ‘bacterial phylogeny’). The tree was constructed using the RNA7D model in PHASE and rooted at the base of a clade consisting of the beta- and gamma-proteobacteria. It represents a 50% majority rule tree with branch lengths estimated by the software. GenBank accession numbers are given. ‘Symb._of’ means ‘symbiont of’. The ‘focal clade’ is the branch that contains the symbionts of the Donaciinae. It does not have a formal systematic meaning, and its members are not identical in our two datasets (see text).