There are errors in the seventh sentence of the Abstract. Specifically, the values provided for ATP, BBG and OATP are incorrect. The correct sentence is: The values obtained were consistent with those found in the literature (0.7 ± 0.07 mM, 1.3–2.6 μM and 173–285 μM for ATP, BBG and OATP, respectively).
Additionally, there are errors in the units of Table 1. The correct units for columns BBG (IC50) and OATP (IC50) are μM. Please see the corrected Table 1 here.
Table 1. IC50 or EC50 values for P2X7R agonists or antagonists using different methods.
| ATP (EC50) | BBG (IC50)* | OATP (IC50) | Reference | |
|---|---|---|---|---|
| Cytometry | 2–4 mM | N.D.** | 300 μM | Coddou et al. 2011 |
| Electrophysiology | 2–4 mM | N.D.*** | 300 μM | Coddou et al. 2011 |
| FLIPR | N.D.** | 6.71±0.05 μM | N.D.** | Namovic et al. 2012 |
| Spectrophotometry | 0.7 ±0.07 mM | 1.3–2.6 μM | 173–285 μM | Present study |
*IC50 for natively expressed mP2X7R in J774.G8 cells.
**N.D. = Not determined
Reference
- 1. Soares-Bezerra RJ, Ferreira NCdS, Alberto AVP, Bonavita AG, Fidalgo-Neto AA, Calheiros AS, et al. (2015) An Improved Method for P2X7R Antagonist Screening. PLoS ONE 10(5): e0123089 doi:10.1371/journal.pone.0123089 [DOI] [PMC free article] [PubMed] [Google Scholar]