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. 2015 Mar;144(3):530–539. doi: 10.1111/imm.12403

Figure 2.

Figure 2

Human α1-antitrypsin (hAAT) alters β-cell-induced natural killer (NK) cell activation. (a–c) Islets were isolated from mice treated with hAAT at the indicated doses or with PBS (n = 4 per group). Twenty dissociated islets per sample were incubated with Poly(I:C)-treated NK cells in the presence or absence of 0·5 mg/ml hAAT. CD107a+ NK cells out of NKp46+ cells. Data representative of four experimental repeats. (d) Islets were isolated from mice treated with hAAT (2 mg per animal) or PBS (n = 3 per group). Twenty dissociated islets per sample in duplicate were incubated with Poly(I:C)-treated NK cells in the presence or absence of 0·5 mg/ml hAAT. Where stated, anti-NKp46 agonist antibody-coated wells were used. Interferon-γ (IFN-γ) release was measured after 48 hr. Data representative of two experimental repeats. Mean ± SEM, *P < 0·05, **P < 0·01.