Table 1. Methods used to test for KRAS, NRAS and BRAF mutations.

	KRAS					NRAS					BRAF
Method*	codon 12/13 N (%)	codon 59 N(%)	codon 61 N (%)	codon 117 N (%)	codon 146 N(%)	codon 12/13 N (%)	codon 59 N (%)	codon 61 N(%)	codon 117 N (%)	codon 146 N (%)	codon 600 N (%)
Next generation sequencing
Illumina MiSeq Oncopanet (Illumina)[25]	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	0 (0,0)	0 (0,0)	10 (5,8)
IonTorrent (Life Sciences)	20 (11,7)	20 (11,7)	20 (11,7)	20 (11,7)	20 (11,7)	20 (11,7)	20 (11,7)	20 (11,7)	10 (5,8)	20 (11,7)	20 (11,7)
Sequencing
Sanger Sequencing	51 (29,8)	61 (35,7)	61 (35,7)	73 (42,7)	84 (19,2)	74 (43,3)	84 (49,2)	74 (434)	83 (48,6)	73 (487)	51 (29,8)
HRM + Sanger Sequencing	60 (35.1)	60 (15.1)	60 (35.1)	30 (17.5)	30 (17.5)	.30 117.5)	30 (17.5)	30 (17.5)	0 (0.0)	0 (0.0)	40 (23.4)
Real-time PCR + Sanger sequencing	10 (5,8)	0 (0,0)	0 (0,0)	0 (0,0)	0 (0,0)	10 (5,8)	0 (0,0)	10 (5,8)	0 (0,0)	0 (0,0)	10 (5,8)
Pyrosequencing	0 (0,0)	0 (0,0)	0 (0,0)	0 (0,0)	0 (0,0)	0 (0,0)	0 (0,0)	0 (0,0)	10 (5,8)	10 (5,8)	0 (0,0)
Other assays
Therascreen KRAS/NRAS/BRAF Pyrokit (Qiagen)	10 (5,8)	0 (0,0)	10 (5,8)	0 (0,0)	0 (0,0)	10 (5,8)	0 (0,0)	10 (5,8)	0 (0,0)	0 (0,0)	10 (5,8)
Therascreen RAS extension Pyrokit (Qiagen)	0 (0,0)	10 (5,8)	0 (0,0)	10 (5,8)	10 (5,8)	0 (0,0)	10 (5,8)	0 (0,0)	10 (5,8)	10 (5,8)	-
Sequenom MassArray (Sequenom)	10 (5,8)	0 (0,0)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)	10 (5,8)
Total samples tested	171 (100)	161 (94,2)	171 (100)	153 (89,5)	161 (95,9)	164 (95,9)	164 (95,9)	161 (95,9)	123 (71,9)	123 (71,9)	151 (88,3)*

^*Total 171 samples received from 17 labs, 10 samples per lab, 1 lab sent 11 DNA samples;

^*4 laboratories only test BRAF on request by the physician.

Note: for each codon. the most frequently used method is highlighted in bold