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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Jan 1;90(1):21–24. doi: 10.1073/pnas.90.1.21

Bidirectional regulation of Na+,K(+)-ATPase activity by dopamine and an alpha-adrenergic agonist.

F Ibarra 1, A Aperia 1, L B Svensson 1, A C Eklöf 1, P Greengard 1
PMCID: PMC45591  PMID: 7678337

Abstract

Catecholamines have pronounced effects on the renal handling of sodium and water, dopamine-promoting sodium and water excretion, and norepinephrine-promoting sodium and water retention. In the present study, using isolated permeabilized renal tubule cells and intact rats, we have shown that these effects can be attributed to opposing actions of these transmitters on renal tubular Na+,K(+)-ATPase activity. The ability of each of these catecholamines to regulate Na+,K(+)-ATPase activity is affected by the concentration of Na+ as well as by the absence or presence of the opposing catecholamine.

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Selected References

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  1. Aperia A., Bertorello A., Seri I. Dopamine causes inhibition of Na+-K+-ATPase activity in rat proximal convoluted tubule segments. Am J Physiol. 1987 Jan;252(1 Pt 2):F39–F45. doi: 10.1152/ajprenal.1987.252.1.F39. [DOI] [PubMed] [Google Scholar]
  2. Aperia A., Fryckstedt J., Svensson L., Hemmings H. C., Jr, Nairn A. C., Greengard P. Phosphorylated Mr 32,000 dopamine- and cAMP-regulated phosphoprotein inhibits Na+,K(+)-ATPase activity in renal tubule cells. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2798–2801. doi: 10.1073/pnas.88.7.2798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aperia A., Ibarra F., Svensson L. B., Klee C., Greengard P. Calcineurin mediates alpha-adrenergic stimulation of Na+,K(+)-ATPase activity in renal tubule cells. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7394–7397. doi: 10.1073/pnas.89.16.7394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bello-Reuss E., Higashi Y., Kaneda Y. Dopamine decreases fluid reabsorption in straight portions of rabbit proximal tubule. Am J Physiol. 1982 Jun;242(6):F634–F640. doi: 10.1152/ajprenal.1982.242.6.F634. [DOI] [PubMed] [Google Scholar]
  5. Bertorello A. M., Aperia A., Walaas S. I., Nairn A. C., Greengard P. Phosphorylation of the catalytic subunit of Na+,K(+)-ATPase inhibits the activity of the enzyme. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11359–11362. doi: 10.1073/pnas.88.24.11359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bertorello A., Aperia A. Inhibition of proximal tubule Na(+)-K(+)-ATPase activity requires simultaneous activation of DA1 and DA2 receptors. Am J Physiol. 1990 Dec;259(6 Pt 2):F924–F928. doi: 10.1152/ajprenal.1990.259.6.F924. [DOI] [PubMed] [Google Scholar]
  7. Bialojan C., Takai A. Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem J. 1988 Nov 15;256(1):283–290. doi: 10.1042/bj2560283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eklöf A. C., Hökflet T., Aperia A. Renal nerve activity does not contribute to the development of renovascular hypertension in rats with abdominal aortic constriction. Acta Physiol Scand. 1991 Jan;141(1):71–77. doi: 10.1111/j.1748-1716.1991.tb09046.x. [DOI] [PubMed] [Google Scholar]
  9. Guyton A. C. Blood pressure control--special role of the kidneys and body fluids. Science. 1991 Jun 28;252(5014):1813–1816. doi: 10.1126/science.2063193. [DOI] [PubMed] [Google Scholar]
  10. Halpain S., Girault J. A., Greengard P. Activation of NMDA receptors induces dephosphorylation of DARPP-32 in rat striatal slices. Nature. 1990 Jan 25;343(6256):369–372. doi: 10.1038/343369a0. [DOI] [PubMed] [Google Scholar]
  11. Hemmings H. C., Jr, Greengard P., Tung H. Y., Cohen P. DARPP-32, a dopamine-regulated neuronal phosphoprotein, is a potent inhibitor of protein phosphatase-1. Nature. 1984 Aug 9;310(5977):503–505. doi: 10.1038/310503a0. [DOI] [PubMed] [Google Scholar]
  12. Liu J., Albers M. W., Wandless T. J., Luan S., Alberg D. G., Belshaw P. J., Cohen P., MacKintosh C., Klee C. B., Schreiber S. L. Inhibition of T cell signaling by immunophilin-ligand complexes correlates with loss of calcineurin phosphatase activity. Biochemistry. 1992 Apr 28;31(16):3896–3901. doi: 10.1021/bi00131a002. [DOI] [PubMed] [Google Scholar]
  13. Maxwell M. H., Waks A. U. Cations and hypertension: sodium, potassium, calcium, and magnesium. Med Clin North Am. 1987 Sep;71(5):859–875. doi: 10.1016/s0025-7125(16)30813-6. [DOI] [PubMed] [Google Scholar]
  14. Roccella E. J., Bowler A. E., Horan M. Epidemiologic considerations in defining hypertension. Med Clin North Am. 1987 Sep;71(5):785–801. doi: 10.1016/s0025-7125(16)30808-2. [DOI] [PubMed] [Google Scholar]
  15. Shyjan A. W., Ceña V., Klein D. C., Levenson R. Differential expression and enzymatic properties of the Na+,K(+)-ATPase alpha 3 isoenzyme in rat pineal glands. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1178–1182. doi: 10.1073/pnas.87.3.1178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tamaoki T. Use and specificity of staurosporine, UCN-01, and calphostin C as protein kinase inhibitors. Methods Enzymol. 1991;201:340–347. doi: 10.1016/0076-6879(91)01030-6. [DOI] [PubMed] [Google Scholar]

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