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International Journal of Nanomedicine logoLink to International Journal of Nanomedicine
. 2015 Aug 28;10(Spec Iss):215–225. doi: 10.2147/IJN.S88311

Silica nanoparticle-based dual imaging colloidal hybrids: cancer cell imaging and biodistribution

Haisung Lee 1, Dongkyung Sung 2, Jinhoon Kim 3, Byung-Tae Kim 3, Tuntun Wang 4, Seong Soo A An 5, Soo-Won Seo 6,✉, Dong Kee Yi 4,✉
PMCID: PMC4559254  PMID: 26357472

Abstract

In this study, fluorescent dye-conjugated magnetic resonance (MR) imaging agents were investigated in T mode. Gadolinium-conjugated silica nanoparticles were successfully synthesized for both MR imaging and fluorescence diagnostics. Polyamine and polycarboxyl functional groups were modified chemically on the surface of the silica nanoparticles for efficient conjugation of gadolinium ions. The derived gadolinium-conjugated silica nanoparticles were investigated by zeta potential analysis, transmission electron microscopy, inductively coupled plasma mass spectrometry, and energy dispersive x-ray spectroscopy. MR equipment was used to investigate their use as contrast-enhancing agents in T1 mode under a 9.4 T magnetic field. In addition, we tracked the distribution of the gadolinium-conjugated nanoparticles in both lung cancer cells and organs in mice.

Keywords: dual bioimaging, MR imaging, silica colloid, T1 contrast imaging, nanohybrid

Introduction

Generated molecular images can reflect the molecular and metabolic pathways within cells. In particular, tracking biological progress in a physiological environment and detecting possible malfunctions has been advocated as a means of diagnosing disease in its early stages. One commercially important magnetic resonance (MR) enhancing material is tetra-azacyclododecane-tetraacetic acid (TTA) containing gadolinium (Gd).1 Moieties of TTA become paramagnetic by the interaction between the d-orbital of Gd and lone pairs of electrons in nitrogen. The carboxylic ions attached to a macrocyclic ligand can also interact with the Gd ions. It is important that these molecular imaging probes can selectively target specific cells or organs for accurate diagnosis and treatment. Surface-modified silica nanoparticles afford the feasibility of combining various functionalities onto the silica surface.2–21 Moreover, they have good water wettability and low cytotoxicity for in vivo application.22 Cellular imaging agents must produce adequate real-time diagnostic images.23 Real-time dual mode analysis and cell tracking protocols have been studied.24–30 Ligand-decorated silica particles have been widely used as analytical and biosensing tools.31–40 Timely multi-functional MR imaging contrast-enhancing nanoparticles have been studied in biomedical applications.41–52

With the above in mind, we designed silica nanoparticles to have a dual imaging mode, ie, (MR) imaging and fluorescent optical imaging. Poly(ethylene glycol) (PEG) and PEG-containing block copolymers, with very flexible and hydrophilic properties, were conjugated onto the silica nanoparticles to enable them to escape uptake by the mononuclear phagocyte system. Qianjun et al reported that PEGylated nanoparticles reduce nonspecific binding of serum proteins and cellular responses.53 The silica nanoparticles were decorated further with Gd ions and fluorescent dye. The resulting functionalized silica (hereafter referred to as dual imaging silica) nanoparticles were characterized by transmission electron microscopy (TEM), energy-dispersive spectroscopy, dynamic light scattering (DLS), and optical imaging analysis. The particles were further investigated as potential MR imaging agents when studying T1 relaxivity and image-enhancing efficiency. Transfection of lung cells was implemented using lung cancer cells and the corresponding organ cell has been imaged under the multi-modal imaging such as fluorescence and magnetic resonance imaging. The distribution of these dual imaging silica nanoparticles to the organs after intraperitoneal injection was studied in a mouse model by elemental analysis using inductively coupled plasma mass spectrometry (ICPMS).

Materials and methods

Materials

Most of the materials used in this study were obtained from commercial sources. Trimethoxy(3-[oxiran-2-ylmethoxy] propyl)silane (TPS), branched polyethylenimine (PEI, molecular weight 25,000), 4′,6-diamidino-2-phenylindole (DAPI), NaHCO3, silica (LUDOX AS-40) nanoparticles, dichloromethane (DCM), GdCl3·6H2O, dimethyl sulfoxide, sodium sulfate (Na2SO4), sulfonic acid sodium salt 97%, ethyl acetate, rhodamine isothiocyanate (RITC), and hexane were obtained from Sigma-Aldrich. Mono-N-hydroxysuccinate (NHS)-PEG (molecular weight 5,000) was purchased from Sunbio Chem (Korea).

Fabrication of dual imaging silica nanoparticles

Branching of mono-NHS-PEG and PEI

A solution of mono-NHS-PEG (50 mg, 10 mmol) in anhydrous DCM (80 mL) was added to a solution of PEI (250 mg, 10 mmol) in anhydrous DCM (80 mL) with cooling in an ice bath. The mixture was stirred at room temperature and the temperature was allowed to increase naturally from 0°C for 12 hours. Thin layer chromatography with ethyl acetate and hexane at a ratio of 1:1 (v/v) was used to check the progress of the reaction. The solvent was evaporated by speed-vacuum and freeze-drying and the crude compound was kept in a freezer at −20°C. The crude solid material obtained after evaporation of the solvent was then purified by gel permeation chromatography (Sephadex LH20, CH2Cl2).

Branching of PEG-PEI and TPS

A solution of PEG-PEI (100 mg, 3.3 mmol) in DCM (80 mL) was added to a solution of TPS (0.80 mL, 4.6 mmol) in the same solvent (20 mL) with cooling in an ice bath. The mixture was stirred at room temperature; thereafter, the temperature was allowed to increase naturally from 0°C for 12 hours.

Synthesis of PEI-PEG silica nanoparticles

PEI-PEG (53 mg, 0.13 mmol) and TPS were placed in a round bottomed flask and dissolved with 1.25 mL of ethanol, 1.25 mL of water, and 1.25 mL of acetic acid. An aqueous suspension (125 µL) of silica (LUDOX AS-40) nanoparticles (Sigma-Aldrich) was added. The reaction mixture was warmed to 80°C under stirring for 24 hours. The ethanol was evaporated under reduced pressure, and solid NaHCO3 was added to the suspension to reach a pH 7–8. The precipitate was filtered and washed with a borate buffer solution (pH 9.5, 5×2 mL) and with water (5×2 mL). The solid was dried under vacuum and then redissolved in 100 mL of DCM. The organic solution was washed with water (100 mL) and dried over Na2SO4.

Synthesis of RITC-labeled PEI-PEG silica nanoparticles

A colloidal dispersion of PEI-PEG silica nanoparticles (100 µg/mL) was placed in a scintillation tube, and 5 mmol RITC in dimethyl sulfoxide was added (pH 7.7±0.3). The mixture was agitated for 12 hours at room temperature, and then cleaned in DCM. Finally, dialysis was carried out using a dialysis tube for 48 hours (molecular weight cut-off, 50 kDa).

Synthesis of Gd (III)-PEI-PEG-RITC silica nanoparticles

RITC-labeled PEI-PEG silica nanoparticles (5 mg) were placed in a round bottomed flask and deionized water was added to create a neutral pH condition. Additionally, a GdCl3 (10 mM) solution was provided and vortexed for 12 hours at 90°C. Arsenazo (III) solution was used with a colorimetric method to investigate binding between the Gd (III) ions and the PEI-PEG-RITC silica nanoparticles. The color of Arsenazo (III) solution changes to green when free Gd (III) ion are present in solution; further, the bright pink color of Arsenazo III solution changes to dark purple when Gd (III) ion become conjugated with certain ligands.

Quantitative analysis of Gd (III) ions by ICPMS

To verify the concentration of Gd in the purified complexes, ICPMS was performed using a computer-controlled Agilent 7500 device. Samples were prepared by digestion in nitric acid (ratio of nitric acid to sample, 9:1) in a 70°C water bath. The samples were diluted in 15 mL conical vials with a final concentration of 3% (v/v) nitric acid. Gd (III) standards were purchased from AccuStandard (New Haven, CT, USA) and diluted to 0.1, 1, 5, 10, and 50 ng/mL of Gd (III). The samples were treated with 60% HNO3 (1 mL) and 35% HCl (3 mL), and then pre-processed and analyzed with ICPMS (300 W power, 130 psi pressure, and 70°C temperature).

Dynamic light scattering

The complexes were investigated by DLS for particle size and zeta potential using a Nano ZS instrument at 25°C. An He-Ne laser producing vertically polarized light (λ0 approximately 632.8 nm) was used as the light source. Each sample was filtered using a 0.2 µm cellulose acetate membrane.

Cell culture and transfection of PEI-PEG silica nanoparticles and RNA

A549 (human alveolar basal epithelial) cells were sourced from the Korea Cell Line Bank (Seoul, Republic of Korea) and cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% antibiotics (Invitrogen, Grand Island, NY, USA) at 37°C in a humidified atmosphere of 5% CO2. The cells were seeded in a 12-well culture plate at a density of 2×105 cells per well and allowed to attach overnight. The incubation medium was then replaced by fresh medium. Dual imaging silica nanoparticles of different concentrations (0.1–1 mg/mL) were transferred into the wells by dropwise. After incubation for 4 hours, the old medium was replaced with fresh medium, and the nanoparticle-treated cells were incubated for 24 hours. The nucleus was stained with DAPI (blue) and the complex was represented with red for successful transfection of Gd(III)-PEI-PEG-RITC-silica nanoparticles. Since A549 cell, human derived neuroblastoma cell, was commercially available, ethics was not sought.

Animal experiments and animal care

Normal SD mouse (20 g weight) was used and animal number was two (Central Lab Animal Inc, Seoul, Korea). First, 100 µL of the dual imaging silica nanoparticle solution was injected slowly using a 1 mL syringe into the tail vein. Concentration of the dual imaging silica nanoparticle colloidal solution 100 µL (2 mg/mL) was injected into the mice. The dual imaging silica nanoparticles were administered by intraperitoneal injection. The mice were euthanized using CO2 gas in the euthanasia chamber supplied by compressed gas cylinders. One hour later, the internal organs, including kidney, spleen, pancreas, heart, lungs, and liver, were removed from each animal. The organs were then fixed in 10% formalin solution and investigated for distribution of Gd by ICPMS.

All animals were housed and maintained at 20°C–24°C on a 50:50 light-dark cycle. They received humane care in compliance with the Guide for the Care and Use of Laboratory Animals prepared by the Institute of Laboratory Animal Resources and published by the US National Institutes of Health and in accordance with the animal experiment guidelines of Samsung Biomedical Research Institute (SBRI). The study was reviewed and approved by the Institutional Animal Care and Use Committee of the SBRI. SBRI is a facility accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International and abides by the Institute of Laboratory Animal Resources guidelines.

Results and discussion

The entire synthesis procedure is summarized in Figure 1. Branched PEI was PEGylated using amine and NHS conjugation chemistry. PEGylated PEI was conjugated onto silica nanoparticles via TPS. The PEI-PEG silica nanoparticles were investigated by solid-state nuclear magnetic resonance (NMR) (Figure S1). The particles were reacted further with RITC dye, and Gd was conjugated onto the particles via the polyamine group on the PEI (Figure 1). The morphological characteristics of the dual imaging silica nanoparticles were investigated by both DLS and TEM. The average particle size was 40±7 nm by DLS (Figure 2A) and TEM (Figure 2B). Moreover, elemental analysis by energy-dispersive spectroscopy (interfaced with TEM) showed that weight portion of 64Gd was approximately 4.92% and that of 29Si and 8O was 48.85% and 46.23%, respectively (Figure 2C–E).

Figure 1.

Figure 1

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Synthesis of dual imaging silica nanoparticles.

Abbreviations: Gd, gadolinium; NHS, N-hydroxysuccinate; PEG, poly(ethylene glycol); PEI, polyethylenimine; TPS, trimethoxy(3-[oxiran-2-ylmethoxy]propyl)silane.

Figure 2.

Figure 2

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(A) Particle size distribution (by dynamic light scattering) and (B) morphological analysis (by transmission electron microscopy) of Gd silica nanoparticles, (C) energy-dispersive spectroscopic elemental analysis, and the corresponding elemental mapping of Si (D) and Gd (E).

Abbreviations: Si, silica; Gd, gadolinium.

The hydrophilic properties of silica nanoparticles and the multiple amine functionality grafts on the PEGylated PEI create an environment appropriate for enhancing MR imaging in T1 mode.54 The hydrophobic surface moiety on the dual imaging silica could lead to it being captured and anchored in the liver, while the hydrophilic polar coating by which surface charges55 can prolong its retention in the circulation.56–59 Further, the surface amine groups on the dual imaging silica can chelate Gd ions and interact in an aqueous environment to produce T1-weighted MR images, with hydration on the dual imaging silica nanoparticles affecting the image contrast. It has been reported that relaxivity is dependent on the hydration environment of Gd conjugate’s its number.59

Figure 3 shows that the dual imaging silica nanoparticles upon transfer into A549 (human lung cancer) cells. The nanoparticles were prepared at different concentrations (0.1–1 mg/mL) and transfected into the wells dropwise. After incubation for 4 hours, the old medium was replaced by fresh medium, and the nanoparticle-treated cells were incubated for 24 hours. The nucleus was stained with DAPI (blue), and the dual imaging silica nanoparticles showed red fluorescence (Figure 3A–C). Figure 3D shows the T1-weighted MR imaging for the colloidal silica solution produced by a microimaging analyzer (9.4 T). In addition, the dual imaging silica nanoparticles had red fluorescence functionality (Figure 3E). Atul et al have reported that the surface charge on nanoparticles can affect cellular localization, and shown that positively charged nanoparticles demonstrate significant uptake in A549 lung cancer cells when compared with negatively charged nanoparticles.60 Our dual imaging silica nanoparticles had a strong positive charge (+50 mV, Figure S2). In agreement with Atul et al our positively charged dual imaging silica nanoparticles were easily transfected into A549 lung cancer cells.

Figure 3.

Figure 3

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(A–C) Feasibility of cell transfection in A549 lung cancer cells in fluorescent imaging mode using gadolinium-conjugated silica nanoparticles (0.2 mg/mL).

Notes: (A) Nuclei stained by 4,6-diamidino-2-phenylindole (blue), (B) dual imaging silica nanoparticles represents red fluorescence (rhodamine isothiocyanate dye). (C) Merged fluorescence microscopic image of dual imaging silica nanoparticles transfected in A549 lung cancer cells. Gadolinium-conjugated silica nanoparticles in colloidal solution using T1-weighted magnetic resonance imaging (D) and fluorescent imaging mode (E).

The longitudinal (r1) MR relaxivity for dual imaging silica nanoparticles was measured (Figure 4A). In addition, each T1 relaxation time is shown in Figure 4B. High-resolution MR imaging was carried out using a Bruker vertical spectrometer (wide-bore, 9.4 T, 400.2 MHz). Gradient echo sequences were used to acquire the MR images (effective spectral bandwidth 101010.1 Hz, echo time 3.000 milliseconds, echo position 50%, repetition time 100.000 milliseconds) in order to compare all the samples (with a fixed receiver gain value of 370.0). A 128×128 acquisition matrix was used for a field of view. The total scan time measured was 125,800 milliseconds in one accumulation experiment. For the T1 measurements, the echo time was regulated at 79.225, 125, 250, 500, 750, 1,000, 2,000, and 3,000 milliseconds. The echo time was 3.640 milliseconds. The field of view was the same as on the two-dimensional image. In the estimated total scan time, it has taken 0 hour 16 minutes 26 seconds 140 milliseconds. The dwell time was 0.004950 milliseconds and the minimum repetition time was 16.685 milliseconds. Relaxivity measurements were acquired by taking the slope of a plot of 1/T1 (s−1) versus concentration (mM). The longitudinal water proton relaxation times (T1) were determined using 400 MHz solid-state NMR with a Bruker micro-imaging probe analyzer operating at 400 MHz and 25°C.

Figure 4.

Figure 4

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(A, B) Relaxation (r1) rates with different concentrations of dual imaging silica nanoparticles and (C, D) T1-weighted images using a 9.4 T micro-imaging analyzer instrument. Colloidal solution of dual imaging silica nanoparticles in water (C) and a pure water sample (D).

Interestingly, the relaxivity was higher (r1=9.41±0.32 mM−1 s−1) when compared with a previous report for a T1 Gd agent (r1=3 mM−1 s−1).61 We used ICPMS to determine our Gd ion concentration, and T1 relaxivity was measured and compared using four different concentrations. Two-dimensional images were obtained using dual imaging silica nanoparticle dispersion (Figure 4C) and pure deionized water (Figure 4D) for comparison. Significant signal enhancement in the T1-weighted image was observed for the dual imaging silica nanoparticle dispersion. We believe that the highly hydrophilic environment of the dual imaging silica nanoparticles allows for easy access of water molecules to the Gd (III) ion for water proton relaxation.62–65 Kim et al reported on the use of Gd-TSPETE-silica nanoparticle-[Ru(bpy)3]2+ (r1 =9 mM−1 s−1).64 Therefore, the r1 relaxivity value for our Gd-PEI-PEG silica nanoparticles is similar to that of other silica nanoparticle-based MR imaging agents.

Information on the in vivo distribution of dual mode nanoparticles is useful for bioimaging purposes. Figure 5 compares the biodistribution imaging results at 1 hour and 6 hours after intraperitoneal injection of the nanoparticles. One hour after injection, rich storage was not observed in the pancreas; however, 6 hours after injection, Gd ions were accumulated primarily in the pancreas rather than in other organs such as liver, kidney, lung, pancreas, and spleen. Although Gd enrichment was observed in pancreas, broad distribution in all organs was clear. The circulation time and activity of nanoparticles in blood vessels can be enhanced by surface PEGylation. Our dual imaging silica nanoparticles utilized a PEGylation strategy and consequently showed broad biodistribution in all organs in a mouse model. We believe that the enhanced hydrophilicity of the PEGylated surface on the dual imaging silica nanoparticles increased the particle circulation time. Consequently, broad distribution of Gd was achieved in vivo.55–58

Figure 5.

Figure 5

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Distribution of gadolinium to the organs in an in vivo mouse model. The upper panel shows organ-dependent inductively coupled plasma mass spectrometry data 6 hours after injection. The lower panel shows the corresponding data at 1 hour and 6 hours after injection.

Abbreviations: IP, Intraperitoneal; Gd, gadolinium.

Conclusion

In summary, we used covalent bonding to construct nano-particles with both MR imaging and fluorescence function-alities. Our Gd-decorated hybrid silica nanoparticles had highly enhanced MR relaxivity. In addition, optical imaging by fluorescence was achieved due to the surface-layered dyes. The polyamine and polycarboxyl groups increased the chelation efficiency of these Gd-silica nanoparticles. We believe that this protocol can be used to develop other useful bioimaging and theranostic silica hybrids for in vivo and in vitro biomedical applications. In the near future, we will report on our in vivo imaging research efforts to enhance the targeting ability of these conjugated hybrid silica nanoparticles.

Supplementary materials

In order to provide more detailed characterization, solid-state nuclear magnetic resonance (NMR) analyses were performed, 600 MHz 1H-NMR spectrum and 400 MHz 29Si, 13C CP MAS NMR spectrum. (A) 29Si CP MAS NMR spectrum of aminopropyl-functionalized silica nanoparticles and PEI 25,000-PEG 5,000 graft silica nanoparticles were compared with silica nanoparticles. (B) 13C CP MAS NMR spectrum of aminopropyl-functionalized silica nanoparticles and PEI 25,000-PEG 5,000 graft silica nanoparticles were also compared with silica nanoparticles. (C) 600 MHz 1H-NMR NMR spectra are shown for PEI 25,000, PEG 5,000, and PEI 25,000-PEG 5,000 copolymer.

Solid-state 29Si CP-MAS NMR analyses were performed using a Bruker Avance 400 MHz spectrometer operating at 79.54 5 MHz. The samples were located in 4 mm ZrO2 rotors. 7 kHz. The spectrum width was 23.8 kHz, and the delay or repetition time was 5.0 seconds. Contact time was performed for 5 milliseconds. The 29Si spectrum was calibrated with the signal obtained using the external standard DSS [3-(trimethylsilyl)-1-propane sulfonic acid, sodium salt 97%, Sigma Chemicals] at 0.00 ppm.

The 13C solid-state NMR experiments were performed with the same NMR instrument, ie, 29Si CP-MAS NMR operating with 100.690 MHz. ZrO2 rotors (4 mm) were used, and magic angle spinning was carried out at a spinning rate of 7 kHz, a 30.3 kHz spectrum width, a 5.0 seconds delay or repetition time, and a 2 milliseconds contact time.

Figure S1

Solid-state NMR analysis of 400 MHz 29Si, 13C CP MAS NMR spectrum. 29Si CP MAS NMR spectrum and 600 MHz 1H-NMR spectrum.

Notes: 29Si CP MAS NMR spectrum of aminopropyl functionalized silica nanoparticles and PEI 25-PEG 5k graft silica nanoparticles as well as silica nanoparticles (A), 13C CP MAS NMR spectrum of aminopropyl functionalized silica nanoparticles and PEI 25-PEG 5k graft silica nanoparticles as well as silica nanoparticles (B), 600 MHz 1H-NMR NMR spectrum of PEI 25k, PEG5k as well as PEI 25k-PEG 5k copolymer (C).

Abbreviations: APTES, (3-aminopropyl)triethoxysilane; NP, nanoparticle; PEG, poly(ethylene glycol); PEI, polyethylenimine; ppm, parts per million, NMR, nuclear magnetic resonance.

ijn-10-215s1.tif (256.2KB, tif)
Figure S2

Zeta potential of the dual imaging silica nanoparticles.

ijn-10-215s2.tif (198.8KB, tif)

Acknowledgments

This work was supported by the 2015 Myongji University Research Fund.

Footnotes

Disclosure

The authors report no conflicts of interest in this work.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Figure S1

Solid-state NMR analysis of 400 MHz 29Si, 13C CP MAS NMR spectrum. 29Si CP MAS NMR spectrum and 600 MHz 1H-NMR spectrum.

Notes: 29Si CP MAS NMR spectrum of aminopropyl functionalized silica nanoparticles and PEI 25-PEG 5k graft silica nanoparticles as well as silica nanoparticles (A), 13C CP MAS NMR spectrum of aminopropyl functionalized silica nanoparticles and PEI 25-PEG 5k graft silica nanoparticles as well as silica nanoparticles (B), 600 MHz 1H-NMR NMR spectrum of PEI 25k, PEG5k as well as PEI 25k-PEG 5k copolymer (C).

Abbreviations: APTES, (3-aminopropyl)triethoxysilane; NP, nanoparticle; PEG, poly(ethylene glycol); PEI, polyethylenimine; ppm, parts per million, NMR, nuclear magnetic resonance.

ijn-10-215s1.tif (256.2KB, tif)
Figure S2

Zeta potential of the dual imaging silica nanoparticles.

ijn-10-215s2.tif (198.8KB, tif)

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