Figure. 3.

Detection of Cre activity in Shox2^cre/+;R26R double heterozygous embryos. (A) Cre-mediated beta-galactosidase expression (blue) in the sinus venosus (black arrow) was detectable at E9.5. (B) At E10.5, beta-galactosidase expression in Shox2^cre/+;R26R double heterozygous embryos persisted in the sinus venosus (SV), and was also found in the proximal domain of the limb buds, the dorsal root ganglion (white arrowheads), and the temporomandibular junction (white arrow). (C) An E11.5 Shox2^cre/+;R26R embryo showing beta-galactosidase activity in the same organs and tissues as described in E10.5 embryo. (D) X-gal staining was seen in the SAN of an E11.5 Shox2^cre/+;R26R embryo. (E) Beta-galactosidase expression was detected in the anterior domain (black arrowheads) of the palatal shelves (PS). (F) Beta-galactosidase expression was found restrictedly in the proximal region of the limb bud at E11.5.