Skip to main content
PMC home page
Cell Death and Differentiation logoLink to Cell Death and Differentiation
. 2015 Mar 6;22(10):1676–1686. doi: 10.1038/cdd.2015.16

Neuronal NLRP1 inflammasome activation of Caspase-1 coordinately regulates inflammatory interleukin-1-beta production and axonal degeneration-associated Caspase-6 activation

V Kaushal 1,2, R Dye 3, P Pakavathkumar 1,2, B Foveau 1,2, J Flores 1,2, B Hyman 4, B Ghetti 5, B H Koller 3, A C LeBlanc 1,2,*
PMCID: PMC4563782  PMID: 25744023

Abstract

Neuronal active Caspase-6 (Casp6) is associated with Alzheimer disease (AD), cognitive impairment, and axonal degeneration. Caspase-1 (Casp1) can activate Casp6 but the expression and functionality of Casp1-activating inflammasomes has not been well-defined in human neurons. Here, we show that primary cultures of human CNS neurons expressed functional Nod-like receptor protein 1 (NLRP1), absent in melanoma 2, and ICE protease activating factor, but not the NLRP3, inflammasome receptor components. NLRP1 neutralizing antibodies in a cell-free system, and NLRP1 siRNAs in neurons hampered stress-induced Casp1 activation. NLRP1 and Casp1 siRNAs also abolished stress-induced Casp6 activation in neurons. The functionality of the NLRP1 inflammasome in serum-deprived neurons was also demonstrated by NLRP1 siRNA-mediated inhibition of speck formation of the apoptosis-associated speck-like protein containing a caspase recruitment domain conjugated to green fluorescent protein. These results indicated a novel stress-induced intraneuronal NLRP1/Casp1/Casp6 pathway. Lipopolysaccharide induced Casp1 and Casp6 activation in wild-type mice brain cortex, but not in that of Nlrp1−/− and Casp1−/− mice. NLRP1 immunopositive neurons were increased 25- to 30-fold in AD brains compared with non-AD brains. NLRP1 immunoreactivity in these neurons co-localized with Casp6 activity. Furthermore, the NLRP1/Casp1/Casp6 pathway increased amyloid beta peptide 42 ratio in serum-deprived neurons. Therefore, CNS human neurons express functional NLRP1 inflammasomes, which activate Casp1 and subsequently Casp6, thus revealing a fundamental mechanism linking intraneuronal inflammasome activation to Casp1-generated interleukin-1-β-mediated neuroinflammation and Casp6-mediated axonal degeneration.

The lack of efficient treatment for Alzheimer disease (AD) is of high social and economical cost and a growing concern with the aging of the world's population.1 Therapies eliminating amyloid beta peptide (Aβ) from AD brains have unfortunately failed to stem progressive cognitive decline. These disappointing results have forced scientists to reconsider treatments against AD; some focusing on targeting Aβ earlier in disease, while others attempting to disaggregate the Tau protein in neurofibrillary tangles (NFT). Recently, the association of several immune responsive genes with increased AD risks2, 3, 4 have additionally revived interest in a possible etiological role for inflammation in AD.

AD brain inflammation is attributed to activated microglia, which remove Aβ, and secrete neurotoxic molecules that induce neurodegeneration. Interleukin-1-beta (IL-1β), a critical component of brain neuroinflammation, is increased in AD brains5 and may contribute to AD pathology by increasing amyloid precursor protein (APP) gene expression, Tau hyperphosphorylation and memory impairment.6 However, anti-inflammatory therapies have not provided the expected beneficial effect in AD patients,7 suggesting that microglial inflammation may be a consequence of AD. Degenerating neurons are renowned initiators of brain inflammatory responses and the loss of synapses remains the best correlative marker of dementia in AD.8 This has incited us to study the response of human neurons to stress and to determine whether specific neuronal molecular events were initiated that link axonal degeneration to an inflammatory response.

The active cysteinal Caspase-6 protease (Casp6), associated with axonal degeneration,9, 10, 11, 12, 13 is highly abundant in NFT, neuropil threads, and neuritic plaques of AD brains.14 In some aged non-cognitively impaired individuals, Casp6 activity in the entorhinal cortex and CA1 regions of the hippocampus,15 two areas initially affected by NFT pathology in AD,16 correlates significantly with lower cognitive performance.17 The expression of active Casp6 in CA1 pyramidal neurons of mouse brains is sufficient to induce age-dependent cognitive impairment, in the absence of plaques and tangles, which suggests that active Casp6 in AD brains could be a major contributor to axonal degeneration and cognitive decline.18

Despite substantial evidence implicating Casp6 in AD, the pathways leading to Casp6 activation in neurons are unclear. Caspase-1 (Casp1) activates Casp6 in primary cultures of human CNS neurons.19 Inflammasome multiprotein complexes, constituted of danger sensing nucleotide-binding oligomerization domain-like receptors or the DNA sensing absent in melanoma 2 (AIM2) component, and the apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), recruit and induce Casp1 self-activation.20, 21 Functional Nod-like receptor protein 1 (NLRP1), Nod-like receptor protein 3 (NLRP3), AIM2, and ICE protease activating factor (IPAF-1) inflammasomes have been characterized primarily in peripheral macrophages22 and CNS microglia.23, 24 Recently, reports have indicated inflammasome receptor expression and activation in rodent neurons. Rat cerebellar granule neurons submitted to oxygen and glucose deprivation or reduced potassium levels increased Nlrp1 mRNA levels.25, 26 Nuclear Nlrp1 or functional Nlrp1 inflammasome complexes increased in rat cortical neurons after traumatic brain injury, stroke, and glucose-oxygen deprivation insults.27, 28, 29, 30, 31 Neuronal Nlrp1 increased in rats submitted to spinal cord or sciatic nerve injury,29, 32 and in aging rat hippocampus or ethanol treated hippocampal slice cultures.33, 34 Aim2 induced pyroptosis in rat cortical neuron cultures and traumatic brain injury.35 Nlrp1 has been reported in human brain pyramidal neurons36 and inflammasome receptor mRNAs were observed in human neuron cultures and human Rasmussen's encephalitis.37

Here, we assessed which inflammasome could activate Casp1 and subsequently Casp6 in human primary CNS cultures. We determined which inflammasomes were expressed in naive and stressed neurons and used siRNAs and S-100 cell-free extracts treated with specific inflammasome activators, or antibody blockers, to identify the functional inflammasome. We uncovered that the NLRP1, AIM2, and IPAF-1, but not the NLRP3, inflammasomes were expressed and functional in neurons and that the NLRP1 inflammasome was responsible for Casp1 and subsequently Casp6 activation in serum-deprived and benzylated ATP (BzATP)-stressed neurons. NLRP1 was co-localized with Casp6 activity, immunostained 25- to 30-fold more neurons in AD, and increased Aβ42 in serum-deprived neurons. The NLRP1–Casp1–Casp6 pathway was blocked in lipopolysaccharide (LPS)-treated Nlrp1−/− and Casp1−/− mice brains. These results reveal a molecular cascade linking neuronal inflammasome-mediated Casp1 activation to Casp6 activation and provide unexpected novel common neuronal therapeutic targets against neuroinflammation, axonal degeneration, and cognitive impairment in AD.

Results

NLRP1, IPAF-1, and AIM2 inflammasome receptors are expressed in primary fetal human neurons

NLRP1, IPAF-1, AIM2, Casp1, and ASC inflammasome component mRNAs were detected at variable levels in naive human primary cultures of neurons, astrocytes, and microglia by RT-PCR (Figure 1a). In contrast, NLRP3 was not detected in any of these cell types, despite detection in the human monocyte cell line THP-1.38 The purity of the culture was confirmed by RT-PCR with neuronal microtubule-associated protein 2 (MAP2), astrocyte glial fibrillary acidic protein (GFAP), and microglia cluster of differentiation 68 (CD68). Only neuron cultures showed a slight astrocyte contamination, as expected. Casp6 mRNA was observed in neurons, astrocytes, and THP-1 cells, but was undetectable in microglia. Quantitative assessment of inflammasome receptors with qRT-PCR showed higher levels of NLRP1 and AIM2 mRNAs in neurons than in astrocytes and microglia, while IPAF-1 levels were equivalent in all CNS cell types (Figure 1b). Consistently, NLRP1 and AIM2 proteins were higher in neurons than in astrocytes, whereas IPAF-1 protein was equivalent in these cells (Figures 1c and d). Although NLRP3 was not detected by RT-PCR in any of the cell types, treatment of microglia with 100 ng/ml LPS for 24 h, induced its expression as expected (Figure 1e). Human neurons, astrocytes, and microglia differentially express inflammasome components indicating that CNS cells could have a differential response to danger- or pathogen-associated molecular patterns (DAMPs and PAMPs).

Figure 1.

Figure 1

Open in a new tab

Expression of inflammasomes in human primary neurons. (a) Ethidium bromide agarose-stained gel of NLRP1, NLRP3, IPAF-1 and AIM2 inflammasome receptors, ASC, Casp1, Casp6, MAP2, GFAP, CD68, and GAPDH RT-PCR amplicons from human primary neurons, astrocytes, and microglia and the monocyte cell line, THP-1 as a positive control for all inflammasomes. (b) qRT-PCR of inflammasome amplicon levels corrected for GAPDH levels and expressed relative to THP-1 levels. Data represent the mean and S.E.M. of a minimum of three independent experiments. One-way ANOVA (P=0.0006 for NLRP1 and P=0.0271 for AIM2) followed by Tukey–Kramer post hoc analysis, *P<0.05, **P<0.01, ***P<0.001. (c) Western blot of inflammasome receptors in neurons, astrocytes, and THP-1 cellular protein extracts. (d) Densitometric analysis of the western blot results of c. Data represents the mean and S.E.M. of three independent experiments. (e) Ethidium bromide agarose gel of NLRP3 RT-PCR amplicon from untreated (control) or LPS-treated microglia

Human neurons express functional inflammasomes

Serum deprivation of human neuron cultures, a condition previously observed to increase Casp1 activity,19 increased NLRP1 and AIM2 mRNA levels but did not change IPAF-1 mRNA levels, whereas NLRP3 mRNA levels remained undetectable (Figures 2a and b). To assess inflammasomes functionality in neurons, a cell-free system consisting of isolated cytosol (S-100) from serum-deprived neurons, as previously described for THP-1 cells,38 was treated with NLRP1 agonist, muramyl dipeptide (MDP), AIM2 agonist polydAT, or IPAF-1 agonist recombinant flagellin and incubated at 30 °C for 1.5 h, and tested for Casp1 activation (Figure 2c). The incubated S-100 extracts untreated with inflammasome activators showed a 10-fold increase in Casp1 activity after incubation, consistent with inflammasome formation under these conditions. Likewise, non-serum-deprived neuron S-100 incubated at 30 °C produced detectable Casp1 YVADase activity (Supplementary Figure S1a). Targeting the inflammasomes with 50 ng/ml MDP, 10 μg/ml polydAT, or 10 ng/ml flagellin increased Casp1 YVADase activity five-fold relative to the incubated control S-100 extracts (Figure 2c). Casp1 activation was confirmed by increased IL-1β in the incubated S-100 extracts (Figure 2d). Therefore, functional inflammasomes are not limited to immune cells and glia, but are also present in neurons.

Figure 2.

Figure 2

Open in a new tab

Functional inflammasomes in serum-deprived human neurons. (a) Ethidium bromide agarose gels of the NLRP1, NLRP3, IPAF-1, AIM2, and GAPDH RT-PCR amplicons from untreated and serum-deprived neurons compared with THP-1 cells. (b) NLRP1, IPAF-1, and AIM2 mRNA levels quantified relative to GAPDH mRNA levels by qRT-PCR in untreated and serum-deprived neurons. Statistical significance was assessed with a paired t-test. *P<0.05, **P<0.01. (c and d) Casp1 YVADase activity (c) and IL-1β (d) in S-100 cytosolic fractions isolated from serum-deprived primary human neurons (control) incubated without (untreated at 1.5 h 30 ºC) or with MDP, polydAT, and flagellin for 1.5 h at 30 ºC. (c and d) One-way ANOVA followed by Tukey–Kramer post hoc analysis (*P<0.05, **P<0.01) compares control with incubated samples, #P<0.05 and ##P<0.01 compares untreated with treated samples. Data represent mean and S.E.M. from three to six independent experiments (b–d)

The NLRP1 inflammasome regulates neuronal Casp1 activation

Incubation of serum-deprived neuronal S-100 at 30 °C significantly increased Casp1 YVADase activity. Incubation of S-100 with anti-NLRP1 inflammasome receptor antibodies robustly reduced Casp1 YVADase activity (Figure 3a), while anti-AIM2 and anti-IPAF-1 antibodies did not, even after doubling their concentration (Supplementary Figure S1b). To assess if another type of cellular stress can induce neuronal inflammasome formation, neurons were treated with BzATP, known to activate the P2X7 receptor (P2X7R) and the NLRP1 inflammasome in rat neurons.31 Human neurons and microglia, but not astrocytes, expressed P2X7R mRNA (Supplementary Figure S2a). The P2X7R was functional in neurons as evidenced by YO-PRO-1 dye uptake into neurons treated with BzATP, and inhibition of the YO-PRO-1 dye uptake by P2X7R antagonist, Brilliant Blue G (BBG; Supplementary Figures S2b and S2c). Treatment of human neurons with BzATP induced a four-fold increase in NLRP1 mRNA levels, while IPAF-1 and AIM2 mRNA levels did not change (Figures 3b and c). Furthermore, neurons treated with siRNA against NLRP1, which strongly reduced NLRP1 mRNA, eliminated serum deprivation-induced YVADase activity (Figure 3d) and IL-1β (Figure 3e), validating NLRP1-mediated Casp1 activation. Similar results were achieved with BzATP-treated neurons but did not reach statistical significance. As serum-deprived (Supplementary Figure S3a) or BzATP-treated (Supplementary Figure S3b) pure astrocyte cultures did not activate Casp1, the NLRP1 increase must be neuronal. The functional activation of an NLRP1 inflammasome in serum-deprived neurons was confirmed with the ASC conjugated to green fluorescent protein (ASC-GFP). Normally present in the cytosol, ASC-GFP is detected as condensed specks in cells when it associates with inflammasomes. As expected, ASC-GFP was distributed in the cytosol of normal neurons, but condensed into a speck in serum-deprived neurons (Figure 3f). However, siRNAs to NLRP1 prevented speck formation of ASC-GFP in serum-deprived conditions (Figure 3g), whereas neither siRNAs against Casp1 or Casp6 prevented speck formation (Figure 3g). Together, these results indicate that the NLRP1 inflammasome regulates Casp1 activation in serum-deprived and BzATP-treated human neurons.

Figure 3.

Figure 3

Open in a new tab

The NLRP1 inflammasome activates Casp1 in serum-deprived and BzATP-treated human neurons. (a) Casp1 YVADase activity in S-100 extracts from neurons exposed to serum deprivation and treated with 1 μg/ml antibodies against IPAF-1, AIM2, NLRP1, or GAPDH. (b and c) Ethidium bromide agarose gel of RT-PCR amplicons (b) and qRT-PCR measurements (c) of NLRP1, NLRP3, IPAF-1, and AIM2 from untreated and 500 μM BzATP-treated neurons. Data represent the mean and S.E.M. of four independent neuron cell cultures. One-way ANOVA (P=0.0052) and Tukey–Kramer post hoc analysis (**P<0.01) compared with control, and #P<0.05 comparing NLRP1 with AIM2 and IPAF-1. (d) Casp1 YVADase activity in neurons treated with 1 μM scrambled or NLRP1 siRNA for 72 h followed by 30 min serum deprivation or 500 μM BzATP. Lower panel shows the effectiveness of the NLRP1 siRNAs knock down. Data represent mean and S.E.M. from four independent experiments. Repeated measures of ANOVA with a Tukey–Kramer post hoc analysis *P<0.05 comparing with control and #P<0.05 comparing pairs of scrambled versus NLRP1 siRNAs. (e) IL-1β levels from samples in d. ***P<0.001 compared with control and ###P<0.001 comparing siNLRP1 with scrambled siRNA. (f) ASC-GFP showing specks in serum-deprived primary human neurons. Bar represents 20 μm. (g) Quantitation of two individual preparations of human neurons. Statistics represent a multiple t-test compared with control. *P<0.05, **P<0.01

NLRP1-induced Casp1 activation is responsible for Casp6 activation in human neurons: the NLRP1–Casp1–Casp6 pathway

Casp1 YVADase activity occurred within 15–30 min followed closely by Casp6 VEIDase activity in serum-deprived (Supplementary Figure S3c) and BzATP-treated (Supplementary Figure S3d) neurons, while Casp3 DEVDase activity was observed only at 18–24 h. Caspase activities were not observed in serum-deprived or BzATP-treated astrocytes (Supplementary Figure S3a and S3b), confirming the caspase activity to be from neurons. Casp6 activation appeared Casp1-dependent as the Casp1 YVAD peptide inhibitor prevented Casp6 VEIDase activity (Figures 4a and d), tubulin cleaved by Casp6 (TubΔCasp6; Figures 4b and e), and IL-1β production (Figures 4c and f) in serum-deprived (Figures 4a) and BzATP- (Figures 4d) treated neurons. NLRP1- and Casp-1-dependent Casp6 VEIDase activation were confirmed with siRNAs against NLRP1 and Casp1, which prevented Casp6 VEIDase activity in serum-deprived neurons (Figure 4g). A similar trend was observed in BzATP-treated neurons but did not reach statistical significance. Knock down of both NLRP1 and Casp1 mRNAs with their respective siRNAs was confirmed (Figure 4h). Furthermore, serum-deprivation and BzATP-induced TubΔCasp6 levels were attenuated with NLRP1 and Casp1 siRNAs (Figure 4i). These results establish the NLRP1–Casp1–Casp6 pathway in human CNS neurons exposed to serum deprivation.

Figure 4.

Figure 4

Open in a new tab

NLRP1 and Casp1 activate Casp6 in serum-deprived and BzATP-treated neurons. Casp6 VEIDase activity (a and d), TubΔCasp6, full-length tubulin (FL Tub), β-actin western blots (b and e) and IL-1β production (c and f) in serum-deprived (a,b, and c) or BzATP-treated (d,e, and f) neurons in the absence (DMSO control) or presence of 5 μM Casp1 Z-YVAD-fmk. Data represent the mean and S.E.M. of three (a and c) and four (d and f) independent neuron cultures. One-way ANOVA (P=0.002 in a, P=0.004 in c, and P=0.02 in d and f) followed by a Tukey–Kramer post hoc analysis (*P<0.05 and **P<0.01) relative to untreated DMSO control. (g) Casp6 VEIDase activity in neurons treated with 1 μM scrambled siRNA or siRNAs against Casp1 or NLRP1 followed by normal culture conditions (control), serum deprivation or 500 μM BzATP for 1 h. Data represent the mean and S.E.M. of four independent experiments. Scrambled siRNA-treated control was arbitrarily placed at one and other values within each experiment expressed relative to the scrambled siRNA control levels. One-way ANOVA (P=0.0106) followed by a post hoc Tukey–Kramer confirms increased Casp6 activity in serum-deprived neurons (*P≤0.05) and return to normal in siNLRP1 and siCasp1 serum-deprived neurons. Independent unpaired t-test shows #P<0.05 between scrambled siRNA and siNLRP1 or siCasp1 in serum-deprived conditions, and between scrambled siRNA and siNLRP1 in BzATP-treated neurons. (h) RT-PCR of NLRP1 and CASP1 mRNA in siNLRP1 and siCASP1-treated neurons, respectively. (i) Western blot of samples in g with TubΔCasp6 and full-length tubulin antibodies

The NLRP1–Casp1–Casp6 pathway exists in live brains

To determine whether NLRP1 and Casp1 mediate Casp6 activation in vivo, Nlrp1−/−, Casp1−/−, or wild-type (WT) mice were subjected to a single-dose intraperitoneal (IP) injection of 5 mg/kg LPS for 6 h.39 IL-1β increased almost 10-fold in the brains of LPS-treated WT mice, but not in Nlrp1−/− and Casp1−/− LPS-treated mice (Figure 5a). Casp6 VEIDase activity increased only slightly in LPS-treated WT mice brain cortex but was significantly depressed in control and LPS-treated Nlrp1 null mice brains (Figure 5b). However, Casp6 VEIDase activity was not decreased in Casp1 null mice submitted to LPS. The VEIDase activity in these cortical extracts was likely due to the activity of another protease as the inhibition of Casp6 activity was confirmed by immunostaining against TubΔCasp6 (Figures 5c and d). The level of TubΔCasp6 did not change significantly in WT versus Nlrp1−/− or Casp1−/− control mouse brains. However, TubΔCasp6 levels increased significantly in LPS-treated WT mice versus control, and were significantly decreased in LPS-treated Nlrp1−/− or Casp1−/− mice brains, compared with the LPS-treated WT mice brains. TubΔCasp6 levels were also significantly lower in the LPS-treated Nlrp1−/− compared with control WT untreated brains. Casp1 cannot cleave Tubulin (Figure 5e) and no significant levels of Casp3 DEVDase activity were observed (Figure 5f). Together, these data confirm the presence of the NLRP1–Casp1–Casp6 pathway in vivo.

Figure 5.

Figure 5

Open in a new tab

LPS-treated NLRP1−/− and Casp1−/− mice have reduced Casp1 and Casp6 activities. (a) Casp1-mediated IL-1β production, (b), Casp6 VEIDase activity (c), TubΔCasp6 western blots, (d) TubΔCasp6 relative to FL tubulin densitometry, and (f) Casp3 DEVDase activity in proteins extracted from the cortex of LPS-injected wild-type, NLRP1 null, and Casp1 null mice. Data represent the mean and S.E.M. from eight wild-type, six NLRP1 null, and three Casp1 null mice for (a,b and f), and three different mice for (c and d). Statistics were performed using one-way ANOVA (P=0.0014 for a, P=0.002 for b, and P=0.0001 for d) followed by a Tukey–Kramer post hoc test: **P<0.01, ***P<0.001 compares with WT control, ###P<0.001 compares with WT LPS (a,b and d). (e) Western blots of full-length α-tubulin cleaved by Casp6 and Casp1

NLRP1 inflammasome receptor is increased in AD brains and neurons

NLRP1, IPAF-1, AIM2, ASC, Casp1, and Casp6 mRNAs were expressed in human brain cerebellum and cortex (Figure 6a). The increase in NLRP1 mRNA levels in human AD cortex, relative to normal control brains, suggests that the NLRP1 inflammasome could lead to Casp6 activation in AD (Figure 6b). ASC, Casp1, and Casp6 mRNA levels were slightly but not significantly increased in AD cortex (Figures 6c–e). As observed in human primary CNS cell cultures, NLRP3 mRNA was not detected in normal or AD brain cortex and cerebellum, despite being easily detected in THP-1 cells (Figures 6a and 4s). Immunostaining of hippocampal tissue sections revealed a robust increase of NLRP1 immunopositive cells compared with normal control subiculum (Figures 6f and g). NLRP1 immunopositive staining was increased 25- to 30-fold in sporadic and familial AD subiculum (Figure 6h). NLRP1 immunopositive cells were not co-stained by GFAP, thereby excluding the presence of NLRP1 in astrocytes (Figure 6i). However, NLRP1 immunopositive cells were stained with TauΔCasp6, thereby confirming these cells to be neurons, some of which also contained high levels of active Casp6 (Figure 6j). Together, these data suggest that the NLRP1–Casp1–Casp6 pathway exists in human AD neurons and indicate a plausible role for NLRP1 in Casp6 activation.

Figure 6.

Figure 6

Open in a new tab

NLRP1 expression is increased in AD. (a) Ethidium bromide-stained agarose gel of NLRP1, NLRP3, IPAF-1, and AIM2 inflammasome receptors, ASC, CASP1, CASP6, and GAPDH RT-PCR amplicons from human adult cortex and cerebellum tissues, THP-1 cells, or water control. (b–d) qRT-PCR quantitation of cortex or cerebellar NLRP1 (b), ASC (c), CASP1 (d), and CASP6 (e) mRNAs from AD and healthy control individuals. Data represent mean and S.E.M. from 12 AD and 4 control cases. Statistical difference was assessed by an unpaired t-test with Welch correction (*P<0.05, ***P<0.001). (f and g) Low (f) and high (g) magnification micrographs of NLRP1 immunohistochemical staining in AD and non-AD subiculum. Scale bar is 100 μm in f and 50 μm in g and h. Density of NLRP1 immunopositive staining represented as μm2 positive staining per mm2 of tissue in non-AD, sporadic AD (SAD), and familial AD (FAD) subiculum. (i) Co-immunostaining of astrocytes with GFAP (pink) and NLRP1 (brown) in an AD brain. (j) Immunostaining of NLRP1 and TauΔCasp6 in 4 μm consecutive sections of an AD brain. Scale is 50 μm for i and j

The NLRP1–Casp1–Casp6 pathway increases Aβ42 in primary human neuron cultures

To assess whether the NLRP1–Casp1–Casp6 pathway is involved in AD pathogenesis, primary human neurons were serum deprived after targeted knock-down expression of NLRP1, CASP1, and CASP6 with siRNAs (Figures 3 and 4). The human Aβs 38, 40, and 42 (Aβ38, Aβ40, Aβ42) measured in a multiplex ELISA assay, revealed a statistically significant two-fold increase in the ratio of Aβ42 relative to total Aβ peptides measured in serum-deprived neurons (Figure 7a). This was consistent with our previous experiments,40, 41 and previously published data showing an increase in Aβ42 ratio in AD.42 Neurons pre-treated with NLRP1, CASP1, or CASP6 siRNAs lost that statistically significant increase of Aβ42, indicating that the NLRP1–Casp1–Casp6 pathway increased the ratio of Aβ42 peptides. NLRP1 siRNA in serum-deprived conditions did not significantly decrease Aβ42, whereas both CASP1 and CASP6 siRNAs did, suggesting that NLRP1 may not the only inflammasome activating Casp1 in serum-deprived conditions. This was consistent with increased AIM2 mRNA levels in serum-deprived neurons (Figure 2b). Furthermore, each individual human neuron preparations were not genetically identical and could respond differentially. Nevertheless, these results provide a strong mechanistic link between the NLRP1–Casp1–Casp6 pathway and AD-associated amyloid pathology.

Figure 7.

Figure 7

Open in a new tab

Nlrp1-mediated increased Aβ42 ratios in serum-deprived neurons. (a) Levels of Aβ42 relative to total Aβ peptides (38, 40, and 42) secreted from serum-deprived primary human neurons pre-treated with scrambled (Scr), NLRP1, CASP1, or CASP6 siRNAs. Data represents the mean and S.E.M. of four independent experiments. ANOVA (P<0.02) and post hoc Dunnett analysis comparing each condition with serum-treated normal neurons (*P≤0.05) or with serum-deprived scrambled siRNA-treated neurons (#P≤0.05). (b) Schematic diagram of a model linking intraneuronal Nlrp1 inflammasome activation to Caspase-1-generated IL-1β-mediated neuroinflammation and Caspase-6-mediated axonal degeneration and Aβ42 over-production

Discussion

In this study, we (1) show the complexity of inflammasome receptor gene expression in human brain cell types, (2) identify functional NLRP1 inflammasome in response to non-pathogenic insults of human CNS neurons, (3) identify a NLRP1–Casp1–Casp6 pathway in human neurons that links neuron-specific NLRP1 inflammasome activation with IL-1β-mediated neuroinflammation and axonal degeneration-associated Casp6 activation, and (4) show that NLRP1 is increased in AD neurons, co-localizes with Casp6 activity, and that the NLRP1–Casp1–Casp6 pathway is involved in serum deprivation-induced Aβ42 ratios.

Our results suggest a high degree of complexity for inflammasome-mediated responses in human brain based on the following: (1) differential gene expression of inflammasome receptors in human CNS neurons, astrocytes, and microglia primary cultures, and in normal and AD cerebellum and cortical tissues, and (2) the modulation of inflammasome receptor levels in primary cell cultures submitted to different stressors. Functional inflammasomes have been well characterized in macrophages and microglia, and recently the expression and functionality of these inflammasomes were described in rodent neurons.43 Casp1 is expressed and activated in neurons and therefore is likely to be activated via the inflammasomes. Here, we took advantage of human CNS primary neuron cultures to explore the potential impact of inflammasomes in these cell types. Human neurons expressed three (NLRP1, AIM2, and IPAF-1) of the four well-described immune cell inflammasome receptors and the ASC component of the inflammasome suggesting for the possibility of functional neuronal inflammasomes. These three inflammasome receptors were fully functional in a neuron cell-free system. The expression of specific inflammasome receptors varied in human primary cultures of astrocytes, microglia, and neurons. Nevertheless, neurons contained a relatively higher level of NLRP1 and AIM2 than astrocytes and microglia. Surprisingly, NLRP3 mRNA was not detected in naive human neuron, astrocyte, or microglial cultures and in AD or non-AD human cortical and cerebellar tissues, NLRP3 expression was not induced in serum-deprived or BzATP-treated neuron cultures, but was induced with LPS in microglia. This latter finding is consistent with the absence of NLRP3 immunohistochemical detection in healthy human CNS tissue in contrast to its presence in peripheral tissues.36 Similar to LPS, amyloid-β-peptide and mycobacteria induce NLRP3-dependent mouse microglial activation.23, 24, 44 Furthermore, spatial memory deficits induced in familial AD-associated mutant APP/PS1 mice were abrogated on a null NLRP3 background.45 Although this differential expression could be species dependent, others have observed NLRP3 in human neuronal cultures,37 suggesting that culture conditions may alter the levels of inflammasome receptors in primary human neuronal cell types. Cells respond to their extracellular environment so the conditions of cell culture could strongly influence the expression of certain genes. In the study by Ramaswamy et al.,37 the methods indicated that cells were passaged once for astrocytes, neurons, and microglia, whereas in our cultures, neurons were maintained as primary cells (no passage) and treated with fluorodeoxyuridine (FDU) to prevent astrocyte proliferation. Microglia were lost in FDU-treated cultures. Another difference is that Ramaswamy et al.,37 cultured their cells in 10% serum whereas we used 5% decomplemented serum. It has been reported that complement can activate the NLRP3 inflammasome in dendritic cells46 and a similar mechanism in primary human neurons would explain the difference between our results. Nevertheless, as each inflammasome is activated by specific DAMPs and PAMPs, these results suggest that human neurons, astrocytes, and microglia are equipped to launch a differential response to inflammasome activating agents.

In this study, we find that the human neuronal NLRP1 inflammasome is responsible for Casp1 activation in human neuron cultures submitted to stressors. Antibodies against NLRP1 in cell-free extracts, NLRP1 siRNAs in primary neuron cultures, and Nlrp1 null mice abrogate stress-mediated Casp1 activity and IL-1β production. Furthermore, NLRP1 is increased significantly in sporadic and familial AD hippocampal and cortical neurons suggesting an important role for NLRP1 in AD neurons, and the NLRP1–Casp1–Casp6 pathway increases the ratio of Aβ42 relative to total Aβ peptides. The recent association of genetic polymorphisms of the NLRP1 gene with AD further highlights the potential implication of NLRP1 in AD.47 Recently, Nlrp1 has been implicated in the behavioral deficits, cell death, Casp1 activation, and IL-1β production of mutant presenilin 1 ΔE9/APP Swedish (PS1ΔE9/APPSw) transgenic mice.48 Indeed, Nlrp1 protein increased in 5- to 9-month-old PS1ΔE9/APPSw mice and injection of Nlrp1 siRNAs in mice brains reduced apoptosis, Casp1, IL-1β production, amyloid plaques, and corrected memory deficits. Furthermore, Nlrp1 siRNAs reduced the susceptibility of primary rat cortical neurons to 5 μM Aβ42. Human primary neurons are not susceptible to Aβ-mediated toxicity, unless neurons are already vulnerable.9 We therefore propose a novel model that consolidates both our results and those of Tan (Figure 7b). We hypothesize that an age-dependent neuronal stress such as a reduction in growth factors, oxidative stress, or environmental causes in sporadic AD or the expression of mutant APP, PS1, or presenilin 2 (PS2) in familial AD, induces the activation of NLRP1. Activated NLRP1 recruits and activates Casp1, which converts pro-IL-1β into secreted IL-1β, thus activating a second phase of inflammation in astrocytes and microglia.6 Simultaneously, Casp1 activates Casp6-mediated axonal degeneration9, 11, 13 and increased production of Aβ42.41 Aβ42 becomes toxic to already vulnerable human neurons expressing FAD-associated mutant genes or submitted to age-dependent insults, or very high Aβ42 concentrations activate the Nlrp1 in rodent neurons as demonstrated by Tan.48 Note that rodents express three paralogous Nlrp1 genes, whereas humans only express one NLRP1 gene.43 That the human Nlrp1 protein N-terminus contains a pyrin protein interacting domain, whereas the mouse Nlrp1 contains a NR100 domain of unknown function could explain variability between rodent and human Nlrp1 activation.

NLRP1 is activated by Bacillus anthracis in rodents but not in humans, but the human NLRP1 has been linked to Crohn's disease and bacterial meningitis in humans (reviewed by Walsh et al.43). Functional Nlrp1 inflammasome has also been observed in rat traumatic brain and spinal cord injury and anti-NLRP1 neutralizing antibodies reduced Casp1 activation in a mouse model of stroke.27, 28, 29 However, this is the first time that functional NLRP1 is directly observed in human neurons and in AD brains.

Another important finding of this study is the identification of neuronal NLRP1 as an upstream activator of neuroinflammation and axonal degeneration. The neuronal NLRP1–Casp1–Casp6 pathway in neurons submitted to two non-pathogenic insults suggests that neuronal inflammasome activation is not necessarily activated by microbial pathogens. The NLRP1–Casp1–Casp6 pathway was confirmed in vivo by the abrogation of Casp1 and Casp6 activities in LPS-treated mice brains lacking Nlrp1 or Casp1. Furthermore, NLRP1 co-localized with TauΔCasp6 in AD neurons supporting the possibility that the activation of Casp6 occurs via NLRP1-induced Casp1 activation in AD. This NLRP1–Casp1–Casp6 pathway may explain the lack of non-steroidal anti-inflammatory drugs (NSAID) efficiency against AD because NSAID specifically target cyclooxygenase prostaglandins and do not inhibit inflammasome activity. Therefore, better therapeutic targets for AD might be NLRP1 or Casp1. Although there are three inhibitors against IL-1β on the market,49 inhibition of IL-1β would help with neuroinflammation but not with Casp6-mediated axonal degeneration. To our knowledge, there are no NLRP1 inhibitors. However, there is one Casp1 inhibitor, VX-765, in phase II clinical trials against epilepsy.50 Our results suggest that this drug might be of benefit against neuroinflammation, axonal degeneration, and cognitive impairment early in AD.

Materials and Methods

Neuronal cell cultures and cell lines

Primary human neurons were cultured as described previously51 and in accordance with a protocol approved by the McGill Institutional Review Board. Brain cortical tissues were obtained from Novagenix (Belmont, CA, USA) or the Birth Defects Research Laboratory (BRDL, University of Washington, Seattle). Briefly, 12- to 16-week-old normal fetal brains were dissociated with trypsin, treated with deoxyribonuclease I, and filtered through 130 and 70 micron nylon mesh. The dissociated cells were centrifuged for 10 min at 1200 r.p.m. and resuspended in minimal essential medium (MEM) with Earle's salts, supplemented with 5% decomplemented BCS, 0.1% Glucose (Sigma, St Louis, MO, USA), 1 × Penicillin-Streptomycin, 1 mM sodium pyruvate, and 2 mM l-glutamine (Invitrogen, Carlsbad, CA, USA). Cells were seeded at a density of 3 × 106 cells/ml on poly-lysine-coated tissue culture flasks or plates. The medium was changed every 2 days. Astrocyte proliferation was limited with the treatment of the antimitotic agent, FDU (Sigma, St Louis, MO, USA; 1 mM) for the first 4 days. Neuronal cultures were grown for 7-10 days before conducting experiments. The THP-1 monocyte cell line, a gift from Dr. John Hiscott (Lady Davis Institute) was used as a positive control for inflammasome components. THP-1 were grown in suspension in RPMI medium supplemented with 10% FBS (Invitrogen). Neuronal or astrocyte cultures were exposed to serum withdrawal or 2'(3')-O-(4-Benzoylbenzoyl)adenosine-5'-triphosphate tri(triethylammonium) salt (BzATP; Sigma). Casp1 inhibitor Z-YVAD-fmk was purchased from BioMol (Philadelphia, PA, USA). Experiments were conducted on a minimum of three independent cellular preparations.

Astrocyte and microglia cultures

Purified microglia and astrocyte cultures were prepared from fetal tissue as previously described.51 However, unlike neuronal culture preparation, astrocytic and microglial media was changed after 1 day of seeding and cultures were not treated with 1 mM FDU. After 21 days, the flasks were shaken on an orbital shaker for 15 min to dislodge the microglia from the underlying bed of astrocytes. Harvested microglia were centrifuged for 10 min at 1200 r.p.m. and resuspended in supplemented MEM (see above). The remaining bed of astrocytes were removed with 0.25% trypsin and plated at a density of 2 × 105 cells/ml. Both microglia and astrocyte cultures were used in experiments after 48 h.

Caspase activity assays

Neuronal or astrocyte cultures were either serum-deprived or treated with 500 μM BzATP for up to 24 h. At various time intervals, neuronal proteins were extracted in cell lysis buffer (50 mM HEPES, pH 7.4, 0.1% CHAPS, 0.1 mM EDTA), containing a cocktail of protease inhibitors (38 μg/ml AEBSF (4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride), 0.5 μg/ml Leupeptin, 0.1 μg/ml Tosyl-L-lysyl-chloromethane hydrochloride (TLCK), 0.1 μg/ml Pepstatin (Sigma)). Samples were stored at −20 °C until ready to assay. Caspase-1, -6 and -3 activities were measured as previously described.19 Briefly, before assessing caspase activity, protein concentration from each sample was quantified using the Bradford colorimetric protein assay and BSA standards. Neuronal protein extract (10–20 μg) was mixed with a Caspase reaction mixture, containing 20 mM PIPES, pH 7.4, 30 mM NaCl, 0.1% CHAPS, 1 mM EDTA, 10% sucrose, and 10 mM DTT, and either 10 μM Ac-YVAD-AFC for Casp1 or Ac-VEID-AFC for Casp6 or Ac-DEVD-AFC for Casp3 activities. Activity was measured using a plate reader (Bio-Tek Synergy H4, Winooski, VT, USA) with excitation at 380 nm and emission 505 nm. Measurements were read every 2 min for 1.5 h and released moles of amino fluorocoumarin (AFC) were calculated from a standard curve of AFC. Specific activity for each sample was determined and standardized to protein concentration.

Conventional RT-PCR

Total RNA was isolated with TRIzol reagent (Invitrogen), according to the manufacturer's instructions and cDNA synthesis was preformed as previously described.52 PCR products from synthesized cDNA were amplified with specific primers; NLRP1 Forward 5′-TCCCCCTTGGGAGTCCTCCTGAAAATG-3′ and Reverse 5′-CGAGAACAGCTGGTCTTCTCCAGGGCTTC-3′ NLRP3 Forward 5′-AAAAGACTCATCCGTGTGCC-3′ and Reverse 5′-TTCCTGGCATATCACAGTGG-3′ AIM2, Forward 5′-GCAGTGATGAAGACCATTCGTA-3′ and Reverse 5′-GCTGAGTTTGAAGCGTGTTGAT-3′ IPAF-1, Forward 5′-CTCTCATGGTGGAA GCCAGTCC-3′ and Reverse 5′-GACAGAGACTTGACTATGTAATCC-3′ ASC, Forward 5′-GCTGTCCATGGACGCCTTGG-3′ and Reverse 5′-CATCCGTCAGGACCTTCCCGT-3′ CASP1, Forward 5′-GAAGGCATT TGTGGGAAGAA-3′ and Reverse 5′-CATCTGGCTGCTCAAATGAA-3′ CASP6, Forward 5′-CGATGTGCCAGTCATTCCTT-3′ and Reverse 5′-CTCTAAGGAG GAGCCATATTTTC-3′ GFAP, Forward 5′-GTGGGCAGGTGGGAGCTTGATTCT-3′ and Reverse 5′-CTGGGGCGGCCTGGTATGACA-3′ MAP2, Forward 5′-GCAGTTCTCAAAGGCTAGAC-3′ and Reverse 5′-TTGATCGTGGAACTCCATCT-3′ CD68, Forward 5′-GGGACCCTCAACTGCCACTCA-3′ and Reverse 5′-AGAGAAGCAGGTGGGGATGGG-3′ P2X7R, Forward 5′-GAAGGCATTTGT GGGAAGAA-3′ and Reverse 5′-CATCTGGCTGCTCAAATGAA-3′ GAPDH Forward 5′-ACCACAGTCCATGCC-3′ and Reverse 5′-TCCACCACCCTGTTG-3′. Optimal annealing temperature was determined for each primer set using a temperature gradient. 2 μl of cDNA was used for each PCR reaction using Taq DNA polymerase (New England Biolabs, Whitby, ON, Canada), according to the manufacturer's protocol. The products were visualized on ethidium bromide stained 2% agarose gels.

qRT-PCR

Real-time quantitative reverse transcription (qRT)-PCR was used to measure gene transcript levels as previously described,53 using commercially available pre-validated primers (Origene, Rockville, MD, USA) for NLRP1, IPAF-1, and AIM2. THP-1 monocyte mRNA was used as internal control for experiments. Human AD and control cortex and cerebellum tissues were provided by Dr. Bradley Hyman. Briefly, TRIzol reagent (Invitrogen) was used to harvest and isolate RNA according to the manufacturer's protocol. Total RNA (1 μg) was reverse transcribed using 40 units of AMV-reverse transcriptase, with 1 mM dNTPs (Promega, Madison, WI, USA) and 5 μM oligo-dT (Invitrogen). qPCR was performed on cDNA using SYBR Green Taq Master Mix (Quanta Biosciences, Gaithersburg, MD, USA). mRNA amounts were calculated for each gene of interest with the housekeeping gene GAPDH or 18 S using the ΔΔCT method.54 The efficiency of each primer was quantified to insure no differences in amplification were due to the primer efficiencies. mRNA levels were expressed as relative fold changes.

IL-1β ELISA

Medium was collected from untreated neurons and neurons exposed to serum-deprivation or BzATP for 24 h, with or without 5 μM Z-YVAD-fmk and DMSO. IL-1β levels in media were quantified using an ELISA kit, according to the manufacturer's instructions (BD Biosciences, Mississauga, ON, Canada). For mice samples, IL-1β levels were measured from frontal cortex tissue extracted in cell lysis buffer, using a mouse-specific ELISA kit (BD Biosciences). IL-1β levels were standardized to total protein concentration.

Casp1 and NLRP1 siRNA experiments

Neurons were transfected with 1 μM scrambled, NLRP1 or CASP1 Accell siRNA (ThermoScientific/Fisher, Ottawa, ON, Canada) for 72 h. Neuronal serum-deprivation or BzATP treatments were performed for 30 min or 60 min and neurons were either harvested in cell lysis buffer for protein analysis or in TRIzol (Invitrogen) to isolate RNA. Media was harvested for ELISA IL-1β experiments.

YO-PRO1 dye uptake

Assessment of functional P2X7R was measured by YO-PRO1 dye (Invitrogen) uptake in neurons. Neuronal cultures were seeded in a 96-well clear bottom, black-sided Costar plates (Corning, NY, USA) at a density of 50 000 cells per well. Following 7 days of culturing, neurons were treated with 500 μM BzATP, with or without 10 μM BBG (Bio-Rad, Mississauga, ON, Canada) and 1 mM YO-PRO1 dye. At specific times (15 min, 30 min, 45 min, 1 h, 3 h, and 6 h), YO-PRO1 dye fluorescence was measured using a Bio-Tek spectrofluorometer with excitation at 491 nm and emission 591 nm. Background fluorescence levels from a well-containing medium only, were subtracted from all values and expressed as RFU per 50 000 cells.

Cell-free inflammasome system

Neuronal inflammasome function was assessed by obtaining the S-100 cytosolic subcellular fractions as previously developed and described for THP-1 monocytes.38 Neuronal cultures were either untreated or exposed to serum-free conditions for 15 min. Cells were washed with ice-cold PBS, lysed in ice-cold hypotonic buffer (20 mM HEPES-KOH (pH 7.5), 10 mM KCl, 1.5 mM MgCl2, 1 mM Na-EDTA, 1 mM Na-EGTA, 38 μg/ml AEBSF, 0.5 μg/ml Leupeptin, 0.1 μg/ml Pepstatin, 0.1 μg/ml TLCK), and incubated on ice for 15 min. The cellular membrane integrity was disrupted using a Dounce homogenizer (Sigma). Cell lysates were centrifuged at 1000 × g to remove membrane fractions. The supernatant was harvested and centrifuged at 100 000 × g and the resulting S-100 supernatants were stored at −80 oC. S-100 fractions were incubated with or without 1-2 μg/ml of commercially available antibodies against each inflammasome; (NLRP1 (Cell Signaling, Danvers, MA, USA), AIM2 (Novus Biologicals, Oakville, ON, Canada), and IPAF-1 (Thermo Fisher Scientific, Rockford, IL, USA)), or with specific concentrations of inflammasome activators Poly(deoxyadenylic-thymidylic) acid sodium salt (PolydAT, Sigma), MDP (Invitrogen) and recombinant flagellin (Invivogen, San Diego, CA, USA).

In vitro α-tubulin cleavage

Full length bovine α-tubulin (Cytoskeleton Inc., Denver, CO, USA) at 600 ng/μl was incubated with 0.02 to 0.06 U/μL recombinant active Caspase-1 (BioVision, Milpitas, CA, USA) in a caspase reaction mix (20 mM DTT, 20 mM PIPES, pH 7.4, 30 mM NaCl, 0.1% CHAPS, 1 mM EDTA, and 10% sucrose) overnight at 37 oC. Recombinant active Casp6 (200 ng/μl) was used as a positive control. Samples were boiled in Laemmli buffer and analyzed by western blot.

In vivo LPS mouse injections

All animal experiments were conducted in accordance with protocols approved by the McGill Animal Care Facility or Institutional Animal Care and Use Committee guidelines of the University of North Carolina at Chapel Hill. LPS from Escherichia coli 0111:B4 were purchased from Sigma. 18–22 weeks old C57BL/6 J (The Jackson Laboratory, Bar Harbor, ME, USA), Nlrp1−/− (generated by Rebecca Dye in Dr. Beverly Koller's Lab, UNC-Chapel Hill, NC, USA), and Casp1−/− (The Jackson Laboratory) male mice were IP injected with a single dose of 5 mg/kg LPS for 6 h. Mice were euthanized using CO2 and brains were extracted. Frontal cortex was dissected and was homogenized in cell lysis buffer (50 mM HEPES, pH 7.4, 0.1% CHAPS, 0.1 mM EDTA), containing a cocktail of protease inhibitors (38 μg/ml AEBSF (4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride), 0.5 μg/ml Leupeptin, 0.1 μg/ml TLCK, 0.1 μg/ml Pepstatin; Sigma) or TRIzol (Invitrogen) using a ground-glass homogenizer. Tissue was assessed for mRNA and protein analyses.

Immunohistochemistry and quantification

Non-AD and AD tissue was obtained with informed consent from the patients or their nearest relative and according to institutional ethical guidelines. Non-AD (1401, 4593, and 4641) and AD (1048, 1459, and 2992) brains used for immunohistochemistry were a kind gift from Dr. Catherine Bergeron (University of Toronto, Canada). Dr. Bernardino Ghetti provided familial AD tissues. Tissue sections were deparaffinized, rehydrated, and treated with citrate antigen retrieval buffer (0,01M Tris-Na Citrate, pH 6) for 20 min at 97 °C in the Pascal Dako Cytomation apparatus. The immunostaining was done using the Dako Autostainer Plus automated slide processor and the EnVision Flex system (Dako, Burlington, ON, Canada) or EnVision G/2 Doublestain System (Dako) for co-staining. The antibodies used were against human NLRP1 (1/300; LifeSpan BioSciences, Inc, Seattle, WA, USA), GFAP (1/8000; Dako) and laboratory-made TauΔCasp6 (10635: 1/12500). Slides were counterstained with haematoxylin, dehydrated, mounted in Permount mounting medium (Fisher Scientific, Ottawa, ON, Canada) and digitally scanned with the MIRAX SCAN (Zeiss, Don Mills, ON, Canada). Using the scanned tissue sections from the Mirax digital scanner (Don Mills, ON, Canada) five images were taken at magnification × 20 from three AD and three non-AD cases.

Familial AD cases (2002-086, 2008-073, 2006-093, 2007-093) used for quantification studies were a kind gift from Dr. Bernardino Ghetti (Indiana Alzheimer Disease Center, Indiana University Medical Center, Indianapolis, IN, USA). For NLRP1 staining quantification, ImageJ software (NIH, Bethesda, MD, USA) was used to measure the surface covered by staining in neurons. The results were expressed as NLRP1 μm2/mm2 subiculum. Statistics were performed using one-way ANOVA with a Tukey-Kramer post hoc test.

Western blotting

25–50 μg of neuronal protein or mouse cortex lysate were prepared in Laemmli buffer and electrophoresed on a 15% SDS-PAGE gel. Laboratory-made anti-Casp6p20 10630 neoepitope antibody (1 : 10 000) was used to detect the cleaved p20 subunit of active Casp6 and anti-tubulin cleaved by Casp6 (TubΔCasp6) GN60622 neoepitope antibody (1 : 10 000) was used to detect Casp6-cleaved tubulin fragments. β-actin monoclonal antibody (Sigma, 1 : 5000) and full-length α-tubulin antibody (Cell Signaling, Danvers, MA, USA, 1 : 1000) were used as loading controls. For the inflammasome receptors, neuronal protein were extracted in cell lysis buffer (20 mM HEPES, pH 7.5, 50 mM KCl, 150 mM NaCl, 1.5 mM MgCl2, 1 mM EGTA, 0.5%, NP-40, 1 mM DTT, 0.159 mM AEBSF, 1.458 mM pepstatin, 1.17 mM leupeptin, 0.27 mM TLCK), microcentrifuged 15 min at high speed, and 50 μg supernatant protein loaded on 10-15% SDS-PAGE. Western blots were probed with anti-NLRP1 (Enzo Life Sciences, Farmingdale, NY, USA; diluted 1 : 1000), anti-AIM2 (1 : 1000 dilution from Cell Signaling, Beverly, MA, USA), anti-IPAF-1 (anti-CLAN 1/1000 from Pierce Thermo Scientific, Rockford, IL, USA). Secondary antibodies conjugated with HRP were from Dako (anti-rabbit) and GE HealthCare (Baie-D'Urfe, QC, Canada) (anti-mouse). ImageJ software was used to quantify band intensities in triplicate gels.

Analysis of secreted Aβ peptides

Human primary neurons were treated with 1 μM Accell siRNAs against NLRP1, CASP1, and CASP6 (controlled with scrambled siRNAs) for 72 h. Neurons were then serum-deprived for 24 h. Media was collected and frozen until analysis with the Mesoscale Discovery (MSD; Rockville, MD, USA) human Aβ panel 1 (6E10) kit as directed by the manufacturer.

ASC-GFP experiments

The pCI-ASC-HA (human ASC) construct, deposited by Kate Fitzgerald (UMass., MA)55 was obtained from Addgene (Cambridge, MA, USA). The pCI-ASC-HA was used as a template to amplify the human ASC with ASC_BglII_For primer 5′-CCGCTAAGATCTGCCATGGGGCGCGCGCGCGACGCCATCC-3′ and ASC_AgeI_Rev primer 5′-ACGCGAACCGGTGACCGCTCCAGGTCCTCCACC-3′. The amplicon was cloned into the pEGFP-N1 construct to produce the pASC-EGFP expression construct. Neurons were treated with NLRP1, CASP1, or CASP6 siRNAs as described above and transfected with the ASC-GFP. After 24 h, neurons were serum deprived and pictures of GFP-positive neurons taken under the fluorescence microscope. The area of fluorescence was quantitated. The experiment was repeated in two independent neuron preparations with 30–60 neurons per condition.

Statistical analyses

All data are presented as the mean of each treatment group±S.E.M. The statistical significance of differences between experimental groups was analyzed using InStat software (GraphPad Software, La Jolla, CA, USA) as indicated in the figure legends.

Acknowledgments

We thank Marc-André Déry for generating the ASC-GFP construct. The Canadian Foundation of Innovation, Canadian Institutes of Health Research (CIHR) MOP-243413-BCA-CGAG-45097, and the JGH Foundation (ALB), and NIH Grant P30 AG010133 (BG) supported this work. Dr. Vikas Kaushal was a recipient of McGill's CIHR Neuroinflammation Training Program award (2010-2012); Prateep Pakavathkumar is the recipient of a Fonds de recherche Québec-Santé scholarship (2011–2013) and the FRSQ-Alzheimer Society of Canada doctoral award (2014–2016).

Glossary

Aβ

amyloid beta peptide

AD

Alzheimer disease

AIM2

absent in melanoma 2

ASC

apoptosis speck-like protein containing a caspase recruitment domain

BzATP

benzylated ATP

BBG

Brilliant Blue G

CA1

Cornus ammonis region 1

Casp1

Caspase-1

Casp6

Caspase-6

CD68

cluster of differentiation 68

DAMP

danger-associated molecular patterns

FDU

fluorodeoxyuridine

GFAP

glial fibrillary acidic protein

IL-1β

Interleukin-1-beta

IPAF-1

ICE protease activating factor

MAP2

microtubule-associated protein 2

MDP

muramyl dipeptide

NLRP1

Nod-like receptor protein 1

NLRP3

Nod-like receptor protein 3

NSAID

non-steroidal anti-inflammatory drugs

PAMP

pathogen-associated molecular patterns

Footnotes

Supplementary Information accompanies this paper on Cell Death and Differentiation website (http://www.nature.com/cdd)

Edited by L Greene

Supplementary Material

Supplementary Information
Click here for additional data file. (1MB, pdf)

References

  1. Reitz C, Brayne C, Mayeux R. Epidemiology of Alzheimer disease. Nat Rev Neurol. 2011;7:137–152. doi: 10.1038/nrneurol.2011.2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kamboh MI, Demirci FY, Wang X, Minster RL, Carrasquillo MM, Pankratz VS, et al. Genome-wide association study of Alzheimer's disease. Transl Psychiatry. 2012;2:e117. doi: 10.1038/tp.2012.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hollingworth P, Harold D, Sims R, Gerrish A, Lambert JC, Carrasquillo MM, et al. Common variants at ABCA7, MS4A6A/MS4A4E, EPHA1, CD33 and CD2AP are associated with Alzheimer's disease. Nat Genet. 2011;43:429–435. doi: 10.1038/ng.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Zhang B, Gaiteri C, Bodea LG, Wang Z, McElwee J, Podtelezhnikov AA, et al. Integrated systems approach identifies genetic nodes and networks in late-onset Alzheimer's disease. Cell. 2013;153:707–720. doi: 10.1016/j.cell.2013.03.030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Griffin WS, Stanley LC, Ling C, White L, MacLeod V, Perrot LJ, et al. Brain interleukin 1 and S-100 immunoreactivity are elevated in Down syndrome and Alzheimer disease. Proc Natl Acad Sci USA. 1989;86:7611–7615. doi: 10.1073/pnas.86.19.7611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Shaftel SS, Griffin WS, O'Banion MK. The role of interleukin-1 in neuroinflammation and Alzheimer disease: an evolving perspective. J Neuroinflammation. 2008;5:7. doi: 10.1186/1742-2094-5-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jaturapatporn D, Isaac MG, McCleery J, Tabet N. Aspirin, steroidal and non-steroidal anti-inflammatory drugs for the treatment of Alzheimer's disease. Cochrane Database Syst Rev. 2012;2:CD006378. doi: 10.1002/14651858.CD006378.pub2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Terry R, Masliah E, Salmon D, Butters N, DeTeresa R, Hill R, et al. Physical basis of cognitive alterations in Alzheimer's disease: synapse loss is the major correlate of cognitive impairment. Ann Neurol. 1991;30:572–580. doi: 10.1002/ana.410300410. [DOI] [PubMed] [Google Scholar]
  9. Sivananthan SN, Lee AW, Goodyer CG, LeBlanc AC. Familial amyloid precursor protein mutants cause caspase-6-dependent but amyloid beta-peptide-independent neuronal degeneration in primary human neuron cultures. Cell Death Dis. 2010;1:e100. doi: 10.1038/cddis.2010.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Simon DJ, Weimer RM, McLaughlin T, Kallop D, Stanger K, Yang J, et al. A caspase cascade regulating developmental axon degeneration. J Neurosci. 2012;32:17540–17553. doi: 10.1523/JNEUROSCI.3012-12.2012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nikolaev A, McLaughlin T, O'Leary DD, Tessier-Lavigne M. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature. 2009;457:981–989. doi: 10.1038/nature07767. [DOI] [PMC free article] [PubMed] [Google Scholar] [Retracted]
  12. Cusack CL, Swahari V, Hampton Henley W, Michael Ramsey J, Deshmukh M. Distinct pathways mediate axon degeneration during apoptosis and axon-specific pruning. Nat Commun. 2013;4:1876. doi: 10.1038/ncomms2910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Uribe V, Wong BK, Graham RK, Cusack CL, Skotte NH, Pouladi MA, et al. Rescue from excitotoxicity and axonal degeneration accompanied by age-dependent behavioral and neuroanatomical alterations in caspase-6-deficient mice. Hum Mol Genet. 2012;21:1954–1967. doi: 10.1093/hmg/dds005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Guo H, Albrecht S, Bourdeau M, Petzke T, Bergeron C, LeBlanc AC. Active caspase-6 and caspase-6-cleaved tau in neuropil threads, neuritic plaques, and neurofibrillary tangles of Alzheimer's disease. Am J Pathol. 2004;165:523–531. doi: 10.1016/S0002-9440(10)63317-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Albrecht S, Bogdanovic N, Ghetti B, Winblad B, LeBlanc AC. Caspase-6 activation in familial alzheimer disease brains carrying amyloid precursor protein or presenilin i or presenilin II mutations. J Neuropathol Exp Neurol. 2009;68:1282–1293. doi: 10.1097/NEN.0b013e3181c1da10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Braak H, Alafuzoff I, Arzberger T, Kretzschmar H, Del Tredici K. Staging of Alzheimer disease-associated neurofibrillary pathology using paraffin sections and immunocytochemistry. Acta Neuropathol (Berl) 2006;112:389–404. doi: 10.1007/s00401-006-0127-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ramcharitar J, Afonso VM, Albrecht S, Bennett DA, LeBlanc AC. Caspase-6 activity predicts lower episodic memory ability in aged individuals. Neurobiol Aging. 2013;34:1815–1824. doi: 10.1016/j.neurobiolaging.2013.01.007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LeBlanc AC, Ramcharitar J, Afonso V, Hamel E, Bennett DA, Pakavathkumar P, et al. Caspase-6 activity in the CA1 region of the hippocampus induces age-dependent memory impairment. Cell Death Differ. 2014;21:696–706. doi: 10.1038/cdd.2013.194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Guo H, Petrin D, Zhang Y, Bergeron C, Goodyer CG, LeBlanc AC. Caspase-1 activation of caspase-6 in human apoptotic neurons. Cell Death Differ. 2006;13:285–292. doi: 10.1038/sj.cdd.4401753. [DOI] [PubMed] [Google Scholar]
  20. Schroder K, Tschopp J. The inflammasomes. Cell. 2010;140:821–832. doi: 10.1016/j.cell.2010.01.040. [DOI] [PubMed] [Google Scholar]
  21. Martinon F, Tschopp J. Inflammatory caspases and inflammasomes: master switches of inflammation. Cell Death Differ. 2007;14:10–22. doi: 10.1038/sj.cdd.4402038. [DOI] [PubMed] [Google Scholar]
  22. Stutz A, Golenbock DT, Latz E. Inflammasomes: too big to miss. J Clin Invest. 2009;119:3502–3511. doi: 10.1172/JCI40599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shi F, Yang L, Kouadir M, Yang Y, Wang J, Zhou X, et al. The NALP3 inflammasome is involved in neurotoxic prion peptide-induced microglial activation. J Neuroinflammation. 2012;9:73. doi: 10.1186/1742-2094-9-73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lee HM, Kang J, Lee SJ, Jo EK. Microglial activation of the NLRP3 inflammasome by the priming signals derived from macrophages infected with mycobacteria. Glia. 2013;61:441–452. doi: 10.1002/glia.22448. [DOI] [PubMed] [Google Scholar]
  25. Frederick LoC, Ning X, Gonzales C, Ozenberger BA. Induced expression of death domain genes NALP1 and NALP5 following neuronal injury. Biochem Biophys Res Commun. 2008;366:664–669. doi: 10.1016/j.bbrc.2007.11.174. [DOI] [PubMed] [Google Scholar]
  26. Liu F, Lo CF, Ning X, Kajkowski EM, Jin M, Chiriac C, et al. Expression of NALP1 in cerebellar granule neurons stimulates apoptosis. Cell Signal. 2004;16:1013–1021. doi: 10.1016/j.cellsig.2004.02.006. [DOI] [PubMed] [Google Scholar]
  27. Abulafia DP, de Rivero Vaccari JP, Lozano JD, Lotocki G, Keane RW, Dietrich WD. Inhibition of the inflammasome complex reduces the inflammatory response after thromboembolic stroke in mice. J Cereb Blood Flow Metab. 2009;29:534–544. doi: 10.1038/jcbfm.2008.143. [DOI] [PubMed] [Google Scholar]
  28. de Rivero Vaccari JP, Lotocki G, Alonso OF, Bramlett HM, Dietrich WD, Keane RW. Therapeutic neutralization of the NLRP1 inflammasome reduces the innate immune response and improves histopathology after traumatic brain injury. J Cereb Blood Flow Metab. 2009;29:1251–1261. doi: 10.1038/jcbfm.2009.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. de Rivero Vaccari JP, Lotocki G, Marcillo AE, Dietrich WD, Keane RW. A molecular platform in neurons regulates inflammation after spinal cord injury. J Neurosci. 2008;28:3404–3414. doi: 10.1523/JNEUROSCI.0157-08.2008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Fann DY, Lee SY, Manzanero S, Tang SC, Gelderblom M, Chunduri P, et al. Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke. Cell Death Dis. 2013;4:e790. doi: 10.1038/cddis.2013.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Silverman WR, de Rivero Vaccari JP, Locovei S, Qiu F, Carlsson SK, Scemes E, et al. The pannexin 1 channel activates the inflammasome in neurons and astrocytes. J Biol Chem. 2009;284:18143–18151. doi: 10.1074/jbc.M109.004804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Li Q, Tian Y, Wang ZF, Liu SB, Mi WL, Ma HJ, et al. Involvement of the spinal NALP1 inflammasome in neuropathic pain and aspirin-triggered-15-epi-lipoxin A4 induced analgesia. Neuroscience. 2013;254:230–240. doi: 10.1016/j.neuroscience.2013.09.028. [DOI] [PubMed] [Google Scholar]
  33. Mawhinney LJ, de Rivero Vaccari JP, Dale GA, Keane RW, Bramlett HM. Heightened inflammasome activation is linked to age-related cognitive impairment in Fischer 344 rats. BMC Neurosci. 2011;12:123. doi: 10.1186/1471-2202-12-123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Zou J, Crews FT. Inflammasome-IL-1beta signaling mediates ethanol inhibition of hippocampal neurogenesis. Front Neurosci. 2012;6:77. doi: 10.3389/fnins.2012.00077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Adamczak SE, de Rivero Vaccari JP, Dale G, Brand Iii FJ, Nonner D, Bullock M, et al. Pyroptotic neuronal cell death mediated by the AIM2 inflammasome. J Cereb Blood Flow Metab. 2014;34:621–629. doi: 10.1038/jcbfm.2013.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Kummer JA, Broekhuizen R, Everett H, Agostini L, Kuijk L, Martinon F, et al. Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response. J Histochem Cytochem. 2007;55:443–452. doi: 10.1369/jhc.6A7101.2006. [DOI] [PubMed] [Google Scholar]
  37. Ramaswamy V, Walsh JG, Sinclair DB, Johnson E, Tang-Wai R, Wheatley BM, et al. Inflammasome induction in Rasmussen's encephalitis: cortical and associated white matter pathogenesis. J Neuroinflammation. 2013;10:152. doi: 10.1186/1742-2094-10-152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Martinon F, Burns K, Tschopp J. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell. 2002;10:417–426. doi: 10.1016/s1097-2765(02)00599-3. [DOI] [PubMed] [Google Scholar]
  39. Qin L, Wu X, Block ML, Liu Y, Breese GR, Hong JS, et al. Systemic LPS causes chronic neuroinflammation and progressive neurodegeneration. Glia. 2007;55:453–462. doi: 10.1002/glia.20467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. LeBlanc A. Increased production of 4 kDa amyloid beta peptide in serum deprived human primary neuron cultures: possible involvement of apoptosis. J Neurosci. 1995;15:7837–7846. doi: 10.1523/JNEUROSCI.15-12-07837.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. LeBlanc A, Liu H, Goodyer C, Bergeron C, Hammond J. Caspase-6 role in apoptosis of human neurons, amyloidogenesis, and Alzheimer's disease. J Biol Chem. 1999;274:23426–23436. doi: 10.1074/jbc.274.33.23426. [DOI] [PubMed] [Google Scholar]
  42. Moore BD, Chakrabarty P, Levites Y, Kukar TL, Baine AM, Moroni T, et al. Overlapping profiles of Abeta peptides in the Alzheimer's disease and pathological aging brains. Alzheimers Res Ther. 2012;4:18. doi: 10.1186/alzrt121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Walsh JG, Muruve DA, Power C. Inflammasomes in the CNS. Nat Rev Neurosci. 2014;15:84–97. doi: 10.1038/nrn3638. [DOI] [PubMed] [Google Scholar]
  44. Halle A, Hornung V, Petzold GC, Stewart CR, Monks BG, Reinheckel T, et al. The NALP3 inflammasome is involved in the innate immune response to amyloid-beta. Nat Immunol. 2008;9:857–865. doi: 10.1038/ni.1636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Heneka MT, Kummer MP, Stutz A, Delekate A, Schwartz S, Vieira-Saecker A, et al. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice. Nature. 2013;493:674–678. doi: 10.1038/nature11729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Laudisi F, Spreafico R, Evrard M, Hughes TR, Mandriani B, Kandasamy M, et al. Cutting edge: the NLRP3 inflammasome links complement-mediated inflammation and IL-1beta release. J Immunol. 2013;191:1006–1010. doi: 10.4049/jimmunol.1300489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Pontillo A, Catamo E, Arosio B, Mari D, Crovella S. NALP1/NLRP1 genetic variants are associated with Alzheimer disease. Alzheimer Dis Assoc Disord. 2012;26:277–281. doi: 10.1097/WAD.0b013e318231a8ac. [DOI] [PubMed] [Google Scholar]
  48. Tan MS, Tan L, Jiang T, Zhu XC, Wang HF, Jia CD, et al. Amyloid-beta induces NLRP1-dependent neuronal pyroptosis in models of Alzheimer's disease. Cell Death Dis. 2014;5:e1382. doi: 10.1038/cddis.2014.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Kone-Paut I, Piram M. Targeting interleukin-1beta in CAPS (cryopyrin-associated periodic) syndromes: what did we learn. Autoimmun Rev. 2012;12:77–80. doi: 10.1016/j.autrev.2012.07.026. [DOI] [PubMed] [Google Scholar]
  50. Bialer M, Johannessen SI, Levy RH, Perucca E, Tomson T, White HS. Progress report on new antiepileptic drugs: a summary of the Eleventh Eilat Conference (EILAT XI) Epilepsy Res. 2013;103:2–30. doi: 10.1016/j.eplepsyres.2012.10.001. [DOI] [PubMed] [Google Scholar]
  51. LeBlanc AC, Xue R, Gambetti P. Amyloid precursor protein metabolism in primary cell cultures of neurons, astrocytes, and microglia. J Neurochem. 1996;66:2300–2310. doi: 10.1046/j.1471-4159.1996.66062300.x. [DOI] [PubMed] [Google Scholar]
  52. Lee AW, Champagne N, Wang X, Su XD, Goodyer C, Leblanc AC. Alternatively spliced caspase-6B isoform inhibits the activation of caspase-6A. J Biol Chem. 2010;285:31974–31984. doi: 10.1074/jbc.M110.152744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  53. Misiewicz M, Dery MA, Foveau B, Jodoin J, Ruths D, LeBlanc AC. Identification of a novel endoplasmic reticulum stress response element regulated by XBP1. J Biol Chem. 2013;288:20378–20391. doi: 10.1074/jbc.M113.457242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25:402–408. doi: 10.1006/meth.2001.1262. [DOI] [PubMed] [Google Scholar]
  55. Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, Caffrey DR, et al. AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC. Nature. 2009;458:514–518. doi: 10.1038/nature07725. [DOI] [PMC free article] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Information
Click here for additional data file. (1MB, pdf)

Articles from Cell Death and Differentiation are provided here courtesy of Nature Publishing Group

RESOURCES