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. 1993 Jan 1;90(1):292–296. doi: 10.1073/pnas.90.1.292

The mouse intracisternal A particle-promoted placental gene retrotransposition is mouse-strain-specific.

A Chang-Yeh 1, D E Mold 1, M H Brilliant 1, R C Huang 1
PMCID: PMC45646  PMID: 7678343

Abstract

Insertion of a single long terminal repeat (LTR) of an intracisternal A particle (IAP) gene into a placenta-expressed cellular gene (mouse IAP-promoted placental gene, MIPP) has recently been found for the mouse strains Swiss and CF-1. To test whether such a retrotransposition event has also taken place in other strains of mouse, genomic DNA and total RNAs were analyzed from the outbred CF-1 strain and five inbred strains of mouse, AKR/J, C57BL/6J, DBA/2J, C3H/HeJ, and BALB/c. Specific DNA fragments flanking the LTR region were isolated from MIPP cDNA and genomic clones and used as hybridization probes. Three transcripts [two minor, 4.4 kilobases (kb) and 2.2 kb; one major, 1.2 kb] were detected. The 4.4-kb and 2.2-kb species were found in all strains of mouse studied. The 1.2-kb transcript (promoted by IAP LTR) is present only in placentas of C3H/HeJ and BALB/c mice. Both of these strains contain an IAP LTR in the MIPP gene. In contrast, there is no IAP LTR in this cellular gene in strains DBA/2J, C57BL/6J, and AKR/J. Thus, the IAP MIPP retrotransposition is strain-specific. The parents of the outbred CF-1 mice used for the present studies were both heterozygous at the MIPP locus. Restriction fragment length polymorphism studies using inbred strains and recombinant inbred mice have further established the linkage between the MIPP gene (D4Jhu8) and several loci on distal mouse chromosome 4. The symbol Ipp is being used for this gene in all major mouse data bases and in the comparative section of the human genome data base.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atchley W. R., Fitch W. M. Gene trees and the origins of inbred strains of mice. Science. 1991 Oct 25;254(5031):554–558. doi: 10.1126/science.1948030. [DOI] [PubMed] [Google Scholar]
  2. Blank R., Eppig J., Fiedorek F. T., Jr, Frankel W. N., Friedman J. M., Huppi K., Jackson I., Mock B. Mouse chromosome 4. Mamm Genome. 1991;1(Spec No):S51–S78. doi: 10.1007/BF00656486. [DOI] [PubMed] [Google Scholar]
  3. Burt D. W., Reith A. D., Brammar W. J. A retroviral provirus closely associated with the Ren-2 gene of DBA/2 mice. Nucleic Acids Res. 1984 Nov 26;12(22):8579–8593. doi: 10.1093/nar/12.22.8579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Canaani E., Dreazen O., Klar A., Rechavi G., Ram D., Cohen J. B., Givol D. Activation of the c-mos oncogene in a mouse plasmacytoma by insertion of an endogenous intracisternal A-particle genome. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7118–7122. doi: 10.1073/pnas.80.23.7118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chang-Yeh A., Mold D. E., Huang R. C. Identification of a novel murine IAP-promoted placenta-expressed gene. Nucleic Acids Res. 1991 Jul 11;19(13):3667–3672. doi: 10.1093/nar/19.13.3667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chiu I. M., Huang R. C., Aaronson S. A. Genetic relatedness between intracisternal A particles and other major oncovirus genera. Virus Res. 1985 Jul;3(1):1–11. doi: 10.1016/0168-1702(85)90036-x. [DOI] [PubMed] [Google Scholar]
  7. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  8. Clouston W. M. The angiotensinogen gene of Swiss mice is closely linked to a retrovirus-like element. DNA Cell Biol. 1990 Nov;9(9):623–630. doi: 10.1089/dna.1990.9.623. [DOI] [PubMed] [Google Scholar]
  9. Cole M. D., Ono M., Huang R. C. Terminally redundant sequences in cellular intracisternal A-particle genes. J Virol. 1981 May;38(2):680–687. doi: 10.1128/jvi.38.2.680-687.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  11. Hawley R. G., Shulman M. J., Murialdo H., Gibson D. M., Hozumi N. Mutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7425–7429. doi: 10.1073/pnas.79.23.7425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heidmann O., Heidmann T. Retrotransposition of a mouse IAP sequence tagged with an indicator gene. Cell. 1991 Jan 11;64(1):159–170. doi: 10.1016/0092-8674(91)90217-m. [DOI] [PubMed] [Google Scholar]
  13. Herrin D. L., Schmidt G. W. Rapid, reversible staining of northern blots prior to hybridization. Biotechniques. 1988 Mar;6(3):196-7, 199-200. [PubMed] [Google Scholar]
  14. Kuff E. L., Feenstra A., Lueders K., Smith L., Hawley R., Hozumi N., Shulman M. Intracisternal A-particle genes as movable elements in the mouse genome. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1992–1996. doi: 10.1073/pnas.80.7.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kuff E. L., Smith L. A., Lueders K. K. Intracisternal A-particle genes in Mus musculus: a conserved family of retrovirus-like elements. Mol Cell Biol. 1981 Mar;1(3):216–227. doi: 10.1128/mcb.1.3.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mietz J. A., Grossman Z., Lueders K. K., Kuff E. L. Nucleotide sequence of a complete mouse intracisternal A-particle genome: relationship to known aspects of particle assembly and function. J Virol. 1987 Oct;61(10):3020–3029. doi: 10.1128/jvi.61.10.3020-3029.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ono M., Cole M. D., White A. T., Huang R. C. Sequence organization of cloned intracisternal A particle genes. Cell. 1980 Sep;21(2):465–473. doi: 10.1016/0092-8674(80)90483-3. [DOI] [PubMed] [Google Scholar]
  18. Paterson B. M., Segal S., Lueders K. K., Kuff E. L. RNA associated with murine intracisternal type A particles codes for the main particle protein. J Virol. 1978 Jul;27(1):118–126. doi: 10.1128/jvi.27.1.118-126.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rechavi G., Givol D., Canaani E. Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like element. Nature. 1982 Dec 16;300(5893):607–611. doi: 10.1038/300607a0. [DOI] [PubMed] [Google Scholar]
  20. Schüpbach T., Wieschaus E. Female sterile mutations on the second chromosome of Drosophila melanogaster. II. Mutations blocking oogenesis or altering egg morphology. Genetics. 1991 Dec;129(4):1119–1136. doi: 10.1093/genetics/129.4.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Silver J. Confidence limits for estimates of gene linkage based on analysis of recombinant inbred strains. J Hered. 1985 Nov-Dec;76(6):436–440. doi: 10.1093/oxfordjournals.jhered.a110140. [DOI] [PubMed] [Google Scholar]
  22. Upton C., Macen J. L., Wishart D. S., McFadden G. Myxoma virus and malignant rabbit fibroma virus encode a serpin-like protein important for virus virulence. Virology. 1990 Dec;179(2):618–631. doi: 10.1016/0042-6822(90)90129-f. [DOI] [PubMed] [Google Scholar]
  23. Wujcik K. M., Morgan R. A., Huang R. C. Transcription of intracisternal A-particle genes in mouse myeloma and Ltk- cells. J Virol. 1984 Oct;52(1):29–36. doi: 10.1128/jvi.52.1.29-36.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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