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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Cytotherapy. 2013 Aug 29;15(12):1527–1540. doi: 10.1016/j.jcyt.2013.04.010

Figure 6. Example of Nile Red staining and adipocyte quantification.

Figure 6

(A) Image of differentiated adipocytes following 21 days induction. Nile Red positive staining (green) visualized as lipid vesicles indicating a differentiated adipocyte; nuclei staining with Hoechst (blue). Image taken during automated imaging at 10X magnification. (B) Following analysis by the macro, Nile Red positive cells were indicated with a green transparent overlay plus associated nuclei (orange). Nuclei not associated with Nile Red staining are shown as blue areas. As an example, the software detected 9 adipocytes in this image. Further analysis would include normalization of this value to total number of nuclei in this image to determine a percent of differentiated cells.