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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Cytotherapy. 2013 Aug 29;15(12):1527–1540. doi: 10.1016/j.jcyt.2013.04.010

Figure 7. Adipogenesis can be quantified by automated microscopy, and demonstrates both donor variability and decreased adipogenic potential with increasing passage.

Figure 7

Figure 7

(A) Nuclei plus Nile Red objects were normalized by the total nuclei count per image to report a percentage of differentiated cells (% Nile Red positive) as a function of passage and cell line. Purple bar = P3; orange bar = P5; green bar = P7. Error bars represent standard deviation; n=3. Statistical comparisons were made within cell lines, relative to P3. (B) Statistical analysis of percent adipogenesis data for comparison between cell lines. Purple shading = P3, orange shading = P5, green shading = P7. For (A) and (B), ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05, ns = not significant, n/a = not applicable.