Gut Catalase-Positive Bacteria Cross-Protect Adjacent Bifidobacteria from Oxidative Stress

Abstract

Bifidobacteria isolated from infant gut and breast milk exhibited different abilities to grow under microaerobic conditions, alone or in the presence of added catalase. In the present study, we demonstrated that some Bifidobacterium strains unable to grow under microaerobic conditions were cross-protected on solid media from oxidative stress by adjacent colonies of gut catalase-positive Staphylococcus epidermidis or Escherichia coli, but not by a catalase-deficient E. coli. The results of this study support the possible contribution of catalase-positive bacteria to the establishment of certain bifidobacteria in non-anaerobic human niches of the infant gastrointestinal tract or mammary gland.

Colonization of the gut is described as a complex process influenced by microbial-host interactions as well as by external and internal factors. Climax intestinal microbiota in newborns is attained in successive stages. The intestinal and vaginal microbiota and breast milk of the mother are the main sources of bacteria for the neonate during the first days of life (6, 14, 15). The first colonizers of the gastrointestinal tract are bacteria that are tolerant to oxygen such as Escherichia coli and other Enterobacteriaceae, enterococci, and staphylococci (4, 5, 10, 21), which are also present at high levels in colostrum and breast milk, whereas bifidobacteria are detected at very low levels (14). In contrast, from day six to one month old, the microbiota becomes stable and dominated by bifidobacteria (4). Bifidobacterium species represent one of the main bacterial groups in the infant gut, particularly in breastfed infants (4, 7, 18), thereby linking the presence of high levels of bifidobacteria with the timely and appropriate development and maturation of the immune system (8, 20). However, the rapid establishment of Bifidobacterium in this environment is a challenge because anoxic conditions are not ensured in the infant gut during the first days following delivery. The exposure of bifidobacteria to oxygen causes oxidative stress due to the accumulation of reactive oxygen species, mainly hydrogen peroxide (H₂O₂), which may lead to cell death (11, 16, 19). Catalase represents the main line of defense against H₂O₂ in many kinds of bacteria. The production of catalase has been reported in Bifidobacterium indicum and Bifidobacterium asteroides asteroids, but is absent in most bifidobacteria (2). According to He et al.(9), the acquired tolerance to oxidative stress of Bifidobacterium longum 105-A via the heterologous expression of a catalase gene revealed that the accumulation of H₂O₂ was a primary factor for the inhibition of growth by bifidobacteria. Previous studies reported that the addition of catalase improved the aerobic growth of bifidobacteria (3, 11). Catalase-positive E. coli has been shown to cross-protect adjacent catalase-deficient (ΔkatE) mutant E. coli K-12 (12) colonies against a H₂O₂ challenge (13).

The aim of the present study was to investigate the tolerance of bifidobacteria strains isolated from infant gut and breast milk to oxygen and determine if catalase-positive bacteria present in human niches exhibited the ability to cross-protect adjacent bifidobacteria from oxidative stress, thereby allowing their survival and multiplication under environmental conditions with exposure to oxygen.

Eighty-five strains of bifidobacteria previously isolated from the infant gut and human breast milk (17) were investigated in the present study (Fig. 1). Strains were grown in Reinforced Clostridial agar (RCA; Difco Laboratories, Sparks, MD, USA) for 48 h at 37°C under anaerobic conditions (AnaeroGen; Oxoid, Basingstoke, Hampshire, UK). Catalase-positive S. epidermidis INIA P190, E. coli INIA P114, and E. coli K-12, which were obtained from the National Institute for Agricultural and Food Research and Technology (INIA) Culture Collection, were grown in Brain Heart Infusion broth (BHI; Merk, Darmstadt, Germany) for 18 h at 37°C. A catalase-deficient mutant (ΔkatE) strain of E. coli K-12 (JW1721-1) (1), which showed no assayable catalase activity, was provided by the E. coli Genetic Stock Center (Yale University, CT, USA). Stock cultures were kept frozen at −80°C in the appropriate broth containing 10% (v/v) glycerol. All strains were subcultured twice before being used in experiments.

Fig. 1.

Distribution and number (in brackets) of bifidobacteria species, according to their ability to grow under microaerobic conditions in the presence/absence of catalase. Class 1: No growth; Class 2: Growth; Class 3: Growth only in the presence of catalase (520 AU mL⁻¹).

Suspensions (at approximately 7 log CFU mL⁻¹) of bifidobacteria were prepared in duplicate by collecting cells from RCA plates and resuspending them in 2 mL of BHI broth to test their tolerance to oxidative stress. Bacterial suspensions were streaked onto BHI agar plates with or without catalase (Catalase type C-10; Sigma-Aldrich, St. Louis, MO, USA) at 0.2 g L⁻¹, equivalent to 520 AU mL⁻¹ agar, and cultured for 48 h at 37°C under microaerobic conditions (GENbox microaer; bioMérieux, Marcy L’Etoile, France) as well as anaerobic and aerobic conditions. Based on their behavior under microaerobic conditions, bifidobacteria were classified into three classes: unable to grow (class 1), able to grow (class 2), and only able to grow in the presence of catalase (class 3).

Representative bifidobacteria strains from the three classes previously described were investigated in mixed culture assays with the breast fed infant gut catalase-positive isolates S. epidermidis INIA P190 and E. coli INIA P114, and with E. coli K-12 and its catalase-deficient (ΔkatE) mutant E. coli JW1721-1. Mixed cultures in pairs combining bifidobacteria (at approximately 7 log CFU mL⁻¹) with each of the catalase-positive strains (at approximately 3 log CFU mL⁻¹) were spread onto BHI agar plates and cultured under micro-aerobic conditions for 48 h at 37°C. The identity of presumptive bifidobacteria colonies was confirmed by observing the characteristic bifid morphology with phase-contrast microscopy and by colony PCR with specific primers and conditions previously described (17).

All the bifidobacteria tested grew well under anaerobic conditions and were unable to grow under aerobic conditions. According to their behavior under microaerobic conditions, bifidobacteria were classified into three classes (Fig. 1). A total of 31% of the strains were not able to grow under microaerobic conditions even when catalase was present (class 1). This group included Bifidobacterium adolescentis (100%), Bifidobacterium catenulatum (80%), and Bifidobacterium pseudocatenulatum (80%). Only 10% of all the strains tested tolerated microaerobic conditions and formed proper colonies (class 2). This group encompassed some strains belonging to Bifidobacterium bifidum (10%), Bifidobacterium breve (20%), and B. longum subsp. infantis (50%). When catalase was added, 59% of the strains tested were able to grow under microaerobic conditions (class 3). This group included strains of all the species tested, except for B. adolescentis. Kawasaki et al.(11) previously reported that the growth of two strains of bifidobacteria, which was inhibited due to the accumulation of H₂O₂, was restored by the addition of catalase to the medium. The bifidobacteria species described in the literature as being common in the first days of life in the infant gut (18) were those exhibiting higher tolerance to oxygen in the present study (B. bifidum, B. breve, and B. longum subsp. infantis), and mainly belonged to classes 2 and 3. These results suggested that some bifidobacteria remained in the microaerobic parts of the intestine and mammary ducts as long as H₂O₂ produced by that species was neutralized. Therefore, we investigated the potential cross-protection of catalase-positive bacteria, typically present in the neonate gut or in human breast milk (10), against H₂O₂ produced by bifidobacteria in the presence of low oxygen levels.

Three representative strains from each of the classes described above, B. longum subsp. longum INIA P721, B. adolescentis INIA P879, and B. pseudocatenulatum INIA P888 from class 1; B. bifidum INIA P671, B. breve INIA P714, and B. longum subsp. infantis INIA P718 from class 2; and B. breve INIA P244, B. bifidum INIA P745, and B. longum subsp. longum INIA P748 from class 3, were studied in mixed culture assays with the catalase-positive isolates in solid media. The bifidobacteria from class 1 did not grow in the presence of catalase-positive bacteria, while those from class 2 grew together with catalase-positive strains over all the agar plate. The strains belonging to class 3 showed small colonies adjacent to the catalase-positive colonies. Typical images of strains from the three classes growing on BHI agar in a mixed culture with S. epidermidis INIA P190, E. coli INIA P114, or E. coli K-12 under microaerobic conditions are shown in Fig. 2 and Fig. S1. The cells from these small colonies showed the typical bifid morphology under phase contrast microscopy (Fig. S2) and were identified as Bifidobacterium sp. by PCR (data not shown). In contrast to the parental strain E. coli K-12, no bifidobacteria colonies belonging to class 3 were observed adjacent to the catalase-deficient (ΔkatE) E. coli mutant during the mixed culture assays carried out with the selected strains, indicating that catalase played a key role in this event (Fig. 2f). According to these results, gut catalase-positive bacteria protected neigh-boring catalase-deficient bifidobacteria against oxidative stress, thereby assisting in their establishment in the infant gastrointestinal tract or other non-anaerobic human niches such as the mammary gland. The gut microbiota is a complex ecosystem in which microorganisms live in a balanced relationship with other species that often occur in dense biofilms. Ma and Eaton (13) supported bacterial catalase protecting high-density or colonial, but not individual E. coli against environmental H₂O₂. This group protection may extend to neighboring catalase-negative E. coli.

Fig. 2.

Growth under microaerobic conditions of Staphylococcus epidermidis INIA P190 in a mixed culture with: (a) class 1 Bifidobacterium adolescentis INIA P879; (b) class 2 Bifidobacterium longum INIA P718; or (c) class 3 Bifidobacterium bifidum INIA P745; Escherichia coli K-12 in a mixed culture with: (d) B. longum INIA P718 or (e) B. bifidum INIA P745; and (f) catalase-deficient mutant E. coli JW1721-1 in a mixed culture with B. bifidum INIA P745. Representative colonies are indicated by arrows with the strain name.

In conclusion, this study shows differences in the tolerance of bifidobacterial species to oxidative stress, pointing out the competitive advantage of some strains of B. bifidum, B. breve, B. longum subsp. infantis, and B. longum subsp. longum in the colonization process of the infant gut microbiota, being the cross-protection of gut catalase-positive bacteria a possible mechanism towards the hydrogen peroxide generated by Bifidobacterium species.