FIG 2.

CDI in mice with or without FMT after vancomycin treatment of primary CDI. (A) Experimental design and timeline of mice with CDI relapse with or without FMT. (B) Mean C. difficile colonization levels (expressed as CFU/g of fecal pellets) of mice with relapse without FMT (blue) or with FMT (green). Error bars represent standard deviations, and the horizontal line indicates the limit of detection. (C) Cytotoxin activity (expressed as the log₁₀ reciprocal cytotoxin dilution per gram of fecal content) was determined using a Vero cell cytotoxicity assay in mice treated with vancomycin only (blue) or vancomycin followed by FMT (green). Points represent samples collected from either mice that received only vancomycin (blue) or mice that also received FMT (green). Bars represent the median per group and lower/upper quartiles. Significance was determined by using a Wilcoxon rank sum test (*, P < 0.05; **, P < 0.005; ***, P < 0.0005).