The following information is missing from the Funding section: This project was supported by the Taichung Veterans General Hospital and National Chung Hsing University (TCVGH-NCHU1047602).
Two references are omitted from the first sentence of the “p17 Drives β-Arrestin-Mediated PTEN Translocation from the Cytoplasm to the Plasma Membrane via a Rock-1 Dependent Manner” subsection of the Results.
The sentence should read: It is well-known that the small GTPase, RhoA, was one of the known proteins to increase PTEN lipid phosphatase activity via its downstream effector Rho kinase (ROCK) [51, 52, Lima-Fernandes et al., 2011, Li et al., 2005].
The references are: Lima-Fernandes E, Enslen H, Camand E, Kotelevets L, Boularan C, Achour L, et al. (2011) Distinct functional outputs of PTEN signalling are controlled by dynamic association with β-arrestins. EMBO J. 30(13):2557–68.
Li Z, Dong X, Wang Z, Liu W, Deng N, Ding Y, et al. (2005) Regulation of PTEN by Rho small GTPases. Nat Cell Biol. 7(4):399–404.
Table 1 has been corrected for improved readability. Please see the corrected Table 1 here.
| Gene | Accession number | Sequence (5′-3′)* | Location | Expected size (bp) |
|---|---|---|---|---|
| Primers for cloning | ||||
| p17 (Flag-tagged, full length) | AF330703 | F: CGGAATTCATGCAATGGCTCCGCCATACGA (EcoR I) | 293–314 | 441 |
| R: GCTCTAGATCATAGATCGGCGTCAAATCGC (Xba I) | 733–712 | |||
| p17 (His-tagged, full length) | AF330703 | F: CCCAAGCTTATGCAATGGCTCCGCCATACGA (Hind III) | 293–314 | 441 |
| R: CCGCTCGAGTCATAGATCGGCGTCAAATCGC (Xho I) | 733–712 | |||
| p17 (27–146) | AF330703 | F: CGGAATTCACAATGGCATCATTTACTGCTATAAC (EcoR I) | 371–390 | 363 |
| R: AACTCGAGTCATAGATCGGCGTCAAATCGCC (Xho I) | 733–711 | |||
| p17 (1–118) | AF330703 | F: CGGAATTCACAATGCAATGGCTCCGCCATACG (EcoR I) | 293–313 | 354 |
| R: AAACTCGAGTCAGGATTGAGACCCGCCATCCCAATG (Xho I) | 646–623 | |||
| p17 (119–146) | AF330703 | F: CGGAATTCACAATGATCGCAGCGAAGAGAGGTCGTC (EcoR I) | 647–668 | 87 |
| R: AACTCGAGTCATAGATCGGCGTCAAATCGCC (Xho I) | 733–711 | |||
| Primers for semi-quantitative RT-PCR | ||||
| p17 (Full length) | AF330703 | F: ATGCAATGGCTCCGCCATACGA | 293–314 | 441 |
| R: TCATAGATCGGCGTCAAATCGC | 733–712 | |||
| Tpr | NM_003292 | F: CACTCTGATCTTGGCCAGCTTG | 6637–6658 | 479 |
| R: CCACGGACACCTTGTCTCAATG | 7115–7094 | |||
| p53 | NM_001047151 | F: CCCTTCCCAGAAAACCTACC | 384–403 | 223 |
| R: CTCCGTCATGTGCTGTGACT | 606–587 | |||
| PTEN | NM_001260965 | F: CGAACTGGTGTAATGATAT | 578–594 | 330 |
| R: CATGAACTTGTCTTCCCGT | 907–889 | |||
| Rak | NM_002031 | F: TGGTCAGTTCGGCGAAGTATGG | 1173–1194 | 346 |
| R: GGCAGCCAGATCTCTGTGAATG | 1518–1497 | |||
| GAPDH | NM_002046 | F: CACCACCATGGAGAAGGCTGGGGCTCA | 480–506 | 454 |
| R: GGCAGGTTTCTCCAGACGGCAGGTCAG | 933–907 | |||
F: forward; R: reverse;
* Underlines indicate the restriction sites designed in the indicated primers
Reference
- 1. Huang W-R, Chiu H-C, Liao T-L, Chuang K-P, Shih W-L, Liu H-J (2015) Avian Reovirus Protein p17 Functions as a Nucleoporin Tpr Suppressor Leading to Activation of p53, p21 and PTEN and Inactivation of PI3K/AKT/mTOR and ERK Signaling Pathways. PLoS ONE 10(8): e0133699 doi:10.1371/journal.pone.0133699 [DOI] [PMC free article] [PubMed] [Google Scholar]