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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Neuroscience. 2015 Aug 8;305:268–278. doi: 10.1016/j.neuroscience.2015.08.012

Fig. 1. Comparison of PVDF Membranes Used to Detect RBM3 by Western Blot.

Fig. 1

(A) Methods used to equalize factors for comparison of PVDF membranes. Neuron homogenates (i.e. samples 1and 2) were loaded onto a 15-well 4–15% SDS gradient gel in five replicates. PVDF membranes were precisely cut to span the width of 2 lanes and match gel length. Proteins were transferred to all 5 membranes at the same time (in the same tank/cassette). Membranes were processed using the same volume/time incubation in blocking solution, TBS washes, primary antibody, secondary antibody, and ECL detection reagent. Membranes were put inside a single film holder for equivalent film exposures in a dark room. (B) Western blots show comparison of PVDF membranes to capture and/or detect RBM3.