IDS Crossing of the Blood-Brain Barrier Corrects CNS Defects in MPSII Mice

(The American Journal of Human Genetics 85, 296–301; August 14, 2009)

In this article, Figure 3A unfortunately showed incorrect immunohistochemistry of the CD68 marker in the thalamus of WT (T18) and Ids^y/−+IDS (T18) mice. The revised figure depicting the correct CD68 immunohistochemistry of WT (T18) and Ids^y/−+IDS (T18) mice, as well as control Ids^y/− (T14) mice, appears below. The authors apologize for the oversight.

Figure 3.

Rescue of Brain Defects in AAV2/5CMV-hIDS-Injected Ids^y/− Mice

(A and B) Immunohistochemistry of different brain-specific markers (monoclonal anti-NeuN, diluted 1:100, Chemicon International; polyclonal anti-ubiquitin, diluted 1:50, Abcam; TUNEL, Chemicon International Staining Kit; monoclonal anti-GFAP, diluted 1:200, Sigma-Aldrich; and monoclonal antiCD68, diluted 1:250, AbD Serotec) in the thalamus and white matter (monoclonal anti-a-synuclein, diluted 1:250, Abcam) of brain sections in T18 WT, T14 Ids^y/−, and T18 AAV2/5CMV-hIDS-injected Ids^y/− mice. Magnification: 10× (anti-NeuN sections) or 20× (all others).