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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Jan 15;90(2):630–634. doi: 10.1073/pnas.90.2.630

A histidine protein kinase is involved in polar organelle development in Caulobacter crescentus.

S P Wang 1, P L Sharma 1, P V Schoenlein 1, B Ely 1
PMCID: PMC45717  PMID: 8421698

Abstract

Mutations having pleiotropic effects on polar organelle development (pod) in Caulobacter crescentus have been identified and shown to occur in at least 13 genes scattered throughout the genome. Mutations at each locus affect a unique combination of polar traits, suggesting that complex interactions occur among these genes. The DNA sequence of one of these genes, pleC, indicates that it is homologous to members of the family of histidine protein kinase genes. Membes of this family include the senor components of the bacterial two-component regulatory systems. Furthermore, in vitro experiments demonstrated that the PleC protein was capable of autophosphorylation. These results suggest that the PleC protein (and perhaps the proteins encoded by the other pod genes as well) regulates the expression of genes involved in polar organelle development through the phosphorylation of key regulatory proteins. The use of a phosphorelay system cued to internal changes in the cell would provide a mechanism for coordinating major changes in gene expression with the completion of specific cell cycle events.

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Selected References

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  1. Aricó B., Miller J. F., Roy C., Stibitz S., Monack D., Falkow S., Gross R., Rappuoli R. Sequences required for expression of Bordetella pertussis virulence factors share homology with prokaryotic signal transduction proteins. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6671–6675. doi: 10.1073/pnas.86.17.6671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dingwall A., Gober J. W., Shapiro L. Identification of a Caulobacter basal body structural gene and a cis-acting site required for activation of transcription. J Bacteriol. 1990 Oct;172(10):6066–6076. doi: 10.1128/jb.172.10.6066-6076.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Driks A., Schoenlein P. V., DeRosier D. J., Shapiro L., Ely B. A Caulobacter gene involved in polar morphogenesis. J Bacteriol. 1990 Apr;172(4):2113–2123. doi: 10.1128/jb.172.4.2113-2123.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ely B., Croft R. H., Gerardot C. J. Genetic mapping of genes required for motility in Caulobacter crescentus. Genetics. 1984 Nov;108(3):523–532. doi: 10.1093/genetics/108.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ely B., Ely T. W. Use of pulsed field gel electrophoresis and transposon mutagenesis to estimate the minimal number of genes required for motility in Caulobacter crescentus. Genetics. 1989 Dec;123(4):649–654. doi: 10.1093/genetics/123.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ely B., Gerardot C. J., Fleming D. L., Gomes S. L., Frederikse P., Shapiro L. General nonchemotactic mutants of Caulobacter crescentus. Genetics. 1986 Nov;114(3):717–730. doi: 10.1093/genetics/114.3.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ely B., Gerardot C. J. Use of pulsed-field-gradient gel electrophoresis to construct a physical map of the Caulobacter crescentus genome. Gene. 1988 Sep 7;68(2):323–333. doi: 10.1016/0378-1119(88)90035-2. [DOI] [PubMed] [Google Scholar]
  10. Fukuda A., Asada M., Koyasu S., Yoshida H., Yaginuma K., Okada Y. Regulation of polar morphogenesis in Caulobacter crescentus. J Bacteriol. 1981 Jan;145(1):559–572. doi: 10.1128/jb.145.1.559-572.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gober J. W., Xu H., Dingwall A. K., Shapiro L. Identification of cis and trans-elements involved in the timed control of a Caulobacter flagellar gene. J Mol Biol. 1991 Jan 20;217(2):247–257. doi: 10.1016/0022-2836(91)90539-i. [DOI] [PubMed] [Google Scholar]
  12. Hager D. A., Burgess R. R. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem. 1980 Nov 15;109(1):76–86. doi: 10.1016/0003-2697(80)90013-5. [DOI] [PubMed] [Google Scholar]
  13. Heimbrook M. E., Wang W. L., Campbell G. Staining bacterial flagella easily. J Clin Microbiol. 1989 Nov;27(11):2612–2615. doi: 10.1128/jcm.27.11.2612-2615.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jin S., Roitsch T., Ankenbauer R. G., Gordon M. P., Nester E. W. The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation. J Bacteriol. 1990 Feb;172(2):525–530. doi: 10.1128/jb.172.2.525-530.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Johnson R. C., Ely B. Analysis of nonmotile mutants of the dimorphic bacterium Caulobacter crescentus. J Bacteriol. 1979 Jan;137(1):627–634. doi: 10.1128/jb.137.1.627-634.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson R. C., Ely B. Isolation of spontaneously derived mutants of Caulobacter crescentus. Genetics. 1977 May;86(1):25–32. doi: 10.1093/genetics/86.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Khambaty F. M., Ely B. Molecular genetics of the flgI region and its role in flagellum biosynthesis in Caulobacter crescentus. J Bacteriol. 1992 Jun;174(12):4101–4109. doi: 10.1128/jb.174.12.4101-4109.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Minnich S. A., Newton A. Promoter mapping and cell cycle regulation of flagellin gene transcription in Caulobacter crescentus. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1142–1146. doi: 10.1073/pnas.84.5.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mullin D. A., Newton A. Ntr-like promoters and upstream regulatory sequence ftr are required for transcription of a developmentally regulated Caulobacter crescentus flagellar gene. J Bacteriol. 1989 Jun;171(6):3218–3227. doi: 10.1128/jb.171.6.3218-3227.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mullin D., Minnich S., Chen L. S., Newton A. A set of positively regulated flagellar gene promoters in Caulobacter crescentus with sequence homology to the nif gene promoters of Klebsiella pneumoniae. J Mol Biol. 1987 Jun 20;195(4):939–943. doi: 10.1016/0022-2836(87)90497-9. [DOI] [PubMed] [Google Scholar]
  21. Schoenlein P. V., Ely B. Characterization of strains containing mutations in the contiguous flaF, flbT, or flbA-flaG transcription unit and identification of a novel fla phenotype in Caulobacter crescentus. J Bacteriol. 1989 Mar;171(3):1554–1561. doi: 10.1128/jb.171.3.1554-1561.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schoenlein P. V., Gallman L. M., Ely B. Use of transmissible plasmids as cloning vectors in Caulobacter crescentus. Gene. 1988 Oct 30;70(2):321–329. doi: 10.1016/0378-1119(88)90204-1. [DOI] [PubMed] [Google Scholar]
  23. Schoenlein P. V., Gallman L. S., Ely B. Organization of the flaFG gene cluster and identification of two additional genes involved in flagellum biogenesis in Caulobacter crescentus. J Bacteriol. 1989 Mar;171(3):1544–1553. doi: 10.1128/jb.171.3.1544-1553.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schoenlein P. V., Lui J., Gallman L., Ely B. The Caulobacter crescentus flaFG region regulates synthesis and assembly of flagellin proteins encoded by two genetically unlinked gene clusters. J Bacteriol. 1992 Oct;174(19):6046–6053. doi: 10.1128/jb.174.19.6046-6053.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sommer J. M., Newton A. Pseudoreversion analysis indicates a direct role of cell division genes in polar morphogenesis and differentiation in Caulobacter crescentus. Genetics. 1991 Nov;129(3):623–630. doi: 10.1093/genetics/129.3.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sommer J. M., Newton A. Turning off flagellum rotation requires the pleiotropic gene pleD: pleA, pleC, and pleD define two morphogenic pathways in Caulobacter crescentus. J Bacteriol. 1989 Jan;171(1):392–401. doi: 10.1128/jb.171.1.392-401.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stout V., Gottesman S. RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli. J Bacteriol. 1990 Feb;172(2):659–669. doi: 10.1128/jb.172.2.659-669.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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