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. 1993 Jan 15;90(2):664–668. doi: 10.1073/pnas.90.2.664

Construction of chimeric alleles with altered specificity at the b incompatibility locus of Ustilago maydis.

A R Yee 1, J W Kronstad 1
PMCID: PMC45724  PMID: 8421703

Abstract

Multiallelic incompatibility systems found in many fungi and plants function to limit inbreeding by mediating self versus nonself recognition. The plant pathogenic fungus Ustilago maydis has a locus called b that governs incompatibility. Two multiallelic genes, bE and bW, are present at the b locus. Fusion of haploid strains carrying different alleles at bE and bW establishes an infectious dikaryon capable of pathogenesis on maize (Zea mays). Cells carrying a single type of b locus, whether haploid or dikaryotic, are nonpathogenic. To identify sequences within the bE gene that determine allelic specificity, targeted gene replacement was employed to produce a series of chimeras between the b1E and b2E alleles. Incompatibility tests with strains carrying the chimeric alleles identified a 30- to 48-amino acid region responsible for specificity. Suprisingly, the chimeras with recombination points within this region had a specificity different from both parent alleles. Overall, these results define an important domain in bE involved in self versus nonself recognition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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