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. 2015 May 16;138(7):1992–2004. doi: 10.1093/brain/awv127

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© The Author (2015). Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Absence of spontaneous EPSCs in TASTPM CA1 pyramidal neurons (4-months-old unless otherwise indicated). (A) Top: Example traces (V_hold = −70 mV) showing spontaneous EPSCs from 4 m TASTPM and age-matched wild-type mice. Asterisks indicate confirmed EPSCs. Bottom: Example miniature EPSCs from 4 m TASTPM and wild-type mice. (B) Mean frequency of spontaneous (s) EPSCs and miniature (m) EPSCs. Sample sizes (WT/TASTPM): spontaneous EPSCs: 2 m: 11/7; 4 m: 7/7; miniature EPSCs: 2 m: 8/5; 4 m: 8/8 animals. Two-way ANOVA revealed a significant main effect of genotype (2 m: P < 0.05; 4 m: P < 0.001) and tetrodotoxin (2 m: P < 0.05; 4 m: P < 0.001). (C) Example evoked EPSCs from CA3–CA1 synapses at 4 m. (D) Paired-pulse ratios (EPSC2_amplitude/EPSC1_amplitude) were lower in TASTPM (n = 8) than wild-type (n = 6) mice at CA3–CA1 synapses. Two-way ANOVA: main effect of interval (P < 0.0001); genotype (P < 0.01). Post hoc significance as indicated. Inset: The proportion of stimuli that failed to evoke a successful EPSC was lower in TASTPM than wild-type. (E) Entorhinal cortical-CA1 synapses (temporoammonic pathway) showed no difference in paired-pulse ratios between wild-type (n = 5) and TASTPM (n = 6). (F) Paired-pulse ratios at CA3–CA1 synapses in 2-month-old wild-type (n = 5) and TASTPM (n = 5) animals. Two-way ANOVA revealed significant main effects of genotype (P = 0.016), and interval (P < 0.0001). (G) First unitary EPSCs at Schaffer collateral (CA3-CA1) synapses are larger in slices from TASTPM (n = 6) than wild-type (n = 8); (P < 0.0001). (H) In contrast, at temporoammonic synapses, the amplitudes of the first unitary EPSCs were statistically identical between TASTPM (n = 6) and wild-type mice (n = 5). (I) Paired-pulse ratios of pharmacologically isolated NMDA receptor-mediated EPSCs at CA3–CA1 synapses were lower in TASTPM (n = 7) than wild-type mice (n = 6). Two-way ANOVA revealed significant main effects of genotype (P = 0.01) and interval (P < 0.0001). In all panels significance by Sidak post hoc analysis indicated as *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. WT = wild-type.