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Protein sequence alignment of Bra r 5.0101 and the Bra r 5.0101 mutants (mu1, mu2, muW) with two EF-hand pollen allergens from birch (Bet v 4), from white goosefoot (Che a 3) and from timothy grass (Phl p 7). The two calcium binding sites are marked by boxes. Dots represent amino acids identical with Bra r 5.0101 and gray boxes mark putative surface exposed cross-reactive amino acids mapped to the surface of Che a 3 (Verdino et al., 2008).
