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LC−MS extracted-ion chromatogram (left), MS/MS fragmentation spectrum (center), and chemical structure (right) of the bicyclic (A) and monocyclic (B) peptide products isolated via streptavidin affinity from cells expressing the contruct Z8C_O2beY. Peaks labeled with * correspond to unrelated multicharged ions from adventitious proteins. Data for the other constructs can be found in Figures S7–S13.
