FIGURE 2.

The S. aureus glyS mRNA leader includes a T-box transcription regulator. (A) (Upper panel) glyS leader mRNA in vivo transcription levels under different nutrition conditions during growth. 16S rRNA was used as control. (Lower panel) T-box sequence confirmation of the amplified bands. (B) The region upstream of glyS gene in S. aureus. Boxed regions indicate putative −35 and −10 promoter sites and the transcription start is indicated as +1. The predicted specifier loop (SL) and T-box regions have a dark gray background. In italics and gray is the potential Ρ-independent transcription terminator region; the ribosome binding site (RBS) and glyS start codon (ATG) are underlined. (C) Dinucleotide-primed initiation of transcription. The upper panel indicates the initiation reaction and the lower panel the elongation reaction. ApU, UpA, and CpU correspond to dinucleotides that were used for each reaction.