Skip to main content
. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Immunity. 2015 Sep 8;43(3):591–604. doi: 10.1016/j.immuni.2015.08.012

Figure 5. Analysis of HIV-specific CD8+ T cell responses during hyperacute HIV infection using MHC class I tetramers.

Figure 5

PBMCs were stained with MHC class I tetramers specific for HIV and antibodies against CD38 and HLA-DR. All plots are gated on CD8+ T cells. Upper panels show frequencies of HIV-specific CD8+ T cells for each HIV tetramer tested. Blue dots depict the frequency of tetramer+ cells co-expressing CD38 and HLA-DR overlaid on total CD8+ T cells (red dots). Data for subjects 208, 079 and 093 are shown in panels (a), (b) and (c), respectively. (d) Flow plots show combined tetramer staining and cytokine secretion measured by ICS. Representative data for one of the four donors tested are shown. (e) Proportion of HIV-specific and CMV-specific tetramer+ cells secreting IFN-γ following optimal peptide stimulation of a peak activation sample. Statistical significance was determined using Mann-Whitney test.