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. 2015 Aug 6;4:e06414. doi: 10.7554/eLife.06414

Figure 4. NMDAR and L-Cav channels mediate TBS-induced LTP at PP → CA1tuft synapses.

(A–D) Representative time course of EPSP amplitude before and after TBSx3+Current was delivered (arrows) in 50 µM AP5 (A), 10 µM nimodipine (B), 50 µM AP5 and 10 µM nimodipine (C), or 50 µM Ni2+ (D). Drugs were applied via the bath during the entire experiment. Top, representative traces (single trials) of EPSP before (1) and 25 min after (2) TBSx3+Current was delivered. The scale bar in A applies to all panels. (E) Summary of the LTP experiments in AP5, nimodipine (Nimo), AP5 and nimodipine (AP5+Nimo), and Ni2+, shown along with the data in control and 20 nM TTX (Figure 3) for comparison. EPSP amplitude is normalized to the average EPSP amplitude before LTP induction. Solid lines and shaded areas represent mean and S.E.M., respectively. (F) Potentiation ratio in different experimental conditions (Control, n = 9; AP5, n = 11; Nimo, n = 9; AP5+Nimo, n = 11; Ni2+, n = 9; 20 nM TTX, n = 8). ##p < 0.01, #p < 0.05 for the effect of time on EPSP amplitude by one-way repeated measures ANOVA. **p < 0.01, *p < 0.05 (all compared to control) by one-way ANOVA with post-hoc means comparison using Tukey's test.

DOI: http://dx.doi.org/10.7554/eLife.06414.016

Figure 4—source data 1. Source data of Figure 4.
DOI: http://dx.doi.org/10.7554/eLife.06414.017
elife06414s006.xlsx (384.7KB, xlsx)
DOI: 10.7554/eLife.06414.017
Figure 4—source data 2. Source data of Figure 4—figure supplement 1.
DOI: http://dx.doi.org/10.7554/eLife.06414.018
elife06414s007.xlsx (130.6KB, xlsx)
DOI: 10.7554/eLife.06414.018

Figure 4.

Figure 4—figure supplement 1. L-Cav channels are not required for synaptic transmission before or after the induction of PP → CA1tuft LTP.

Figure 4—figure supplement 1.

Summary of effects of 10 µM nimodipine (Nimo) on somatically recorded voltage or current in response to single-shock stimulation of the PP before (A, B) or after (C, D) the induction of PP → CA1tuft LTP. (A, B) Scatter plots (nimodipine vs control) and bar graphs (normalized to control) of the amplitude or integral of voltage before LTP induction. (C, D) Scatter plots (nimodipine vs control) and bar graphs (normalized to control) of the amplitude or integral of current after LTP induction. Each point represents data from one cell. Solid lines represent a linear fit to data points, with shaded areas representing the 95% confidence band of the fit. Dashed lines are the unity line.
DOI: http://dx.doi.org/10.7554/eLife.06414.019