Figure 5. Reducing Na_v channel availability reduces the slow synaptic depolarization in distal apical trunk in response to TBS significantly less than blocking NMDAR channels.

(A) Differential effects of 20 nM TTX and 50 µM AP5 on dendritically recorded voltage in response to presynaptic TBS. A₁, Left, experimental configuration showing dendritic whole-cell recording with presynaptic stimulation of the PP. Right, example traces of dendritically recorded voltage in response to presynaptic TBS from a neuron with a large dSpike in control (the same cell as the bottom case shown in Figure 2A) and the corresponding responses in 20 nM TTX or 50 µM AP5 (TTX was washed out before subsequent application of AP5). A₂. Traces corresponding to the box in A₁. Note that the large dSpike was blocked by 20 nM TTX, but not 50 µM AP5. (B) Summary of effects of TTX (n = 9) and AP5 (n = 4) on burst responses (normalized to control). Measured integrals include contributions from large-amplitude dendritic events, which were observed in some cells. **p < 0.01 by Student's t-test.

DOI: http://dx.doi.org/10.7554/eLife.06414.020