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. 2015 Aug 4;7(8):4369–4384. doi: 10.3390/v7082821

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Virus specific IgG were enhanced by H7N9 VLPs or virions. BALB/c mice were vaccinated via intramuscular injection at weeks 0, 2, and 4 with 40 μg of influenza VLPs and WIV or PBS (negative control) only. 96-well plates were coated with inactivated virus, and serum of each group (n = 8) was tested for anti-H7N9 virions specific IgG by ELISA. Data was expressed as mean titer with a standard deviation (SD) bar. H7N9 virions, VLP and PBS: H7N9 virions, H7N9 VLPs, and PBS vaccinated mice serum.