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. 2015 Aug 4;7(8):4369–4384. doi: 10.3390/v7082821

Figure 3.

Figure 3

The antibody isotype of the anti-H7N9 virus antisera. Mice serum was collected from H7N9 virions, H7N9 VLP, and PBS immunized groups (n = 8) at week 6 post vaccination. Inactivated virus was coated in 96-well plates, and the heavy chain (including IgG1, IgG2a, IgG2b, IgG3, and IgM) and light chain (including kappa and lambda) isotypes in antisera were tested by ELISA at a dilution of 1:1000 and reported as specific O.D. H7N9 virions, VLP and PBS: H7N9 virions, H7N9 VLPs, and PBS vaccinated mice serum. *, **, and *** indicate statistical significance (p < 0.05, p < 0.01, and p < 0.001, respectively).