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. 1993 Feb 1;90(3):843–847. doi: 10.1073/pnas.90.3.843

Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.

D Wang 1, D K Hawley 1
PMCID: PMC45766  PMID: 8381534

Abstract

Human RNA polymerase II is shown to be associated with a 3'-->5' exonuclease activity that removes nucleoside 5'-monophosphates from the 3' end of the transcripts in isolated ternary complexes. This activity is stimulated by SII, a protein that acts as a transcription elongation factor in vitro. In addition, we show that another transcription factor, TFIIF, stimulates a competing pyrophosphorolysis reaction. These findings raise interesting questions about the roles of these activities in vivo, including the possibility that this RNA polymerase may proofread the nascent transcript.

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Selected References

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