The Colicin E2 DNase – Im2 interface highlighting interface water molecules and hydrogen bond networks made by water molecules buried at the interface.
(a) Interface water molecules in the DNase-Im2 complex. The protein subunits are illustrated using colored light blue and orange, respectively. The 9 buried interface water molecules are shown as blue spheres, the remaining 14 interface waters as red spheres. Atomic coordinates were taken from reference 50 (PDB-RCSB code 3U43). The illustration was produced using the PyMol software (www.PyMol.org).
(b) The hydrogen bond network formed by the water molecules buried in the interface of the DNase-Im2 complex. Shown are water-water and water protein contacts, formed between the water oxygens and polar heavy atoms of the proteins at a distance of 3.5Å or less.