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. 2015 Sep 11;5(3):489–501. doi: 10.3390/metabo5030489

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Biochemical and chemical similarity network highlighting important differences in metabolites abundance between colonic-cecal content and feces. Vertex size encodes the fold change in metabolite abundance between groups (when the metabolite abundance in colonic-cecal content was higher than that in feces, we defined it as “increase”; in contrast, if the metabolite abundance in colonic-cecal content was lower than that in feces, we defined it as “decrease”). Vertex shape (triangle, increase; “V”, decrease; ellipse, no change) and color (blue, decrease; red, increase; gray, insignificant change or p > 0.05 after-adjustment for FDR) are used to encode the significance and relative direction of changes in metabolites between colonic-cecal content and feces comparison.