Abstract
Objective:
To determine the frequency of ABO blood group and Rhesus (Rh) D antigen in the females of “District” Peshawar, Khyber Pakhtunkhwa Province, Pakistan.
Methods:
This cross-sectional study was conducted on 429 women having pregnancy induced hypertension, admitted in the three teaching hospitals of Peshawar, over a period of one year. Blood sample was collected from each subject after taking informed consent. The antigen antibody agglutination slide test for “blood grouping (ABO)” and RhD factors was done by using IgM and IgG monoclonal reagents. The antisera used were from Biolaboratory, USA. Data was analyzed for percentage calculation.
Results:
The blood group distribution was 134 (31.2%), 43 (10.1%), 116 (27%), 136 (31.7%) for blood groups A, AB, O and B, respectively. Subjects having blood group B was slightly more dominant, followed by A and O, while blood group AB was rare in these females. Blood group A Rh negative is more in female 12 (37.5%) followed by group O 10 (31.3%), group B 09 (28.1%) and group AB 01 (3.1%).
Conclusion:
Frequency of “Rh-positive blood group” is B, A, O and AB, whereas the frequency of the most common Rh-negative blood group are A, O, B and AB respectively. The determination of the frequency of blood groups in the region would not only help in blood transfusion services, but also reduce the risk of erythroblastosis foetalis in the neonates.
KEY WORDS: ABO blood group, Rh factor, Female population
INTRODUCTION
Blood group is genetically predisposed. Until now 400 blood groups are reported but the most important in them are ABO and Rh.1 In transfusion, the most important blood groups are also ABO and Rh blood group system.2
ABO is the human blood group and it depends on presence of A or B genes.3 “ABO blood-group antigens are oligosaccharides attached to cell-surface glycoconjugates expressed by epithelia, endothelia and erythrocytes (RBCs) in primates”.4
The distribution of ABO and Rh blood groups vary from one race to another race; across the world in the population and within human subpopulations. Differences are present even in Pakistan due to racial differences.5 Blood group prevalence plays a role in evolution, genetics research, blood transfusion and organ transplantation. Modern medicine is also working on relationship of blood group with environment.6
The aim of the present study was to find out the frequency of different blood groups in the female population of District Peshawar, Khyber Pakhtunkhwa province Pakistan. Another purpose was to generate data for multipurpose future utilities.
METHODS
A total of 429 females, were screened for blood grouping during a study on pregnancy induced hypertensive women after taking informed consent. Blood samples were taken by aseptic techniques and blood transfer to ethylene diamine tetra acetate (EDTA) containing tubes. The antigen antibody agglutination test was done for determination of blood grouping (ABO) and RhD factor using slide method. Biolaboratory, USA antisera were used in this study. The ABO monoclonal reagents are of hybridized immunoglobulins secreting mouse cell-line. IgM + IgG monoclonal reagents were used for determining RhD factor.
RESULTS
Out of total 429 subjects, RhD positive blood groups were found in 397 (92.5%) and 32 (7.5%) were found to be RhD negative. ABO blood grouping in the subjects is shown in Table-I. Blood group distribution in the total sample was 134 (31.2%), 136 (31.7%), 116 (27%) and 43 (10.1%) for blood groups A, B, O and AB, respectively. The dominant blood group found in our study is blood group B followed by A and O, while blood group AB was rare in these females. Blood group A is common in RhD negative females 12(37.5%) and then group O 10(31.3%), group B 09(28.1%) and group AB 01(3.1%).
Table-I.
ABO blood grouping pattern and RhD factor in females of district Peshawar.
| Blood group | Total subjects N (%) | Rh + N (%) | Rh – N (%) |
|---|---|---|---|
| A | 134(31.2) | 122(30.7) | 12(37.5) |
| B | 136(31.7) | 127(32.0) | 09(28.1) |
| O | 116(27.0) | 106(26.7) | 10(31.3) |
| AB | 43(10.1) | 42(10.6) | 01(3.1) |
| Total | 429(100) | 397(100) | 32(100) |
DISCUSSION
The frequency of ABO and RhD blood group vary from one population to another all over the world. Blood grouping is used in blood transfusion, because it is preferable for patients to receive blood of the same ABO and RhD group. It is also important in determining migration of races and in hereditary diseases.5 Some diseases are more common to develop in certain blood groups; hence relationship of different blood groups with diseases is important.7
Studies in different areas of Pakistan show the incidence and division of ABO and RhD blood groups in different regions.8,9 In our study, group B was more prevalent 136(31.7%), followed by group A 134(31.2%), group O 116(27%) and group AB 43(10.1%). While 92.5% blood groups were RhD positive and 7.5% were RhD negative.
In Pakistan, studies show variations in blood groups in different provinces. A study conducted in Sindh10 showed that blood group O was most prevalent as 36%. Group B (30%), group A (25%) and blood group AB as 9%. A study from “Baluchistan” reported11 blood group AB (7.57%) groups A (21.12%) group B (34.32%), and O as (37.07%).
A study from Rawalpindi and Islamabad12 had shown the percentages of various groups among female subjects, as 32.87% for blood group B, 31.91% for blood group O, 24.02% for blood group A, and 11.20% for blood group AB. Distribution of Rh positive and negative in the considered population was 92.45% and 7.55% respectively.
Blood group B Rh positive female subjects was found to be dominant (28.06%) followed by O (25.5%), A (24.50%) and group AB (9.43%) as reported in a study from Swat,13 Khyber Pakhtunkhwa Province (KPK).
Another study from Bannu KPK14 revealed that the distribution of ABO groups is in the order of 36.23% (B), 31.03% (A), 25.07% (O) and 7.67% (AB). The RhD positive and negative distribution in the studied population was 89.23% and 10.77% respectively.
Punjab and Khyber Pakhtunkhwa Province studies show blood group B as commonest. Our study shows similar results.6-8,15 In Sind and Baluchistan blood group O is found to be dominant which is contrary to our study.10,11,16
Studies conducted in different regions show that blood group O is more prevalent (46%) in USA17, Saudi Arabia18 (52%) and in the population of Iran19 (41.16%). The most prevalent group in Africans is B group while in Australians is O and A is the much commoner.20
In our study 92.5% blood groups were RhD positive and 7.5% were RhD negative. Rh positive male donors are 93% in Saudi Arabia.18 In the USA, 85% of the population are the Rh positive17, while in the British population21 95% are Rh positive.
The result of our study matches with other studies done in different regions of Pakistan. Blood group RhD-Positive blood group is predominant group and its frequency is almost same.
Footnotes
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
Authors’ Contribution
RN: Conceived, designed and did statistical analysis & editing of manuscript.
JH and HA: Did data collection and manuscript writing.
TA and AK: Did review and final approval of manuscript.
TA and RN: Takes the responsibility and is accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
REFERENCES
- 1.Hoffbrand A.V. 2nd edition. London, U.K: Heinemann Professional Publishing Ltd; 1981. Post Graduate Haematology; pp. 270–350. [Google Scholar]
- 2.Molison P.L. 6th edition. Oxford, U.K: Blackwell Scientific Publication; 1979. Blood transfusion in clinical medicine; pp. 239–666. [Google Scholar]
- 3.Daniels G. 2nd edition. Oxford, UK: Blackwell Scientific; 2002. Human blood groups. [Google Scholar]
- 4.Varki A, Cummings R, Esko JD, Freeze H, Stanley P, et al. New York: Cold Spring Harbor Laboratory Press; 2008. Essentials in Glycobiology; p. 784. [PubMed] [Google Scholar]
- 5.Alam M. ABO and Rhesus blood groups in potential blood donors at Skardu (Northern Areas) Pak J Pathol. 2005;16:94–97. [Google Scholar]
- 6.Anees M, Jawad A, Hashmi I. Distribution of ABO and Rh blood group alleles in Mandi bahauddin district of Punjab, Pakistan. Proc. Pakistan Acad. Sci. 2007;44(4):289–294. [Google Scholar]
- 7.Majeed T, Hayee A. Prevalence of ABO blood group and sub groups in Lahore, Punjab (Pakistan) Biomedica. 2002;18:11–15. [Google Scholar]
- 8.Zafar NJ, Hasan K, Bukhari K. Prevalence of ABO and Rh blood group amongst voluntary blood donors. J Rawal Med Coll. 1997;1:78–80. [Google Scholar]
- 9.Mian A, Farooq A. Distribution of ABO and Rh blood group alleles in different population of Southern Punjab. Pakistan Anthropl Anz Man. 1999;57:33–39. [PubMed] [Google Scholar]
- 10.Khaskheli DK, Qureshi AH, Akhund AA. Distribution of ABO and Rh groups in the residents of Sindh. Pak J Health. 1994;31:45–50. [Google Scholar]
- 11.Hussain A, Shiekh SA, Haider M, Rasheed T, Malik MR. Frequency of ABO and Rh blood groups in population of Balouchistan (Pakistan) Pak Armed Forces Med J. 2001;51:22–26. [Google Scholar]
- 12.Khan MS, Farooq N, Qamar N, Tahir F, Subhan F, Kazi BM, et al. Trend of blood groups and Rh Factor in twin cities of Rawalpindi-Islamabad. J Pak Med Assoc. 2006;56(7):299–302. [PubMed] [Google Scholar]
- 13.Khattak ID, Khan TM, Khan P, Shah SMA, Khattak ST, Ali A. Frequency of ABO and rhesus blood groups in district Swat, Pakistan. J Ayub Med Coll Abbottabad. 2008;20(4):127–129. [PubMed] [Google Scholar]
- 14.Khan MS, Subhan F, Tahir F, Kazi BM, Dil AS, Sultan S. Prevalence of blood groups and Rh factor in Bannu region NWFP (Pakistan) Pak J Med Res. 2004;43(1):8–10. [Google Scholar]
- 15.Rahman M, Lodhi Y. Frequency of ABO and Rhesus blood groups in blood donors in Punjab. Pak J Med Sci. 2004;20:315–318. [Google Scholar]
- 16.Bhatti R, Shiekh DM. Variations of ABO Blood groups. Gene Frequencies in Population of Sindh, (Pakistan) Ann King Edward Med Coll. 1999;5:328–331. [Google Scholar]
- 17.Frances TF. In Common Laboratory and Diagnostic Tests. 3rd Edition. Philadelphia: Lippincott; 2002. Blood groups (ABO groups) pp. 19–5. [Google Scholar]
- 18.Bashwari LA, Al Mulhim AA, Ahmad MS, Ahmed MA. Frequency of ABO blood groups in Eastern region of Saudi Arabia. Saudi Med J. 2001;22:1008–1012. [PubMed] [Google Scholar]
- 19.Marzban M, Kamali MS, Hosseinbasi T. Blood groups of the people of Ahwaz, Iran. Anthropol Anz. 1988;46(1):83–89. [PubMed] [Google Scholar]
- 20.Mollison PL, Engelfriet CP. Blood Transfusion in Clinical Medicine. 9th Edition. Oxford: Blackwell Scientific Publication; 1993. ABO, Lewis, Ii and P Groups; pp. 150–151. [Google Scholar]
- 21.Mollison PL, Engelfriet CP, Conteras M. In Blood Transfusion in Clinical Medicine. 9th Edition. Oxford: Black well Scientific Publication; 1993. The Rh blood Group system; pp. 2008–2009. [Google Scholar]