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. 1993 Feb 15;90(4):1523–1525. doi: 10.1073/pnas.90.4.1523

Crystals of hemagglutinin-neuraminidase of parainfluenza virus contain triple-stranded helices.

K G Murti 1, T Takimoto 1, W G Laver 1, A Portner 1
PMCID: PMC45906  PMID: 8381970

Abstract

When purified dimers of hemagglutinin-neuraminidase molecules released by protease digestion from three strains of human parainfluenza virus 1 were used in crystallization trials, long thin needle crystals formed. Electron microscopic analysis of these needle crystals revealed that they are composed of stacks of triple-stranded helices with each strand of the helix made up of subunits of hemagglutinin-neuraminidase. To our knowledge, this is the first direct demonstration of the assembly of protein subunits into large triple-stranded helices. An understanding of the organization of these triple helices may shed light on the structural properties of the hemagglutinin-neuraminidase molecules that cause them to form these helices.

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Selected References

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  1. Air G. M., Laver W. G., Luo M., Stray S. J., Legrone G., Webster R. G. Antigenic, sequence, and crystal variation in influenza B neuraminidase. Virology. 1990 Aug;177(2):578–587. doi: 10.1016/0042-6822(90)90523-t. [DOI] [PubMed] [Google Scholar]
  2. CHANOCK R. M., PARROTT R. H., COOK K., ANDREWS B. E., BELL J. A., REICHELDERFER T., KAPIKIAN A. Z., MASTROTA F. M., HUEBNER R. J. Newly recognized myxoviruses from children with respiratory disease. N Engl J Med. 1958 Jan 30;258(5):207–213. doi: 10.1056/NEJM195801302580502. [DOI] [PubMed] [Google Scholar]
  3. Delage G., Brochu P., Pelletier M., Jasmin G., Lapointe N. Giant-cell pneumonia caused by parainfluenza virus. J Pediatr. 1979 Mar;94(3):426–429. doi: 10.1016/s0022-3476(79)80591-0. [DOI] [PubMed] [Google Scholar]
  4. Frank J. A., Jr, Warren R. W., Tucker J. A., Zeller J., Wilfert C. M. Disseminated parainfluenza infection in a child with severe combined immunodeficiency. Am J Dis Child. 1983 Dec;137(12):1172–1174. doi: 10.1001/archpedi.1983.02140380032010. [DOI] [PubMed] [Google Scholar]
  5. Hay E. D. Extracellular matrix. J Cell Biol. 1981 Dec;91(3 Pt 2):205s–223s. doi: 10.1083/jcb.91.3.205s. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jarvis W. R., Middleton P. J., Gelfand E. W. Parainfluenza pneumonia in severe combined immunodeficiency disease. J Pediatr. 1979 Mar;94(3):423–425. doi: 10.1016/s0022-3476(79)80590-9. [DOI] [PubMed] [Google Scholar]
  7. Laver W. G., Thompson S. D., Murti K. G., Portner A. Crystallization of Sendai virus HN protein complexed with monoclonal antibody Fab fragments. Virology. 1989 Jul;171(1):291–293. doi: 10.1016/0042-6822(89)90541-2. [DOI] [PubMed] [Google Scholar]
  8. Ljungman P., Gleaves C. A., Meyers J. D. Respiratory virus infection in immunocompromised patients. Bone Marrow Transplant. 1989 Jan;4(1):35–40. [PubMed] [Google Scholar]
  9. Long C. G., Li M. H., Baum J., Brodsky B. Nuclear magnetic resonance and circular dichroism studies of a triple-helical peptide with a glycine substitution. J Mol Biol. 1992 May 5;225(1):1–4. doi: 10.1016/0022-2836(92)91020-p. [DOI] [PubMed] [Google Scholar]
  10. Markwell M. A., Fox C. F. Protein-protein interactions within paramyxoviruses identified by native disulfide bonding or reversible chemical cross-linking. J Virol. 1980 Jan;33(1):152–166. doi: 10.1128/jvi.33.1.152-166.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Takimoto T., Laver W. G., Murti K. G., Portner A. Crystallization of biologically active hemagglutinin-neuraminidase glycoprotein dimers proteolytically cleaved from human parainfluenza virus type 1. J Virol. 1992 Dec;66(12):7597–7600. doi: 10.1128/jvi.66.12.7597-7600.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thompson S. D., Laver W. G., Murti K. G., Portner A. Isolation of a biologically active soluble form of the hemagglutinin-neuraminidase protein of Sendai virus. J Virol. 1988 Dec;62(12):4653–4660. doi: 10.1128/jvi.62.12.4653-4660.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weintrub P. S., Sullender W. M., Lombard C., Link M. P., Arvin A. Giant cell pneumonia caused by parainfluenza type 3 in a patient with acute myelomonocytic leukemia. Arch Pathol Lab Med. 1987 Jun;111(6):569–570. [PubMed] [Google Scholar]
  14. Wendt C. H., Weisdorf D. J., Jordan M. C., Balfour H. H., Jr, Hertz M. I. Parainfluenza virus respiratory infection after bone marrow transplantation. N Engl J Med. 1992 Apr 2;326(14):921–926. doi: 10.1056/NEJM199204023261404. [DOI] [PubMed] [Google Scholar]

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