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. 2015 Oct 1;10(10):e0139268. doi: 10.1371/journal.pone.0139268

Correction: Comparative Analysis of Label-Free and 8-Plex iTRAQ Approach for Quantitative Tissue Proteomic Analysis

The PLOS ONE Staff
PMCID: PMC4591008  PMID: 26426961

Tables 1 and 5 are incorrectly aligned due to errors introduced in the production process. The publisher apologizes for the errors. Please see the corrected Tables 1 and 5 here.

Table 1. Overview of the number of peptides and the corresponding proteins as being identified in the individual MS-runs.

Method Sample ID # peptide groups # protein groups
LFQ 3_pTa 5073 1096
11_pTa 5269 1099
16_pTa 5725 1185
19_pTa 3931 937
Mean (SD)_pTa 5000 ± 763 1079 ± 103
9_pT2+ 5360 1130
12_pT2+ 5112 1136
15_pT2+ 5516 1169
17_pT2+ 5485 1155
Mean (SD)_pT2+ 5368 ± 184 1148 ± 18
iTRAQ 10 μg 1859 664
fractionation 6099 2064

Table 5. Comparison of the quantification results at the peptide and protein level for identifications with conflicting expression trends between fractionated iTRAQ and LFQ.

Protein/ Peptide Peptide Log2 Ratio (pT2+ vs. pTa) Protein Log2 Ratio (pT2+ vs. pTa)
Protein Name Peptide sequence used for quantification iTRAQ + fractionation LFQ iTRAQ + fractionation LFQ
Heterochromatin protein 1-binding protein 3 eASYSLIR -0.24 0.03 -0,30 0.72
mDAILTEAIk -0,37 0.32
tIPSWATLSASQLAR -0.30 0.41
SSAVDPEPQVK - 1.31
LEDVLPLAFTR - 0.24
GASGSFVVVQK - 5.12
Actin-related protein 2/3 complex subunit 3 aYLQQLR 0.01 0.61 -0.25 0.37
lIGNMALLPIR -0.43 -0.06
lIGNmALLPIR -0.34 -
Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit 2 sIVEEIEDLVAR 0,21 2.46 -0.06 1.23
eDQVIQLMNAIFSk -0.36 -
fELDTSER 0.15 -
nFESLSEAFSVASAAAVLSHNR -0.12 -
qEIQHLFR 0.09 -
yHVPVVVVPEGSASDTHEQAILR -0.38 0.62
LQVTNVLSQPLTQATVK - 0.33
ISTEVGITNVDLSTVDKDQSIAPK - 1.78
NPILWNVADVVIK - 3.65
YIANTVELR 0.06 3.15
KH domain-containing, RNA-binding, signal transduction-associated protein 1 KDDEENYLDLFSHK - 0.39 -0.25 0.63
ILGPQGNTIK - 0.55
DSLDPSFTHAMQLLTAEIEK - 2.42
SGSMDPSGAHPSVR 0.06 0.68
qPPLPHR -0.56 -
General vesicular transport factor p115 SSQTSGTNEQSSAIVSAR - 0.27 -0.10 0.52
SQLNSQSVEITK - 0.25
NDGVLLLQALTR -0,23 2.44
eQDLQLEELR -0.47 -
qSEDLGSQFTEIFIk 0.31 -
vASSTLLDDRR -0.00007 -

Reference


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