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. 1993 Feb 15;90(4):1576–1579. doi: 10.1073/pnas.90.4.1576

In vitro selection of optimal DNA substrates for T4 RNA ligase.

K Harada 1, L E Orgel 1
PMCID: PMC45917  PMID: 8434020

Abstract

We have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 RNA ligase. We find that the ensemble of selected sequences ligated about 10 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly, the majority of the selected sequences approximated a well-defined consensus sequence.

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Selected References

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