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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: J Immunol. 2015 Sep 4;195(8):3992–4000. doi: 10.4049/jimmunol.1501426

Figure 4.

Figure 4

Kappa L chain CDR3 lengths of newborn and adult. Top. Flow chart of end-labeling procedure. Kappa V region PCR products are generated from an RNA pool of two newborn spleens and from adult PBL RNA. The fragments are purified and incubated with Ava II, followed by end-labeling with 32P-dCTP (see Materials and Methods). Bottom. Electrophoresis of denatured fragments. The samples were loaded next to M13mp18 phage (first four lanes labeled GATC) sequenced with −40 primer. The sizes of the shark fragments and their CDR3 were determined by the phage strand size plus labeled nucleotide (101–116 bases plus 1). The other halves of the Ava II-digested fragments were less well separated and not shown.