Table III.
Kappa gene expression in pup sIgM+ cells.
| Shark-LA | Shark-EC | |||
|---|---|---|---|---|
| Total RNA | non- productivea |
non- productiveb |
in-frameb | |
| Igk VkRE18 | 15 | - | - | - |
| VkR4 | 6 | - | - | - |
| VkR20 | 1 | - | - | - |
| VkR18 | 2 | - | - | - |
| Vk5 | 17 (15 unique) | 18 | 0 | 0 |
| Vk2b | 0 | 3 | 14 | 26 (23 unique) |
| Vk16 | 2 | 2 | 0 | 0 |
| Vk53 | 11 (10 unique) | 11 | 5 | 10 |
| Vk43 | 6 | 0 | 1 | 3 |
| Vk45 | 2 | 2 | 1 | 3 (2 unique) |
| Vk6b | 1 | 0 | 0 | 0 |
| Vk30 | 0 | 1 | 0 | 0 |
| Vk50 | 0 | 0 | 2 | 1 |
| Vk52 | 0 | 0 | 5 | 6 |
| Vk36 | 0 | 0 | 5 | 1 |
| unknown | 3 | 1 | 2 | 3 |
| total | 66 | 38 | 34 | 53 |
| splitIgk | 42 (39 unique) | 38 | 34 | 53 (50 unique) |
| GL-joined Igk (% of total) | 24 (36%) | - | - | - |
a
An independent PCR reaction was performed to amplify non-functional rearrangements, which were 39% of the 97 analyzed clones. The in-frame and GL-joined sequences were discarded.
b
One PCR reaction was performed, of which only split gene rearrangements were scored.