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. 1993 Mar 1;90(5):1647–1651. doi: 10.1073/pnas.90.5.1647

Sequencing two DNA templates in five channels by digital compression.

M Nelson 1, Y Zhang 1, D L Steffens 1, R Grabherr 1, J L Van Etten 1
PMCID: PMC45936  PMID: 8446578

Abstract

By applying algebraic coding methods to the Sanger dideoxynucleotide procedure, DNA sequences of two templates can be determined simultaneously in only five reactions and data channels. A 5:2 data compression is accomplished by instantaneous source coding of nucleotide sequence pairs into one set of 5-bit block codes. A general algebraic expression, 2n-1 > or = 4f, describes conditions under which f DNA templates can be sequenced using n channels. Such compression sequencing is accurate and efficient, as demonstrated by manual 35S autoradiographic detection and automated on-line analysis using fluorescent-labeled primers. Symmetric 5:2 compression is especially useful when comparing two closely related sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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