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. 1982 Sep;37(9):703–710. doi: 10.1136/thx.37.9.703

Distribution of pulmonary cholinergic nerves in the rabbit.

A W El-Bermani, E I Bloomquist, J A Montvilo
PMCID: PMC459411  PMID: 7157224

Abstract

Investigations of the nerves of the rabbit lung, by light and electron microscopy, showed a dense acetylcholinesterase-positive innervation of the bronchi through the bronchiolar level. Large nerve bundles were found to decrease in size as they progressed from extrachondral to subchondral connective tissue, forming complex networks of mostly terminal fibres in the muscle layer. In several instances single fibres penetrated the submucosal layer and approached the mucosa. Gangliocytes, which also reacted positively for cholinesterase, were visible in the vicinity of the large peribronchial bundles. Gangliocytes rarely were seen in association with the vasculature. Blood vessels received a much less dense cholinesterase-positive nerve supply than the bronchi. Single, non-terminal fibres were noted at the adventitiomedial junction of the pulmonary artery and vein. In addition, segments of nerve fibres (networks) were observed in the arterial and venous smooth muscle layers. Cholinesterase-positive innervation was even less extensive in the veins than in the arteries.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altenähr E. Untersuchungen über die Feinstruktur der vegetativen Innervation der Rattenlunge. Z Mikrosk Anat Forsch. 1965;72(4):439–518. [PubMed] [Google Scholar]
  2. Bradley D. E., McNary W. F., el-Bermani A. W. The distribution of acetylcholinesterase and catecholamine containg nerves in the rat lung. Anat Rec. 1970 Jun;167(2):205–207. doi: 10.1002/ar.1091670208. [DOI] [PubMed] [Google Scholar]
  3. Cech S. Cholinesterase-containing nerve fibres on blood vessels in lungs of some laboratory mammals. Z Zellforsch Mikrosk Anat. 1973 Jun 20;140(1):91–100. [PubMed] [Google Scholar]
  4. De Troyer A., Borenstein S., Cordier R. Analysis of lung volume restriction in patients with respiratory muscle weakness. Thorax. 1980 Aug;35(8):603–610. doi: 10.1136/thx.35.8.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Delpierre S., Fornaris E., Fornaris M., Grimaud C. Pulmonary mechanics during hypoxia in spontaneously breathing anesthetized rabbits. J Auton Nerv Syst. 1980 Dec;2(4):391–401. doi: 10.1016/0165-1838(80)90036-3. [DOI] [PubMed] [Google Scholar]
  6. Ehinger B., Falck B., Persson H., Sporrong B. Adrenergic and cholinesterase-containing neurons of the heart. Histochemie. 1968;16(3):197–205. doi: 10.1007/BF00307848. [DOI] [PubMed] [Google Scholar]
  7. El-Bermani A. W., Grant M. Acetylcholinesterase-positive nerves of the rhesus monkey bronchial tree. Thorax. 1975 Apr;30(2):162–170. doi: 10.1136/thx.30.2.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eränkö O., Rechardt L., Eränkö L., Cunningham A. Light and electron microscopic histochemical observations on cholinesterase-containing sympathetic nerve fibres in the pineal body of the rat. Histochem J. 1970 Nov;2(6):479–489. doi: 10.1007/BF01003126. [DOI] [PubMed] [Google Scholar]
  9. Fillenz M. Innervation of pulmonary and bronchial blood vessels of the dog. J Anat. 1970 May;106(Pt 3):449–461. [PMC free article] [PubMed] [Google Scholar]
  10. Fisher A. W. The intrinsic innervation of the pulmonary vessels. Acta Anat (Basel) 1965;60(4):481–496. doi: 10.1159/000142658. [DOI] [PubMed] [Google Scholar]
  11. Graham J. D., Lever J. D., Spriggs T. L. An examination of adrenergic axons around pancreatic arterioles of the cat for the presence of acetylcholinesterase by high resolution autoradiographic and histochemical methods. Br J Pharmacol Chemother. 1968 May;33(1):15–20. doi: 10.1111/j.1476-5381.1968.tb00469.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grande N. R., Cohen dos Santos T. New aspects of cholinergic innervation of the canine lung. Arch Anat Histol Embryol. 1972;55(2):399–401. [PubMed] [Google Scholar]
  13. Guz A., Noble M. I., Widdicombe J. G., Trenchard D., Mushin W. W., Makey A. R. The role of vagal and glossopharyngeal afferent nerves in respiratory sensation, control of breathing and arterial pressure regulation in conscious man. Clin Sci. 1966 Feb;30(1):161–170. [PubMed] [Google Scholar]
  14. HONDA Y., NOMURA H., MINOGUCHI M. Effects of vagotomy on the excitability of the respiratory center to blood CO2. Jpn J Physiol. 1957 Jun 15;7(2):137–146. doi: 10.2170/jjphysiol.7.137. [DOI] [PubMed] [Google Scholar]
  15. HONJIN R. Experimental degeneration of the vagus, and its relation to the nerve supply of the lung of the mouse, with special reference to the crossing innervation of the lung by the vagi. J Comp Neurol. 1956 Nov;106(1):1–19. doi: 10.1002/cne.901060102. [DOI] [PubMed] [Google Scholar]
  16. HONJIN R. On the nerve supply of the lung of the mouse, with special reference to the structure of the peripheral vegetative nervous system. J Comp Neurol. 1956 Oct;105(3):587–625. doi: 10.1002/cne.901050308. [DOI] [PubMed] [Google Scholar]
  17. Hetzel M. R., Clark T. J. Comparison of normal and asthmatic circadian rhythms in peak expiratory flow rate. Thorax. 1980 Oct;35(10):732–738. doi: 10.1136/thx.35.10.732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hung K. S. Fine structure of tracheo-bronchial epithelial nerves of the cat. Anat Rec. 1976 May;185(1):85–91. doi: 10.1002/ar.1091850108. [DOI] [PubMed] [Google Scholar]
  19. Hung K. S., Loosli C. G. Electron-microscopic studies of the innervation of the pulmonary veins of the mouse. Acta Anat (Basel) 1977;97(1):97–102. doi: 10.1159/000144720. [DOI] [PubMed] [Google Scholar]
  20. KARNOVSKY M. J., ROOTS L. A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES. J Histochem Cytochem. 1964 Mar;12:219–221. doi: 10.1177/12.3.219. [DOI] [PubMed] [Google Scholar]
  21. Knight D. S., Hyman A. L., Ellison J. P., Kadowitz P. J. Catecholamine mechanisms in the canine and feline lung. Chest. 1977 Feb;71(2 Suppl):262–263. doi: 10.1378/chest.71.2_supplement.262. [DOI] [PubMed] [Google Scholar]
  22. Kokko A., Mautner H. G., Barrnett R. J. Fine structural localization of acetylcholinesterase using acetyl-beta-methylthiocholine and acetylselenocholine as substrates. J Histochem Cytochem. 1969 Oct;17(10):625–640. doi: 10.1177/17.10.625. [DOI] [PubMed] [Google Scholar]
  23. Korner P. I., Shaw J., West M. J., Oliver J. R., Hilder R. G. Integrative reflex control of heart rate in the rabbit during hypoxia and hyperventilation. Circ Res. 1973 Jul;33(1):63–73. doi: 10.1161/01.res.33.1.63. [DOI] [PubMed] [Google Scholar]
  24. Lauweryns J. M., Cokelaere M., Deleersynder M., Liebens M. Intrapulmonary neuro-epithelial bodies in newborn rabbits. Influence of hypoxia, hyperoxia, hypercapnia, nicotine, reserpine, L-DOPA and 5-HTP. Cell Tissue Res. 1977 Sep 5;182(4):425–440. doi: 10.1007/BF00219827. [DOI] [PubMed] [Google Scholar]
  25. Lauweryns J. M., Cokelaere M., Theunynck P. Neuro-epithelial bodies in the respiratory mucosa of various mammals. A light optical, histochemical and ultrastructural investigation. Z Zellforsch Mikrosk Anat. 1972;135(4):569–592. doi: 10.1007/BF00583438. [DOI] [PubMed] [Google Scholar]
  26. Machado A. S., Lemos V. P. Histochemical evidence for a cholinergic sympathetic innervation of the rat pineal body. J Neurovisc Relat. 1971;32(2):104–111. doi: 10.1007/BF02310043. [DOI] [PubMed] [Google Scholar]
  27. Mann S. P. The innervation of mammalian bronchial smooth muscle: the localization of catecholamines and cholinesterases. Histochem J. 1971 Sep;3(5):319–331. doi: 10.1007/BF01005014. [DOI] [PubMed] [Google Scholar]
  28. NADEL J. A., WIDDICOMBE J. G. Effect of changes in blood gas tensions and carotid sinus pressure on tracheal volume and total lung resistance to airflow. J Physiol. 1962 Aug;163:13–33. doi: 10.1113/jphysiol.1962.sp006956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Pillsbury H. R., 3rd, Guazzi M., Freis E. D. Vagal afferent depressor nerves in the rabbit. Am J Physiol. 1969 Sep;217(3):768–770. doi: 10.1152/ajplegacy.1969.217.3.768. [DOI] [PubMed] [Google Scholar]
  30. SPENCER H., LEOF D. THE INNERVATION OF THE HUMAN LUNG. J Anat. 1964 Oct;98:599–609. [PMC free article] [PubMed] [Google Scholar]
  31. Sant'Ambrogio G., Miserocchi G., Mortola J. Transient responses of pulmonary stretch receptors in the dog to inhalation of carbon dioxide. Respir Physiol. 1974 Oct;22(1-2):191–197. doi: 10.1016/0034-5687(74)90057-7. [DOI] [PubMed] [Google Scholar]
  32. Schoener E. P., Frankel H. M. Effect of hyperthermia and Pa CO2 on the slowly adapting pulmonary stretch receptor. Am J Physiol. 1972 Jan;222(1):68–72. doi: 10.1152/ajplegacy.1972.222.1.68. [DOI] [PubMed] [Google Scholar]
  33. Thomson N. C., Kerr J. W. Effect of inhaled H1 and H2 receptor antagonist in normal and asthmatic subjects. Thorax. 1980 Jun;35(6):428–434. doi: 10.1136/thx.35.6.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Woldring S. Interrelation between lung volume, arterial CO2 tension, and respiratory activity. J Appl Physiol. 1965 Jul;20(4):647–652. doi: 10.1152/jappl.1965.20.4.647. [DOI] [PubMed] [Google Scholar]
  35. Yu D. Y., Galant S. P., Gold W. M. Inhibition of antigen-induced bronchoconstriction by atropine in asthmatic patients. J Appl Physiol. 1972 Jun;32(6):823–828. doi: 10.1152/jappl.1972.32.6.823. [DOI] [PubMed] [Google Scholar]
  36. el-Badawi A., Schenk E. A. Dual innervation of the mammalian urinary bladder. A histochemical study of the distribution of cholinergic and adrenergic nerves. Am J Anat. 1966 Nov;119(3):405–427. doi: 10.1002/aja.1001190305. [DOI] [PubMed] [Google Scholar]

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