Figure 5.

amn¹ Mutant flies housed in populations show a fragmented sleep pattern, similar to that of amn¹ individuals. Sleep profiles of amn¹ mutant flies compared with wild-type Canton S flies in individuals (A) and populations (B), respectively. C, Quantification of data. amn¹ mutant flies slept less than wild-type flies in population as well as individuals. D, Activity during waking. Populations of mutant flies were hyperactive during the light period but hypoactive in the dark compared with controls; however, no difference was detected in individual flies. E, Number of sleep episodes. Sleep episodes increased significantly in populations of amn¹ mutant flies compared with controls at night consistent with individuals, but exhibited the opposite phenotype during light period. F, Episode length. Populations of mutant flies did not show significant difference in sleep episode length where individual mutant flies decreased dramatically compared to wild-type. G, Latency. amn¹ mutant flies exhibited similar latency compared to wild type flies at night in both individuals and populations. n = 8 groups for both wild-type and amn¹ populations. n = 31 and n = 32, respectively, for wild-type and amn¹ individuals. ZT, Zeitgeber time.