Figure 4.

Genetic dissection of FLO11-independent ethanol-only invasion by backcrossing of Segregant 3 to BY. (A) Genome-wide relative allele frequency plot of ethanol-only invasion in the backcross of Segregant 3 to BY. The marker used to generate haploid progeny is highlighted with a red vertical bar, while the enriched locus on chromosome II is highlighted with a green vertical bar. The genomic interval underlying the chromosome II locus is also provided. (B) Comparison of Segregant 3, a FLO11-independent ethanol-only FLO8^BY individual, to AMN1^BY replacement strains in the Segregant 3 background supports AMN1 as the causal gene underlying the chromosome II locus.