(
A–
H) Expression of different GFP halves (spGFP11 and spGFP1-10) in putative connected neuronal pairs. aJO-LexA drove expression of spGFP11 in the JO neurons and spGFP1-10 expression was driven by the following spGAL4 lines: (
A) aBN1-spGAL4-1, (
B) aBN2-spGAL4-1, (
C) aDN1-spGAL4-1, (
D) aDN2-spGAL4-2, or (
E) spGAL4 control. Brains were stained with an antibody that recognizes reconstituted GFP (green) (
Gordon and Scott, 2009). The neuropil was stained with an anti-bruchpilot antibody (magenta). GRASP where neurons contacted each other. (
F–
H) Controls where only GFP1-10 was expressed by: (
F) aBN1-spGAL4-1 or (
G) aBN2-spGAL4-1, or (
H) aDN1-spGAL4-1. (
I) White box marks the area shown in
A–
H. Scale bar, 100 μm.