Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Mar 1;90(5):1952–1956. doi: 10.1073/pnas.90.5.1952

Developmental expression of the platelet-derived growth factor alpha-receptor gene in mammalian central nervous system.

H J Yeh 1, I Silos-Santiago 1, Y X Wang 1, R J George 1, W D Snider 1, T F Deuel 1
PMCID: PMC45998  PMID: 8446614

Abstract

We recently reported that the platelet-derived growth factor (PDGF) A-chain gene is highly expressed in neurons of embryonic and adult mouse central nervous system and suggested that its secretion by neurons may support development and maintenance of glia. We have now analyzed the levels and sites of expression of the cognate PDGF alpha-receptor gene in brain and spinal cord of embryonic and adult mice by in situ hybridization. The predominant cell populations in both gray and white matter expressing transcripts of the PDGF alpha-receptor gene are glial cells or their precursors. Transcripts consistently were not detected in neurons. Expression of the PDGF alpha-receptor gene was first observed at embryonic day 15, increased through postnatal day 14, and fell to lower levels in adults. Expression of the alpha-receptor gene corresponds in temporal sequence to the developmental period of glial migration and proliferation and to the expression of PDGF A by neurons. The results indicate that glia but not neurons have the potential to respond to PDGF A and suggest that neurons influence glial cell development through paracrine regulation.

Full text

PDF
1952

Images in this article

1953Fig. 1
on p.1953
1954Fig. 2
on p.1954
1954Fig. 3
on p.1954
1955Fig. 4
on p.1955
1956Fig. 5
on p.1956

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abney E. R., Bartlett P. P., Raff M. C. Astrocytes, ependymal cells, and oligodendrocytes develop on schedule in dissociated cell cultures of embryonic rat brain. Dev Biol. 1981 Apr 30;83(2):301–310. doi: 10.1016/0012-1606(81)90476-0. [DOI] [PubMed] [Google Scholar]
  2. Armstrong R. C., Harvath L., Dubois-Dalcq M. E. Type 1 astrocytes and oligodendrocyte-type 2 astrocyte glial progenitors migrate toward distinct molecules. J Neurosci Res. 1990 Nov;27(3):400–407. doi: 10.1002/jnr.490270319. [DOI] [PubMed] [Google Scholar]
  3. Bejcek B. E., Li D. Y., Deuel T. F. Transformation by v-sis occurs by an internal autoactivation mechanism. Science. 1989 Sep 29;245(4925):1496–1499. doi: 10.1126/science.2551043. [DOI] [PubMed] [Google Scholar]
  4. Claesson-Welsh L., Eriksson A., Westermark B., Heldin C. H. cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor. Proc Natl Acad Sci U S A. 1989 Jul;86(13):4917–4921. doi: 10.1073/pnas.86.13.4917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deuel T. F., Senior R. M., Huang J. S., Griffin G. L. Chemotaxis of monocytes and neutrophils to platelet-derived growth factor. J Clin Invest. 1982 Apr;69(4):1046–1049. doi: 10.1172/JCI110509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dubois-Dalcq M. Characterization of a slowly proliferative cell along the oligodendrocyte differentiation pathway. EMBO J. 1987 Sep;6(9):2587–2595. doi: 10.1002/j.1460-2075.1987.tb02549.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heldin C. H., Johnsson A., Wennergren S., Wernstedt C., Betsholtz C., Westermark B. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature. 1986 Feb 6;319(6053):511–514. doi: 10.1038/319511a0. [DOI] [PubMed] [Google Scholar]
  8. Levi-Montalcini R. The nerve growth factor 35 years later. Science. 1987 Sep 4;237(4819):1154–1162. doi: 10.1126/science.3306916. [DOI] [PubMed] [Google Scholar]
  9. Lillien L. E., Sendtner M., Rohrer H., Hughes S. M., Raff M. C. Type-2 astrocyte development in rat brain cultures is initiated by a CNTF-like protein produced by type-1 astrocytes. Neuron. 1988 Aug;1(6):485–494. doi: 10.1016/0896-6273(88)90179-1. [DOI] [PubMed] [Google Scholar]
  10. Matsui T., Heidaran M., Miki T., Popescu N., La Rochelle W., Kraus M., Pierce J., Aaronson S. Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes. Science. 1989 Feb 10;243(4892):800–804. doi: 10.1126/science.2536956. [DOI] [PubMed] [Google Scholar]
  11. Matsui T., Pierce J. H., Fleming T. P., Greenberger J. S., LaRochelle W. J., Ruggiero M., Aaronson S. A. Independent expression of human alpha or beta platelet-derived growth factor receptor cDNAs in a naive hematopoietic cell leads to functional coupling with mitogenic and chemotactic signaling pathways. Proc Natl Acad Sci U S A. 1989 Nov;86(21):8314–8318. doi: 10.1073/pnas.86.21.8314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Noble M., Murray K., Stroobant P., Waterfield M. D., Riddle P. Platelet-derived growth factor promotes division and motility and inhibits premature differentiation of the oligodendrocyte/type-2 astrocyte progenitor cell. Nature. 1988 Jun 9;333(6173):560–562. doi: 10.1038/333560a0. [DOI] [PubMed] [Google Scholar]
  13. Purves D., Snider W. D., Voyvodic J. T. Trophic regulation of nerve cell morphology and innervation in the autonomic nervous system. Nature. 1988 Nov 10;336(6195):123–128. doi: 10.1038/336123a0. [DOI] [PubMed] [Google Scholar]
  14. Raff M. C., Abney E. R., Miller R. H. Two glial cell lineages diverge prenatally in rat optic nerve. Dev Biol. 1984 Nov;106(1):53–60. doi: 10.1016/0012-1606(84)90060-5. [DOI] [PubMed] [Google Scholar]
  15. Raff M. C. Glial cell diversification in the rat optic nerve. Science. 1989 Mar 17;243(4897):1450–1455. doi: 10.1126/science.2648568. [DOI] [PubMed] [Google Scholar]
  16. Raff M. C., Miller R. H., Noble M. A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature. 1983 Jun 2;303(5916):390–396. doi: 10.1038/303390a0. [DOI] [PubMed] [Google Scholar]
  17. Senior R. M., Griffin G. L., Huang J. S., Walz D. A., Deuel T. F. Chemotactic activity of platelet alpha granule proteins for fibroblasts. J Cell Biol. 1983 Feb;96(2):382–385. doi: 10.1083/jcb.96.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Snider W. D., Johnson E. M., Jr Neurotrophic molecules. Ann Neurol. 1989 Oct;26(4):489–506. doi: 10.1002/ana.410260402. [DOI] [PubMed] [Google Scholar]
  19. Sommer I., Schachner M. Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system. Dev Biol. 1981 Apr 30;83(2):311–327. doi: 10.1016/0012-1606(81)90477-2. [DOI] [PubMed] [Google Scholar]
  20. Yeh H. J., Ruit K. G., Wang Y. X., Parks W. C., Snider W. D., Deuel T. F. PDGF A-chain gene is expressed by mammalian neurons during development and in maturity. Cell. 1991 Jan 11;64(1):209–216. doi: 10.1016/0092-8674(91)90222-k. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES